The Antiviral Efficacy of &amp;lt;i&amp;gt;Withania somnifera&amp;lt;/i&amp;gt; (Ashwagandha) against Hepatitis C Virus Activity: &amp;lt;i&amp;gt;In Vitro&amp;lt;/i&amp;gt; and &amp;lt;i&amp;gt;in Silico&amp;lt;/i&amp;gt; Study

Mofed, Dina; Ahmed, Wafaa; Zekri, Abdel-Rahman N.; Said, Ola; Rahouma, Mohamed; Faraag, Ahmed Hassan Ibrahim

doi:10.4236/aim.2020.109035

Cited by 13 publications

(8 citation statements)

References 30 publications

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“…The viral load reduction as measured by PCR was noted as 6.241 × 103 IU/mL at 25mg/ml concentration. [21] A study on Glycyrrhizin, a major component of Yashtimadhu,…”

Section: Discussionmentioning

confidence: 99%

An In-vitro evaluation of a polyherbal formulation, against SARS-CoV-2

Kanchibhotla¹,

Subramanian²,

reddy³

et al. 2021

Preprint

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Background: COVID-19, caused by SARS-CoV-2 is one of the major health crisis that has affected the world in the past century. With the emergence of new strains of viruses and antimicrobial resistance, the world is looking for an alternate therapeutic option to fight infectious disease.Objective: The present study evaluated the efficacy of a novel polyherbal formulation, named NOQ19, against SARS-CoV-2 in an in vitro setting. NOQ 19 is an unique blend of 13 Ayurvedic herbs.Methodology: Vero E6 (CL1008), the African green monkey kidney epithelial cell, were infected with SARS-CoV-2 virus (isolate USA-WA1/2020) in a 96 well-plate. NOQ19 test material was diluted in different concentration as follows 0.05mg/ml, 0.1mg/ml, 0.2mg/ml, 0.3mg/ml, 0.4mg/ml, 0.5mg/ml, 0.6mg/ml, 0.7mg/ml, 0.8mg/ml and 0.9mg/ml. These different concentrations of NOQ19 were added to infected cells respectively and incubated for 3 days in 5% CO2 incubator. Remdesivir was used as a positive control.The cells were finally fixed with formaldehyde, stained with crystal violet and plaques were visualized. The number of plaques were counted to determine the PFU(plaque forming units)/mL.Results: Results demonstrated 100% antiviral efficacy of NOQ19 at 0.9mg/ml concentration with complete elimination of the virus. The IC50 of the drug was found to be 0.2mg/ml. The results of the present study demonstrated viral load reduction in SARS- CoV-2 infected Vero E6 cell lines.Conclusion: The result along with clinical trials could propose NOQ19 as a potential therapeutic option in the fighting the COVID-19 challenge.

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“…The viral load reduction as measured by PCR was noted as 6.241 × 103 IU/mL at 25mg/ml concentration. [21] A study on Glycyrrhizin, a major component of Yashtimadhu,…”

Section: Discussionmentioning

confidence: 99%

An In-vitro evaluation of a polyherbal formulation, against SARS-CoV-2

Kanchibhotla¹,

Subramanian²,

reddy³

et al. 2021

Preprint

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“…As a result, it might be a promising option for managing SARS-CoV-2 either as a preventive, a primary symptomatic treatment, or a substitute medication (Mofed et al, 2020).…”

Section: Withania Somnifera: Therapeutically Potential Activitiesmentioning

confidence: 99%

Phyto‐therapeutic potential of Withania somnifera: Molecular mechanism and health implications

Yadav,

Tripathi,

Sangwan

2024

Phytotherapy Research

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Withania somnifera, the plant named Indian ginseng, Ashwagandha, or winter cherry, has been used since ancient times to cure various health ailments. Withania somnifera is rich in constituents belonging to chemical classes like alkaloids, saponins, flavonoids, phenolic acids, and withanolides. Several chemotypes were identified based on their phytochemical composition and credited for their multiple bioactivities. Besides, exhibiting neuroprotective, immunomodulatory, adaptogenic, anti‐stress, bone health, plant has shown promising anti‐cancer properties. Several withanolides have been reported to play a crucial role in cancer; they target cancer cells by different mechanisms such as modulating the expression of tumor suppressor genes, apoptosis, telomerase expression, and regulating cell signaling pathway. Though, many treatments are available for cancer; however, to date, no assured reliable cure for cancer is made available. Additionally, synthetic drugs may lead to development of resistance in time; therefore, focus on new and natural drugs for cancer therapeutics may prove a longtime effective alternative. This current report is a comprehensive combined analysis upto 2023 with articles focused on bio‐activities of plant Withania somnifera from various sources, including national and international government sources. This review focuses on understanding of various mechanisms and pathways to inhibit uncontrolled cell growth by W. somnifera bioactives, as reported in literature. This review provides a recent updated status of the W. somnifera on pharmacological properties in general and anti‐cancer in particular and may provide a guiding resource for researchers associated with natural product‐based cancer research and healthcare management.

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“…Several studies have shown the hepatoprotective activity of this plant without toxicity [ 127 , 128 ]. Withaferin A performs a crucial function as antiviral agents against several viruses, including HIV-1 [ 129 ], HPV [ 130 ], HSV [ 131 ], and infectious bursal diseases virus (IBDV) [ 131 ]. In a recent study, it has been reported that withaferin-A and withanone could bind and stably interact with the catalytic site of TMPRSS2, suggesting their use in the blocking of COVID-19 viral entry into host cells [ 132 ].…”

Section: Hepatoprotective Agentsmentioning

confidence: 99%

Role of Nutraceuticals in COVID-19 Mediated Liver Dysfunction

et al. 2020

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COVID-19 is known as one of the deadliest pandemics of the century. The rapid spread of this deadly virus at incredible speed has stunned the planet and poses a challenge to global scientific and medical communities. Patients with COVID-19 are at an increased risk of co-morbidities associated with liver dysfunction and injury. Moreover, hepatotoxicity induced by antiviral therapy is gaining importance and is an area of great concern. Currently, alternatives therapies are being sought to mitigate hepatic damage, and there has been growing interest in the research on bioactive phytochemical agents (nutraceuticals) due to their versatility in health benefits reported in various epidemiological studies. Therefore, this review provides information and summarizes the juncture of antiviral, immunomodulatory, and hepatoprotective nutraceuticals that can be useful during the management of COVID-19.

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The Antiviral Efficacy of <i>Withania somnifera</i> (Ashwagandha) against Hepatitis C Virus Activity: <i>In Vitro</i> and <i>in Silico</i> Study

Cited by 13 publications

References 30 publications

An In-vitro evaluation of a polyherbal formulation, against SARS-CoV-2

An In-vitro evaluation of a polyherbal formulation, against SARS-CoV-2

Phyto‐therapeutic potential of Withania somnifera: Molecular mechanism and health implications

Role of Nutraceuticals in COVID-19 Mediated Liver Dysfunction

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