The application of proteomic methods (MALDI-toff MS) for studying protein profiles of some nematodes (dirofilaria and ascaris) for differentiating species

Nagorny, Sergey Andreevich; Aleshukina, A.; Aleshukina, I.; Ermakova, L.; Pshenichnaya, N.

doi:10.1016/j.ijid.2019.02.047

Cited by 12 publications

(11 citation statements)

References 18 publications

(27 reference statements)

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“…As an example, Bruker has developed the MBT Sepsityper IVD Kit for the identification of microorganisms from blood cultures using a MALDI–TOF MS platform [ 18 , 19 , 20 ]. Interestingly, a recent article reported the use of the MBT Sepsityper Kit in order to profile four nematodes ( Dirofilaria repens , Dirofilaria immitis , Ascaris suum , and Ascaris lumbricoides ) [ 10 ], which intrigued and prompted us to test this method on Anisakis spp. In fact, our future goal, that has driven the preliminary study here presented, is to develop a pipeline for a proteomic profiling of Anisakis spp.…”

Section: Discussionmentioning

confidence: 99%

“…However, an increase of applications of this technology has been observed in the last 10 years, with encouraging results also in studies regarding parasites [ 8 , 9 ]. A recent application of MALDI–TOF MS for Dirofilaria and Ascaris protein-based profiling showed a promising scenario towards clinical applications involving nematodes biotyping [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Mass Spectrometry Based-Proteomic Analysis of Anisakis spp.: A Preliminary Study towards a New Diagnostic Tool

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Foglietta

et al. 2020

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Anisakiasis is nowadays a well-known infection, mainly caused by the accidental ingestion of Anisakis larvae, following the consumption of raw or undercooked fishes and cephalopods. Due to the similarity of symptoms with those of common gastrointestinal disorders, this infection is often underestimated, and the need for new specific diagnostic tools is becoming crucial. Given the remarkable impact that MALDI–TOF MS biotyping had in the last decade in clinical routine practice for the recognition of bacterial and fungi strains, a similar scenario could be foreseen for the identification of parasites, such as nematodes. In this work, a MALDI–TOF MS profiling of Anisakis proteome was pursued with a view to constructing a first spectral library for the diagnosis of Anisakis infections. At the same time, a shotgun proteomics approach by LC–ESI–MS/MS was performed on the two main fractions obtained from protein extraction, to evaluate the protein species enriched by the protocol. A set of MALDI–TOF MS signals associated with proteins originating in the ribosomal fraction of the nematode extract was selected as a potential diagnostic tool for the identification of Anisakis spp.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mass Spectrometry Based-Proteomic Analysis of Anisakis spp.: A Preliminary Study towards a New Diagnostic Tool

Marzano

Pane

Foglietta

et al. 2020

Genes

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“…However, data were limited regarding the origin of the study samples, the quality of the spectra obtained by MALDI-TOF and the repeatability of the results. Yet, during the revision of this systematic review, Pshenichnaya et al [145] published their work on dirofilariasis in a peer-reviewed journal and provided also data for two different species of Ascaris (i.e. A. suum and A. lumbricoides ).…”

Section: Resultsmentioning

confidence: 99%

MALDI-TOF mass spectrometry as a diagnostic tool in human and veterinary helminthology: a systematic review

et al. 2019

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Background Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has become a widely used technique for the rapid and accurate identification of bacteria, mycobacteria and certain fungal pathogens in the clinical microbiology laboratory. Thus far, only few attempts have been made to apply the technique in clinical parasitology, particularly regarding helminth identification. Methods We systematically reviewed the scientific literature on studies pertaining to MALDI-TOF MS as a diagnostic technique for helminths (cestodes, nematodes and trematodes) of medical and veterinary importance. Readily available electronic databases (i.e. PubMed/MEDLINE, ScienceDirect, Cochrane Library, Web of Science and Google Scholar) were searched from inception to 10 October 2018, without restriction on year of publication or language. The titles and abstracts of studies were screened for eligibility by two independent reviewers. Relevant articles were read in full and included in the systematic review. Results A total of 84 peer-reviewed articles were considered for the final analysis. Most papers reported on the application of MALDI-TOF for the study of Caenorhabditis elegans , and the technique was primarily used for identification of specific proteins rather than entire pathogens. Since 2015, a small number of studies documented the successful use of MALDI-TOF MS for species-specific identification of nematodes of human and veterinary importance, such as Trichinella spp. and Dirofilaria spp. However, the quality of available data and the number of examined helminth samples was low. Conclusions Data on the use of MALDI-TOF MS for the diagnosis of helminths are scarce, but recent evidence suggests a potential role for a reliable identification of nematodes. Future research should explore the diagnostic accuracy of MALDI-TOF MS for identification of (i) adult helminths, larvae and eggs shed in faecal samples; and (ii) helminth-related proteins that are detectable in serum or body fluids of infected individuals. Electronic supplementary material The online version of this article (10.1186/s13071-019-3493-9) contains supplementary material, which is available to authorized users.

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“…The protein expression profiles determined by 2-DE coupled with MALDI-TOF/TOF MS method were about three times higher than those obtained with 2-DE [ 109 ]. Analysis of only head ends of 10 immature A. lumbricoides and A. suum using MALDI-TOF MS was also performed, but only to describe protein profiles (characteristic protein peaks) of those species [ 110 ]. Most of the proteomic studies were performed on A. suum .…”

Section: Discovery Approach—description Of the Selected Fbps And Tmentioning

confidence: 99%

Proteomic Insights into the Biology of the Most Important Foodborne Parasites in Europe

Stryiński

Łopieńska–Biernat

Carrera

2020

Foods

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Foodborne parasitoses compared with bacterial and viral-caused diseases seem to be neglected, and their unrecognition is a serious issue. Parasitic diseases transmitted by food are currently becoming more common. Constantly changing eating habits, new culinary trends, and easier access to food make foodborne parasites’ transmission effortless, and the increase in the diagnosis of foodborne parasitic diseases in noted worldwide. This work presents the applications of numerous proteomic methods into the studies on foodborne parasites and their possible use in targeted diagnostics. Potential directions for the future are also provided.

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The application of proteomic methods (MALDI-toff MS) for studying protein profiles of some nematodes (dirofilaria and ascaris) for differentiating species

Cited by 12 publications

References 18 publications

Mass Spectrometry Based-Proteomic Analysis of Anisakis spp.: A Preliminary Study towards a New Diagnostic Tool

Mass Spectrometry Based-Proteomic Analysis of Anisakis spp.: A Preliminary Study towards a New Diagnostic Tool

MALDI-TOF mass spectrometry as a diagnostic tool in human and veterinary helminthology: a systematic review

Proteomic Insights into the Biology of the Most Important Foodborne Parasites in Europe

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