The application of whole-cell protein electrophoresis for the classification and identification of basidiomycetous yeast species

Abstract

The relationships among 65 basidiomycetous yeast strains were determined by one-dimensional electrophoresis of SDS-solubilized whole-cell proteins. Protein profiles were compared by the Pearson product moment correlation coefficient (r). The strains investigated represented species from the genera Cystofilobasidium, Filobasidium, Filobasidiella, Kondoa, Leucosporidium, Mrakia and Rhodosporidium. Except for the genus Mrakia, all species constituted separate protein electrophoretic clusters. The species of the genus Mrakia (M. frigida, M. gelida, M. nivalis and M. stokesii) show highly similar protein patterns, suggesting that these four species may be synonymous. Strains of two varieties of Filobasidiella neoformans, F. neoformans var. neoformans and F. neoformans var. bacillispora, could not be differentiated by protein electrophoresis. For the delineation of the protein electrophoretic clusters of the yeasts studied, literature data relying on other criteria, such as DNA base composition, carbon source utilization patterns, enzymatic protein electrophoregrams, ubiquinone systems, DNA-DNA homology and rRNA sequence data were used. It was demonstrated that a database of SDS-protein patterns provides a valuable tool for the identification of yeasts.

Allergens from Fusarium solani Identified by Immunoblotting in Asthma Patients In Iran

“…Polyacrylamide gel electrophoresis is frequently used to isolate and identify proteins from different fungal species (21). The same method was successfully employed in the present study.…”

Analysis of parity between protein-based electrophoretic methods for the characterization of oral Candida species

“…The analysis of electrophoretic profiles of proteins and multilocus-enzymes has allowed the identification, classification of numerous strains, species and genera of yeasts (Baptist & Kurtzman 1976, Okunishi et al 1979, Yamazaki & Komagata 1981, Maiden & Tanner 1991, Vancanneyt et al 1991, 1992.…”

“…Different types of electrophoretic techniques have been used for the characterization or typing of Candida including electrophoretic separation of chromosomes (Monod et al 1990, Asakura et al 1991, DNA fragments (Scherer & Stevens 1987), multilocus-enzymes (Lehmann et al 1989a, Pujol et al 1993, Reynes et al 1996, and whole-cell proteins (Shen et al 1988, Vancanneyt et al 1991, 1992, Höfling et al 1998). The two latter methods have been used successfully for yeast characterization.…”

Evaluation of biochemical and serological methods to identify and clustering yeast cells of oral Candida species by CHROMagar test, SDS-PAGE and ELISA

“…Strain (13), initially classified as C. krusei, could not be clustered with any other cluster. Strains could have been incorrectly identified or somehow altered during their culturing, manipulation or storage as has already been mentioned by other authors (Vancanneyt et al, 1991;Vancanneyt et al, 1992). These results may be seen in the CA medium, ELISA, and in the SDS-PAGE methods.…”