The application of microsatellite analysis in barley malting quality breeding programmes

Abstract

Leišová-Svobodová L., Tomková L., Sedláček T., Psota V., Kučera L. (2014): The application of microsatellite analysis in barley malting quality breeding programmes. Czech J. Genet. Plant Breed., 50: 268-277.A set of 43 microsatellite loci was used to characterise the malting barley varieties and breeding lines used for the Protected Geographical Indication (PGI) "České pivo" ("Czech beer") and for other beer types. Genotype data were compared with technological malting quality parameters. The analysis of variance showed significant differences between varieties used for the given PGI and varieties used for other beer types in six variables. Three clusters of varieties specific to the PGI and three clusters specific to malting barley genotypes for other beer types were identified using a Bayesian approach. The remaining four clusters expressed effects of parent lines. The discrimination analysis based on malting quality and molecular variables identified only one barley breeding line suitable for the PGI "České pivo"".

Comparative de novo transcriptome analysis of barley varieties with different malting qualities

“…6 ). It contributes to higher level of proteolytic and cytolytic modifications, resulting in the significantly lower level of residual extracts in the final product as was found in the previous study (Leišová-Svobodová et al 2014 ).

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“…The study proceeded from the assumption that the heritability for malting traits is high - it ranges from 0.50 to 0.98 (Schmidt et al 2016 ) and therefore genetic differences between the C and E barley groups with different malting qualities (Leišová-Svobodová et al 2014 ) were expected. Barley transcripts derived from three barley malting stages were de novo assembled from Illumina reads.…”

“…Eight barley varieties were selected according to technological parameters of malting quality (Leišová-Svobodová et al 2014 ). A group of four barley varieties (Aksamit, Bojos, Malz, and Blaník), which is recommended for the production of PGI “České pivo” beer, further labeled as C group and a group of four varieties (Sebastian, Kangoo, Xanadu, and Zeppelin), which have very good malting qualities but are not recommended for the production of PGI “České pivo” beer, further labeled as E group.…”