The Application of Fe-EDTA and Sodium Silicate Affects the Polyphenols Content in Broccoli and Radish Sprouts

Abstract

The effects of elicitors on broccoli (Brassica oleracea L. var. Italica) and radish (Raphanus sativus L.) sprouts were evaluated. Seeds and then sprouts were soaked daily for 30 min over 6 days in water (control) or a mixture of FeEDTA and sodium silicate or sodium silicate alone. The contents of the flavonoids and phenolic acids (free, esters, and glycosides) were determined using HPLC-ESI-MS/MS. Phenolic compounds were released from the esters after acid hydrolysis and from the glycosides using alkaline hydrolysis. Quercetin, kaempferol, (‒)-epicatechin, naringenin, apigenin, and luteolin derivatives were found in broccoli and radish sprouts, while derivatives of iso-rhamnetin, orientin, and vitexin were not present at measurable levels. The flavonoid contents, especially derivatives of quercetin, were considerably higher in the broccoli sprouts than in the radish sprouts. The quantitatively major phenolic acid content in the sprouts of both species was found to be p-hydroxybenzoic acid. Its content in the radish sprouts was several times higher than in the broccoli sprouts. The total flavonoid content of broccoli sprouts was 507–734 µg/g DW, while that of the radish sprouts ranged from 155 µg/g DW to 211 µg/g DW. In contrast, total phenolic acids were higher in radish sprouts, ranging from 11,548 to 13,789 µg/g DW, while in broccoli sprouts, they ranged from 2652 to 4527 µg/g DW, respectively. These differences resulted radish sprouts having higher antioxidant activity compared to broccoli sprouts. The applied elicitors increased the content of the total phenolic acids and the antioxidant activity of radish and broccoli sprouts, while they decreased the level of the total flavonoids in broccoli sprouts.

In Vitro Evidence on Bioaccessibility of Flavonols and Cinnamoyl Derivatives of Cruciferous Sprouts

“…However, the studied sprouts exhibited significantly different concentrations of total (poly)phenols, as follows: broccoli (137.88 mg/g fresh weight (fw)) > white mustard (120.95 mg/g fw) > red cabbage (108.03 ng/g fw) > red radish (64.25 mg/g fw) (Figure 2). These concentrations are in good agreement with previous descriptions in the literature [18][19][20]. However, in order to understand the biological interest of cruciferous sprouts' phenolics, the analysis of bioaccessibility needs to be addressed.…”

Impact of Sodium Silicate Supplemented, IR-Treated Panax Ginseng on Extraction Optimization for Enhanced Anti-Tyrosinase and Antioxidant Activity: A Response Surface Methodology (RSM) Approach

“…Sodium silicate 1.0 mM, 2 h 60 • C in aerial showed the best results, whereas sodium silicate 1.0 mM 1 h 40 • C was the best for media treatment in sodium silicate-treated extracts with the content of 1183.52 ± 23.02 and 1017.42 ± 4.605 mg GAE/100 g, respectively. It was previously reported that the sodium silicate treatment enhances the total phenolic content when treated with Fe-EDTA and sodium [47,48]. Overall, phenolic content remained stable across different temperatures and time points.…”

Wheat sprouts (Triticum aestivum Linn.) cultured by a smart farm system ameliorate NAFLD through the AMPK-mediated SREBP signaling pathway

“…This bioprocess also enhances vitamin and bioactive compound levels. Common seeds used for sprouts include alfalfa (Logan et al, 2001 ; Zhang et al, 2022 ), broccoli (Chiriac et al, 2020 ; Subedi et al, 2019 ), clover (Dębski et al, 2021 ; Zhang et al, 2022 ), radishes (Subedi et al, 2019 ), rapeseed (Galanty et al, 2022 ), Chinese cabbage, red cabbage, barley, and wheat (Xiao et al, 2022 ). However, the contents may differ depending on the cultivation method of the plant (Galanty et al, 2022 ).…”