The application of novel magnetically coupled mixer drives in bioreactors of up to 15 m3

Šuleiko, Artūrs; Vanags, Juris; Koņuhova, Marina; Dubencovs, Konstantīns; Grīgs, Oskars

doi:10.1016/j.bej.2019.107464

Cited by 6 publications

(7 citation statements)

References 22 publications

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“…Increasing demands on sterility and process safety favor magnetically coupled systems, which are the subject of increased research. Therefore, the basic torque limitation problem of magnetically driven systems should be minimized by progressing technologies and using neodymium magnets (Suleiko et al 2020 ), which are the strongest permanent magnets currently available on the market. The growing trend in recent years to use magnetically driven systems in more complex mixing processes, such as in microbial and cell culture applications (ZETA GmbH n.d.), has also accelerated development up to current scales of 30–40 m 3 (MAVAG AG 2020 ; ZETA GmbH 2020a ).…”

Section: Discussionmentioning

confidence: 99%

“…However, technical progress has also recently been made in terms of the use of magnetic couplings in larger bioprocesses. With their adapted magnetic system, Suleiko et al ( 2020 ) demonstrated a drive with a torque of 200 Nm that is suitable for biological applications at a scale of 15 m 3 . ZETA’s magnetic bottom-mounted agitator for bioreactors with a torque of more than 400 Nm was also evaluated at the same scale (ZETA GmbH n.d. ; Rosseburg et al 2018 ; Fitschen et al 2019 ), delivering results that predict applicability at scales up to 30 m 3 (ZETA GmbH 2020a ).…”

Section: Drive Systems and Seals Commonly Used In Biotechnological Prmentioning

confidence: 99%

“…Furthermore, these magnets often include rare earth materials (Dickey 2015 ). The field of application of such systems is defined by the temperature resistance of the magnets, which means that this type of coupling is mainly implemented for processes operating at moderate temperatures and sterilization processes at temperatures of up to 120 °C (Suleiko et al 2020 ). This is due to the deterioration of ferromagnetic properties at high temperatures.…”

Section: Drive Systems and Seals Commonly Used In Biotechnological Prmentioning

confidence: 99%

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An overview of drive systems and sealing types in stirred bioreactors used in biotechnological processes

Schirmer

Maschke

Pörtner

et al. 2021

Appl Microbiol Biotechnol

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No matter the scale, stirred tank bioreactors are the most commonly used systems in biotechnological production processes. Single-use and reusable systems are supplied by several manufacturers. The type, size, and number of impellers used in these systems have a significant influence on the characteristics and designs of bioreactors. Depending on the desired application, classic shaft-driven systems, bearing-mounted drives, or stirring elements that levitate freely in the vessel may be employed. In systems with drive shafts, process hygiene requirements also affect the type of seal used. For sensitive processes with high hygienic requirements, magnetic-driven stirring systems, which have been the focus of much research in recent years, are recommended. This review provides the reader with an overview of the most common agitation and seal types implemented in stirred bioreactor systems, highlights their advantages and disadvantages, and explains their possible fields of application. Special attention is paid to the development of magnetically driven agitators, which are widely used in reusable systems and are also becoming more and more important in their single-use counterparts.Key Points• Basic design of the most frequently used bioreactor type: the stirred tank bioreactor• Differences in most common seal types in stirred systems and fields of application• Comprehensive overview of commercially available bioreactor seal types• Increased use of magnetically driven agitation systems in single-use bioreactors

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Section: Discussionmentioning

confidence: 99%

Section: Drive Systems and Seals Commonly Used In Biotechnological Prmentioning

confidence: 99%

Section: Drive Systems and Seals Commonly Used In Biotechnological Prmentioning

confidence: 99%

See 1 more Smart Citation

An overview of drive systems and sealing types in stirred bioreactors used in biotechnological processes

Schirmer

Maschke

Pörtner

et al. 2021

Appl Microbiol Biotechnol

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“…where P o -Power Number; P MAX -maximal power, W; R-agitation rate, rps; d-diameter of the external rotor, m. The impeller power number (0.55), which was used for the observed calculation, was estimated using the methods described in previously performed studies [16]. The impeller diameter was equal to 80 mm.…”

Section: Mechanical Cell Susceptibility Assessmentmentioning

confidence: 99%

Investigation of Crypthecodinium cohnii High-Cell-Density Fed-Batch Cultivations

Dubencovs,

Suleiko,

Suleiko

et al. 2024

Fermentation

Self Cite

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Crypthecodinium cohnii is a marine microalga that can accumulate high amounts of polyunsaturated fatty acids (PUFAs) and thus replace conventional routes of fish oil production. They are associated with the destruction of marine resources and multiple downstream/purification complications. The major drawbacks of using C. cohnii for industrial-scale production are associated with low PUFA productivity. One of the means of increasing the PUFA synthesis rate is to maintain the medium component concentrations at optimal values throughout cultivation, thus increasing PUFA production efficiency, which can result in the successful transfer of the process to pilot and/or industrial scale. The goal of the present research was to develop techniques for increasing the efficiency of PUFA production via C. cohnii cultivation. Multiple experiments were carried out to test and fine-tune the cultivation medium composition and oxygen transfer factors. The biomass yields from individual components, yeast extract, sea salts, and glucose amounted to 5.5, 0.65, and 0.61 g·g−1, respectively. C. cohnii cell susceptibility to mechanical damage was experimentally evaluated. Power inputs of <276.5 W/m3 did not seem to promote cell destruction when Pitched-blade impellers were used. The obtained cultivation conditions were shown to be efficient in terms of increasing the biomass productivity and the omega-3 fatty acid content in C. cohnii. By using the applied methods, the maximal biomass productivity reached 8.0 g·L−1·day−1, while the highest obtained biomass concentration reached 110 g·L−1. A steady increase in the concentration of PUFAs during cultivation was observed from the FTIR data.

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“…[64,237,16] CYP450 enzymes catalyze the oxidation of many compounds; therefore, stable and sufficient levels of CYP450 isoenzymes must be provided to hepatocytes in liver on chips. [254] Ramli et al used real-time imaging assays with fluorescent compounds (5-carboxy-2, 7-dichlorofluorescein) to visualize the biliary tubular network and bile secretion functions of liver organoids.…”

Section: Key Indices For Partial Organ Functionmentioning

confidence: 99%

Microphysiological Constructs and Systems: Biofabrication Tactics, Biomimetic Evaluation Approaches, and Biomedical Applications

Zhang,

Xu,

et al. 2023

Small Methods

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In recent decades, microphysiological constructs and systems (MPCs and MPSs) have undergone significant development, ranging from self‐organized organoids to high‐throughput organ‐on‐a‐chip platforms. Advances in biomaterials, bioinks, 3D bioprinting, micro/nanofabrication, and sensor technologies have contributed to diverse and innovative biofabrication tactics. MPCs and MPSs, particularly tissue chips relevant to absorption, distribution, metabolism, excretion, and toxicity, have demonstrated potential as precise, efficient, and economical alternatives to animal models for drug discovery and personalized medicine. However, current approaches mainly focus on the in vitro recapitulation of the human anatomical structure and physiological‐biochemical indices at a single or a few simple levels. This review highlights the recent remarkable progress in MPC and MPS models and their applications. The challenges that must be addressed to assess the reliability, quantify the techniques, and utilize the fidelity of the models are also discussed.

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The application of novel magnetically coupled mixer drives in bioreactors of up to 15 m3

Cited by 6 publications

References 22 publications

An overview of drive systems and sealing types in stirred bioreactors used in biotechnological processes

An overview of drive systems and sealing types in stirred bioreactors used in biotechnological processes

Investigation of Crypthecodinium cohnii High-Cell-Density Fed-Batch Cultivations

Microphysiological Constructs and Systems: Biofabrication Tactics, Biomimetic Evaluation Approaches, and Biomedical Applications

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The application of novel magnetically coupled mixer drives in bioreactors of up to 15 m3

Cited by 6 publications

References 22 publications

An overview of drive systems and sealing types in stirred bioreactors used in biotechnological processes

An overview of drive systems and sealing types in stirred bioreactors used in biotechnological processes

Investigation of Crypthecodinium cohnii High-Cell-Density Fed-Batch Cultivations

Microphysiological Constructs and Systems: Biofabrication Tactics, Biomimetic Evaluation Approaches, and Biomedical Applications

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The application of novel magnetically coupled mixer drives in bioreactors of up to 15 m3