The Application of Bacillus subtilis for Adhesion Inhibition of Pseudomonas and Preservation of Fresh Fish

Zhang, Wen; Tong, Qiuxia; You, Jiahong; Lv, Xu-Cong; Liu, Zhibin; Ni, Li

doi:10.3390/foods10123093

Cited by 15 publications

(6 citation statements)

References 38 publications

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“…Mucus was prepared using the method published by Chen et al [14] with slight modification. The adhesion rate was determined according to Zhang et al [12].The adhesion rate was calculated using Equation (1).…”

Section: Determination Of Adhesion In Vitromentioning

confidence: 99%

“…TVB-N was determined following the method described by Zhang et al [12], and calculated using Equation ( 2…”

Section: Determination Of Total Volatile Basic Nitrogen (Tvb-n)mentioning

confidence: 99%

“…Using the extracted fish gut genomic DNA as a template, specific primers (bacterial primers 341-F (50-CCT AYG GGR BGC ASC AG-30) and 806-R (50-GGA CTA CNN GGG TAT CTA AT-30) with barcodes were synthesized according to the V3-V4 region sequences for PCR amplification. The specific steps for high-throughput sequencing were according to Zhang et al [12].…”

Section: Analysis Of Changes Of Intestinal Microflora In Tilapia By H...mentioning

confidence: 99%

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The Adhesion and Spoilage of Shewanella putrefaciens in Tilapia

Zhang

et al. 2022

Foods

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Shewanella putrefaciens is a typical spoilage bacteria organism in seafood. The adhesion ability of three S. putrefaciens strains (HR-15, JR-18, HC-71) isolated from putrefied tilapia were evaluated by mucus adhesion in vitro and intestinal adhesion in vivo. The results of the spoilage of the inoculated fish fillets and spoilage of the refrigerated fish both showed that the adhesion ability of S. putrefaciens was positively correlated with the spoilage ability. High-throughput sequencing and GC-MS results showed that S. putrefaciens with high adhesion ability also significantly changed the intestinal flora of fish, causing an increase in the intestinal bacteria such as Plesionomas, Macellibacteroides, Acinetobacter, and Legionella, which then led to the increase in volatile substances such as low-grade aldehydes, alcohols, and ketones in the fish, serious fatty acid oxidation, and excitement of the fishy smell.

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Section: Determination Of Adhesion In Vitromentioning

confidence: 99%

“…TVB-N was determined following the method described by Zhang et al [12], and calculated using Equation ( 2…”

Section: Determination Of Total Volatile Basic Nitrogen (Tvb-n)mentioning

confidence: 99%

Section: Analysis Of Changes Of Intestinal Microflora In Tilapia By H...mentioning

confidence: 99%

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The Adhesion and Spoilage of Shewanella putrefaciens in Tilapia

Zhang

et al. 2022

Foods

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“…Recent years have highlighted their benefits in aquaculture, notably in enhancing growth performance, disease control, immune response improvement, and digestive enzyme activity [16,17]. Among these, Bacillus species are widely employed as probiotics in aquatic animals [18][19][20][21]. Studies have demonstrated that probiotics do not lead to drug resistance in aquatic species, making them viable antibiotic substitutes [22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Strain-Specific Benefits of Bacillus Probiotics in Hybrid Grouper: Growth Enhancement, Metabolic Health, Immune Modulation, and Vibrio harveyi Resistance

Han,

Song,

Cui

et al. 2024

Animals

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In the realm of modern aquaculture, the utilization of probiotics has gained prominence, primarily due to their ability to enhance growth, boost immunity, and prevent diseases in aquatic species. This study primarily investigates the efficacy of Bacillus subtilis strains, both host-derived and from other sources, in influencing fish growth, immunity, lipid metabolism, and disease resistance. Employing a 42-day feeding trial, we divided hybrid grouper into four distinct groups: a control group on a basal diet and three experimental groups supplemented with 1 × 108 CFU/g of different Bacillus subtilis strains-BS, 6-3-1, and HAINUP40. Remarkably, the study demonstrated that the 6-3-1 and HAINUP40 groups exhibited significant enhancements across key growth parameters: final body weight (FBW), weight gain rate (WGR), feed intake (FI), feed efficiency ratio (FER), and feed conversion ratio (FCR). The investigation into lipid metabolism revealed that the 6-3-1 strain upregulated seven metabolism-related genes, HAINUP40 affected four metabolism-related genes, and the BS strain influenced two metabolism-related genes, indicating diverse metabolic impacts by different strains. Further, a notable reduction in liver enzymes AST and ALT was observed across all supplemented groups, implying improved liver health. Noteworthy was the BS strain’s superior antioxidative capabilities, positively affecting all four measured parameters (CAT, GSH-Px, MDA). In the sphere of immune-related gene expression, the BS strain significantly decreased the expression of both inflammation and apoptosis-related genes, whereas the HAINUP40 strain demonstrated an upregulation in these genes. The challenge test results were particularly telling, showcasing improved survival rates against Vibrio harveyi infection in the BS and 6-3-1 groups, unlike the HAINUP40 group. These outcomes highlight the strain-specific nature of probiotics and their varying mechanisms of action within the host. In conclusion, this study reveals that probiotic strains, varying by source, demonstrate unique, strain-specific effects in promoting growth and modulating immunity in hybrid grouper. This research highlights the promise of tailored probiotic applications in improving aquaculture practices. Such advancements contribute to more sustainable and efficient fish farming methods.

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“…Bacillus subtilis , a well-studied exogenous probiotic in aquaculture, can colonize, grow and reproduce in the intestinal tract of aquatic animals, secreting and producing a variety of digestive enzymes and nutritional metabolites ( Liu et al., 2015 ; Shiu et al., 2015 ; Alan et al., 2016 ; Xu et al., 2016 ; Zuenko et al., 2017 ; Zhang et al, 2021a ). Moreover, its supplementation in diets has a strong beneficial effect on many kinds of fish, and no obvious toxicity has been found with an appropriate amount of supplementation ( Shiu et al., 2015 ; Xu et al., 2016 ; Liu et al., 2018 ; Adelina et al., 2019 ; Ahmadifard et al., 2019 ; Olmos et al., 2020 ; Wang et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%