In regard to the functional system or process, there is always at least one constraint; and some advanced techniques, that use a focusing process to identify this constraint and restructure the rest of the organization around it, may be implemented. The paper deals with the application of the specific Theory of Constraints tool in given logistics operation within particular manufacturing and distribution enterprise. The objective of this utilization is to define the most significant cause in terms of determined negative consequences. The relevant effect on cost value, effectivity and profitability as well as quality is the crucial reason to detect a particular cause resulting in negative consequences. The selected enterprise specifies seven main consequences and due to the construction of the special diagram of causes and consequences (as a part of the Theory of Constraints tools), it was possible to determine the most important cause.