The Application of JPEG2000 in Virtual Microscopy

Tuominen, Vilppu J.; Isola, Jorma

doi:10.1007/s10278-007-9090-z

Cited by 64 publications

(63 citation statements)

References 13 publications

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“…Sections were visualized with an Olympus BX51 microscope and Olympus ColorView IIIu camera using a ϫ100 magnification or with a fully automated Objective Imaging Surveyor virtual slide scanner (Objective Imaging, Cambridge, United Kingdom). Digitization of scanned sample sections was done at a resolution of 0.4 m per pixel using a 20ϫ Plan Apochromatic microscope objective, and image data were converted to JPEG2000 format as described previously (35).…”

Section: Mo and M Marinum Coinjectionsmentioning

confidence: 99%

The Proprotein Convertase Subtilisin/Kexin FurinA Regulates Zebrafish Host Response against Mycobacterium marinum

et al. 2015

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Tuberculosis is a chronic bacterial disease with a complex pathogenesis. An effective immunity against Mycobacterium tuberculosis requires both the innate and adaptive immune responses, including proper T helper (Th) type 1 cell function. FURIN is a proprotein convertase subtilisin/kexin (PCSK) enzyme, which is highly expressed in Th1 type cells. FURIN expression in T cells is essential for maintaining peripheral immune tolerance, but its role in the innate immunity and infections has remained elusive. Here, we utilized Mycobacterium marinum infection models in zebrafish (Danio rerio) to investigate how furin regulates host responses against mycobacteria. In steady-state furinA td204e/؉ fish reduced furinA mRNA levels associated with low granulocyte counts and elevated Th cell transcription factor expressions. Silencing furin genes reduced the survival of M. marinuminfected zebrafish embryos. A mycobacterial infection upregulated furinA in adult zebrafish, and infected furinA td204e/؉ mutants exhibited a proinflammatory phenotype characterized by elevated tumor necrosis factor a (tnfa), lymphotoxin alpha (lta) and interleukin 17a/f3 (il17a/f3) expression levels. The enhanced innate immune response in the furinA td204e/؉ mutants correlated with a significantly decreased bacterial burden in a chronic M. marinum infection model. Our data show that upregulated furinA expression can serve as a marker for mycobacterial disease, since it inhibits early host responses and consequently promotes bacterial growth in a chronic infection.T uberculosis (TB) is an epidemic infectious disease caused by the mycobacterial species Mycobacterium tuberculosis (1, 2). Circa 13% of the individuals with active TB were simultaneous carriers of the human immunodeficiency virus (HIV), and almost one-third of TB-associated deaths occurred among HIV ϩ patients, demonstrating the critical role of cluster of differentiation 4 (CD4 ϩ ) T lymphocyte-mediated immunity in the control of M. tuberculosis infection (3, 4). More specifically, the adaptive immunity against TB is primarily mediated by T helper (Th) type 1 cells, as is suggested by the gene expression profile upon infection (5), as well as the infection-induced mortality of gamma interferon-deficient (6, 7) and interleukin-12 (IL-12)-deficient (8) mice.The proprotein convertase subtilisin/kexin (PCSK) enzymes are a family of serine endoproteases with nine members in humans: PCSK1 and -2, FURIN, PCSK4 to -7, membrane-bound transcription factor peptidase site 1 (MBTPS1), and PCSK9 (9). Typically, PCSKs convert precursor proteins (proproteins) into their biologically active forms by cleaving them at specific target motifs made up of the basic amino acids lysine and arginine (9, 10). FURIN was the first identified mammalian PCSK and is present in vertebrates and many invertebrates (11,12). A series of in vitro experiments have suggested a central role for FURIN in host defense because it proteolytically activates several immunoregulatory proproteins, such as membrane-inserted matrix metallo...

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Section: Mo and M Marinum Coinjectionsmentioning

confidence: 99%

The Proprotein Convertase Subtilisin/Kexin FurinA Regulates Zebrafish Host Response against Mycobacterium marinum

et al. 2015

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“…Customised formats are an inconvenience to end users, as this requires installing dedicated browsers, which is a major obstacle in the digital microscopy market. It is therefore proposed that formats should be standardised to JPEG 2000 [22].…”

Section: It Infrastructurementioning

confidence: 99%

Future perspectives of digital pathology

Prochorec‐Sobieszek

2016

Nowotwory. Journal of Oncology

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Technological advances have enabled innovative solutions to be achieved in pathology based on digital imaging, now superseding those of conventional microscopy. Digital pathology has been defined as 'virtual microscopy' and depends on computer-generated digital imaging of microscope slides (WSI -whole slide imaging) which are in turn created, reviewed, managed, shared, analysed and interpreted. Such WSI systems and digital consulting platforms are now used for teaching, scientific research, telepathology / teleconsultation and diagnostics. They also permit easy and interactive sharing of WSI that can be integrated into other medical information systems. The software for automated image analysis and computer aided diagnosis can thereby make highly accurate diagnoses and help standardise study findings. Despite the technique's many advantages, its noted drawbacks include high equipment and software costs, image quality issues of standardisation and most importantly, that pathologists are reluctant to use it routinely for making diagnoses. NOWOTWORY J Oncol 2016; 66, 4: 277-285

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“…Die Vorteile von JPEG2000 gegenüber JPEG sind bereits in vorangegangenen Publikationen beschrieben worden [6][7][8][9][10][11]. Tuominen u. Isola [8] haben die höhere Leistungsfähigkeit eines JPEG2000-Servers im direkten Vergleich zu einem JPEG-basierten System (Zoomify) nachgewiesen. Dabei erlaubt der JPEG2000-Server bei gleicher technischer Ausstattung einer wesentlich höheren Anzahl an Nutzern das simultane Mikroskopieren des gleichen virtuellen Präparats.…”

Section: Diskussionunclassified

Pathowiki

Roßner

Zwönitzer³

et al. 2012

Pathologe

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The project Pathowiki (www.pathowiki.org) is a free expert database for texts, images, virtual slides and links to all subject areas of pathology in the internet. The aim of this project is to integrate all available information and media, in particular virtual microscopy, to achieve a fast overview of a relevant subject area. Here we present the project’s basic functions and applications and evaluate the project with respect to the ongoing digital developments in pathology.

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The Application of JPEG2000 in Virtual Microscopy

Cited by 64 publications

References 13 publications

The Proprotein Convertase Subtilisin/Kexin FurinA Regulates Zebrafish Host Response against Mycobacterium marinum

The Proprotein Convertase Subtilisin/Kexin FurinA Regulates Zebrafish Host Response against Mycobacterium marinum

Future perspectives of digital pathology

Pathowiki

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