The Arabidopsis SERK1 protein interacts with the AAA-ATPase AtCDC48, the 14-3-3 protein GF14λ and the PP2C phosphatase KAPP

Rienties, I.M.; Vink, Josefien; Borst, Jan Willem; Russinova, Eugenia; Vries, S.C. de

doi:10.1007/s00425-004-1447-7

Cited by 63 publications

(72 citation statements)

References 44 publications

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“…All of the interacting 14-3-3 proteins localize to both the nucleus and the cytosol. 14-3-3 proteins were also shown to interact with BAK1, its homolog SERK1, and the barley (Hordeum vulgare) homolog Hv BAK1 (Rienties et al, 2005;Karlova et al, 2006;Schoonheim et al, 2007), further supporting important roles of 14-3-3 proteins in BR signaling. 14-3-3 binding has recently emerged as a key regulatory mechanism in plant signaling as well as in yeast and animal systems.…”

Section: Discussionmentioning

confidence: 81%

Nucleocytoplasmic Shuttling of BZR1 Mediated by Phosphorylation Is Essential inArabidopsisBrassinosteroid Signaling

et al. 2007

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Phytohormone brassinosteroids (BRs) play critical roles in plant growth and development. BR acts by modulating the phosphorylation status of two key transcriptional factors, BRI1 EMS SUPPRESSOR1 and BRASSINAZOLE RESISTANT1 (BZR1), through the action of BRASSINOSTEROID INSENSITIVE1/BRI1 ASSOCIATED RECEPTOR KINASE1 receptors and a GSK3 kinase, BRASSINOSTEROID INSENSITIVE2 (BIN2). It is still unknown how the perception of BR at the plasma membrane connects to the expression of BR target genes in the nucleus. We show here that BZR1 functions as a nucleocytoplasmic shuttling protein and GSK3-like kinases induce the nuclear export of BZR1 by modulating BZR1 interaction with the 14-3-3 proteins. BR-activated phosphatase mediates rapid nuclear localization of BZR1. Besides the phosphorylation domain for 14-3-3 binding, another phosphorylation domain in BZR1 is required for the BIN2-induced nuclear export of BZR1. Mutations of putative phosphorylation sites in two distinct domains enhance the nuclear retention of BZR1 and BR responses in transgenic plants. We propose that the spatial redistribution of BZR1 is critical for proper BR signaling in plant growth and development.

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Section: Discussionmentioning

confidence: 81%

Nucleocytoplasmic Shuttling of BZR1 Mediated by Phosphorylation Is Essential inArabidopsisBrassinosteroid Signaling

et al. 2007

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“…In a previous study it was demonstrated using a yeast-two hybrid assay that a leucine rich receptor (LRR) somatic embryogeneis receptor (SERK1) interacts with cell division cycle 48 (CDC48) and with 14-3-3λ (Rienties et al, 2005). CDC48 is expressed throughout the plants in regions of cell division such as the vegetative shoot, root, floral inflorescence and flowers, and in rapidly growing cells (Rienties et al, 2005). 14-3-3 has also been shown to regulate activity of mitochondrial and chloroplast F 0 F 1 ATP synthase via a phosphorylation-dependent binding.…”

Section: Discussionmentioning

confidence: 99%

“…Tensile strength tests suggest its high cell density results in increased cellulose content but the cell wall architecture in 14-3-3λ mutants is altered and, hence emphasizes its role in cell wall development. This gene has a pivotal role in maintaining integrity of plant cells during drought stress particularly since it has been shown to interact with cell division genes such as CDC 48 and SERK1 (Rienties et al, 2005). The collapse of the epidermal, endodermal and cortex cells in the roots of the 14-3-3 mutants also provide evidence of its role in root development.…”

Section: Discussionmentioning

confidence: 99%

Physiological and Mechanical Role of 14-3-3 Lambda in Arabidopsis thaliana during Drought Stress

Peethambaran¹,

Li²,

Dzugan³

et al. 2012

JAS

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Climate changes this century due to global warming compel a greater understanding of molecular mechanisms for plant resistance to drought conditions. Plants undergo changes in their anatomy, morphology, mechanical properties and also in their gene expression in response to environmental stress. Using reverse genetics, anatomy and molecular biology approaches, the role of a 14-3-3 protein isoform in the presence of simulated drought conditions in the model organism Arabidopsis thaliana was investigated. The14-3-3 proteins serve the function of signal transduction in eukaryotes and their role in several abiotic and biotic stress conditions have been previously reported. In this study we used 12 isoforms of 14-3-3 that are expressed and well characterized in A. thaliana to determine which isoform of 14-3-3 contributed to drought stress resistance. A phenotypic assay for drought was conducted by growing all the mutants of the isoforms in media with Abscisic acid (ABA). The 14-3-3 lambda (λ) mutants showed deficiency in lateral root growth. Further investigations revealed significant differences in ion leakage, absolute water content, water potential and tensile strength in mutants deficient in 14-3-3λ versus wild-type (Columbia-0). Leaf anatomy of the 14-3-3λ mutants demonstrated a greater cell density and less intracellular airspace compared to Col-0 in well-hydrated and drought conditions. Roots of the 14-3-3λ mutants exhibited collapsed epidermal, endodermal and cortical cells in both wet and dry conditions. We thus conclude that 14-3-3λ is involved in drought tolerance and contributes in the development of roots and leaves crucial for drought resistance.

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“…Previously, Arabidopsis 14-3-3 λ was known to interact with a putative peroxisomal ascorbate peroxidase APX3, which is an enzyme that scavenges H 2 O 2 to prevent cell damage from this reactive oxygen species, and AKR2, an ankyrin repeat-containing protein that is probably involved in antioxidation metabolism (Yan et al, 2002). In addition, it was shown that this 14-3-3 protein interacted with SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KIN-ASE (SERK1) (Rienties et al, 2005). SERK1 is a very important component in brassinosteroid signaling in combination with BAK1, another receptor-like protein kinase, which plays a critical role in PTI induction in Arabidopsis with other PRRs such as FLS2 and EFR (Zipfel, 2008).…”

Section: Involvement Of 14-3-3 Proteins In Plant Immunitymentioning

confidence: 99%

Characteristics of 14-3-3 Proteins and Their Role in Plant Immunity

Oh¹

2010

The Plant Pathology Journal

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The Arabidopsis SERK1 protein interacts with the AAA-ATPase AtCDC48, the 14-3-3 protein GF14λ and the PP2C phosphatase KAPP

Cited by 63 publications

References 44 publications

Nucleocytoplasmic Shuttling of BZR1 Mediated by Phosphorylation Is Essential inArabidopsisBrassinosteroid Signaling

Nucleocytoplasmic Shuttling of BZR1 Mediated by Phosphorylation Is Essential inArabidopsisBrassinosteroid Signaling

Physiological and Mechanical Role of 14-3-3 Lambda in Arabidopsis thaliana during Drought Stress

Characteristics of 14-3-3 Proteins and Their Role in Plant Immunity

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