Allergens from Fusarium solani Identified by Immunoblotting in Asthma Patients In Iran

Khosravi, Alireza; Fatahinia, Mahnaz; Shokri, Hojjatollah; Yadegari, Mohammad Hossein

doi:10.2478/10004-1254-63-2012-2155

Cited by 13 publications

(7 citation statements)

References 24 publications

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“…Significant taxonomic differences in mycobiome were detected between untreated asthma group and controls, 10 genera were enriched in untreated asthma group relative to CON group: Wallemia, Mortierella, Fusarium, unclassified_f_Chaetomiaceae, Phialophora, Metarhizium, unclassified_f_Sporormiaceae, Irpex, Schizophyllum, Rhodotorula. This is consistent with pioneering research on several of them, which has been suggested to be associated with asthma [15, 26 ]. We also noted reductions of well‐known pathogens (Wallemia, Alternaria and Aspergillus) in ICS asthma group relative to untreated asthma group, supporting the idea that the ICS treatment can influence airway mycobiome.…”

Section: Discussionsupporting

confidence: 91%

Fungal and bacterial microbiome dysbiosis and imbalance of trans-kingdom network in asthma

Huang

et al. 2020

Clin Transl Allergy

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Background Fungal and bacterial microbiota play an important role in development of asthma. We aim to characterize airway microbiome (mycobiome, bacteriome) and functional genes in asthmatics and controls. Methods Sputum microbiome of controls, untreated asthma patients and inhaled corticosteroid (ICS) receiving patients was detected using high throughput sequencing. Metagenomic sequencing was used to examine the functional genes of microbiome. Results 1. Mycobiome: α diversity was lower in untreated asthma group than that in controls. Mycobiome compositions differed among the three groups. Compared with controls, untreated asthma group has higher abundance of Wallemia, Mortierella and Fusarium. Compared with untreated asthma patients, ICS receiving patients has higher abundance of Fusarium and Mortierella, lower frequency of Wallemia, Alternaria and Aspergillus. 2. Bacteriome: α diversity was lower in untreated asthma group than that in controls. There are some overlaps of bacteriome compositions between controls and untreated asthma patients which were distinct from ICS receiving patients. Untreated asthma group has higher Streptococcus than controls. 3. Potential fungal and bacterial biomarkers of asthma: Trametes, Aspergillus, Streptococcus, Gemella, Neisseria, etc. 4. Correlation network: There are dense and homogenous correlations in controls but a dramatically unbalanced network in untreated asthma and ICS receiving patients, which suggested the existence of disease-specific inter-kingdom and intra-kingdom alterations. 5. Metagenomic analysis: functional pathways were associated with the status of asthma, microbiome and functional genes showed different correlations in different environment. Conclusion We showed mycobiome and bacteriome dysbiosis in asthma featured by alterations in biodiversity, community composition, inter-kingdom and intra-kingdom network. We also observed several functional genes associated with asthma.

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Section: Discussionsupporting

confidence: 91%

Fungal and bacterial microbiome dysbiosis and imbalance of trans-kingdom network in asthma

Huang

et al. 2020

Clin Transl Allergy

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“…63 Trichoderma and Fusarium species sensitization has also been demonstrated in patients with asthma, atopy, and allergic rhinitis. 64,65 The presence of these fungal genera in the airway microbiota in select asthma phenotypes, coupled with these associations, suggests either a colonizing or a potential pathogenic role. Their presence in select phenotypes offers the possibility that they might be useful, either alone or as part of a cluster of other biological or clinical markers, to delineate clinical asthma endotypes.…”

Section: Discussionmentioning

confidence: 99%

Associations between fungal and bacterial microbiota of airways and asthma endotypes

Sharma

Laxman

Naureckas

et al. 2019

Journal of Allergy and Clinical Immunology

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Background: The relationship between asthma, atopy, and underlying type 2 (T2) airway inflammation is complex. Although the bacterial airway microbiota is known to differ in asthmatic patients, the fungal and bacterial markers that discriminate T2-high (eosinophilic) and T2-low (neutrophilic/ mixed-inflammation) asthma and atopy are still incompletely identified. Objectives: The aim of this study was to demonstrate the fungal microbiota structure of airways in asthmatic patients associated with T2 inflammation, atopy, and key clinical parameters. Methods: We collected endobronchial brush (EB) and bronchoalveolar lavage (BAL) samples from 39 asthmatic patients and 19 healthy subjects followed by 16S gene and internal transcribed spacer-based microbiota sequencing. The microbial sequences were classified into exact sequence variants. The T2 phenotype was defined by using a blood eosinophil count with a threshold of 300 cells/mL. Results: Fungal diversity was significantly lower in EB samples from patients with T2-high compared with T2-low inflammation; key fungal genera enriched in patients with T2high inflammation included Trichoderma species, whereas Penicillium species was enriched in patients with atopy. In BAL fluid samples the dominant genera were Cladosporium, Fusarium, Aspergillus, and Alternaria. Using generalized linear

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“…The 11 kDa Fus c 1 (60S acidic ribosomal protein P2), 13 kDa Fus c 2 (thioredoxin-like protein) and 49 kDa Fus c 3 (not related to known proteins) have IgE-binding frequencies of 35, 50, and 15%, respectively, with sera from 26 individuals sensitized to F. culmorum [13]. Recently, Khosravi et al in Iran showed with immunoblotting that F. solani has six major allergens with molecular masses of 24, 58.5, 64.5, 69, 72 and 97 kDa [27]. In this study, we did not detect a major IgE-binding F. proliferatum protein with molecular mass of about 65 kDa.…”

Section: Discussionmentioning

confidence: 99%

The Transaldolase, a Novel Allergen of Fusarium proliferatum, Demonstrates IgE Cross-Reactivity with Its Human Analogue

Chou

Yeh

et al. 2014

PLoS ONE

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Fusarium species are among airborne fungi and recognized as causative agents of human atopic disorders. However, Fusarium allergens have not been well characterized and the lack of information limits clinical diagnosis and treatment of fungal allergy. The purpose of this study is to identify and characterize important allergens of F. proliferatum. IgE-reacting F. proliferatum components were identified by immunoblot using serum samples from patients of respiratory atopic diseases. Characterization of allergens and determination of IgE cross-reactivity were performed by cDNA cloning, then homologous expression and immunoblot inhibition studies. We identified nine different F. proliferatum components that can be recognized by IgE antibodies in 17 (28%) of the 60 atopic sera tested. Components with molecular masses of about 43, 37.5 and 36.5 kDa with IgE-binding frequencies of about 88, 47 and 53%, respectively, were considered as important allergens of F. proliferatum. The 37.5 kDa IgE-binding component was putatively considered as a transaldolase protein of F. proliferatum. The full-length cDNA of F. proliferatum transaldolase was subsequently cloned. It encodes an open reading frame of 312 amino acids and has sequence identifies of 73 and 61%, respectively, with Cladosporium and human transaldolases. The purified recombinant F. proliferatum transaldolase can inhibit the IgE-binding against the 37.5 kDa component of F. proliferatum and the transaldolase allergen from Cladosporium cladosporioides. More importantly, the recombinant F. proliferatum transaldolase can inhibit IgE-binding against human transaldolase in a concentration-dependent manner. Thus, a novel and important F. proliferatum transaldolase allergen was identified. In addition to IgE cross-reactivity between the Fusarium and the Cladosporium transaldolase allergens, IgE cross-reactivity between the Fusarium and the human transaldolases also exists and might contribute to atopic manifestations in the absence of exogenous allergen exposure.

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Allergens from Fusarium solani Identified by Immunoblotting in Asthma Patients In Iran

Cited by 13 publications

References 24 publications

Fungal and bacterial microbiome dysbiosis and imbalance of trans-kingdom network in asthma

Fungal and bacterial microbiome dysbiosis and imbalance of trans-kingdom network in asthma

Associations between fungal and bacterial microbiota of airways and asthma endotypes

The Transaldolase, a Novel Allergen of Fusarium proliferatum, Demonstrates IgE Cross-Reactivity with Its Human Analogue

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