The antithrombotic and antimicrobial properties of PEG-protected silver nanoparticle coated surfaces

Ragaseema, V.M.; Unnikrishnan, Sneha; Krishnan, V. Kalliyana; Krishnan, Lissy K.

doi:10.1016/j.biomaterials.2012.01.005

Cited by 105 publications

(70 citation statements)

References 18 publications

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“…Usually, the AgNPs are considered cytotoxic. We showed that the AgNPs are not toxic to the cells, confirming recent work by Yen et al 26 and Ragaseema et al, 27 and that they even promote growth. This may be associated with the method we used to produce the NPs.…”

Section: Discussionsupporting

confidence: 91%

“…Although AgNPs were reported to have a cytotoxic effect on cells, [23][24][25] and their use in medical applications is not widespread, recent studies reveal that AgNPs can be biocompatible. 26 Ragaseema et al 27 have demonstrated antibacterial, antiplatelet, and noncytotoxic properties of AgNPs for the use in cardiovascular implantable devices. Fullenkamp et al 28 developed silver (Ag)-releasing hydrogels, which showed no effect on the viability of mammalian cell lines.…”

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confidence: 99%

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Substrates coated with silver nanoparticles as a neuronal regenerative material

Alon

Miroshnikov

Perkas

et al. 2014

IJN

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Much effort has been devoted to the design of effective biomaterials for nerve regeneration. Here, we report the novel use of silver nanoparticles (AgNPs) as regenerative agents to promote neuronal growth. We grew neuroblastoma cells on surfaces coated with AgNPs and studied the effect on the development of the neurites during the initiation and the elongation growth phases. We find that the AgNPs function as favorable anchoring sites, and the growth on the AgNP-coated substrates leads to a significantly enhanced neurite outgrowth. Cells grown on substrates coated with AgNPs have initiated three times more neurites than cells grown on uncoated substrates, and two times more than cells grown on substrates sputtered with a plain homogenous layer of silver. The growth of neurites on AgNPs in the elongation phase was enhanced as well. A comparison with substrates coated with gold nanoparticles (AuNPs) and zinc oxide nanoparticles (ZnONPs) demonstrated a clear silver material-driven promoting effect, in addition to the nanotopography. The growth on substrates coated with AgNPs has led to a significantly higher number of initiating neurites when compared to substrates coated with AuNPs or ZnONPs. All nanoparticle-coated substrates affected and promoted the elongation of neurites, with a significant positive maximal effect for the AgNPs. Our results, combined with the well-known antibacterial effect of AgNPs, suggest the use of AgNPs as an attractive nanomaterial – with dual activity – for neuronal repair studies.

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Section: Discussionsupporting

confidence: 91%

mentioning

confidence: 99%

Substrates coated with silver nanoparticles as a neuronal regenerative material

Alon

Miroshnikov

Perkas

et al. 2014

IJN

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“…4 The nanoparticles showed potent antibacterial activity and a significantly higher synergistic effect with erythromycin, methicillin, and ciprofloxacin. 5 Silver nanoparticles are also reported to possess antifungal, 6 anti-inflammatory, 7 antiviral, 8 anti-angiogenesis, 9 and antiplatelet 10 activities. They all confer to them a major advantage for the development of alternative products against, for example, multidrug-resistant microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Green synthesis of silver nanoparticles by Chrysanthemum morifolium Ramat. extract and their application in clinical ultrasound gel

He¹,

Du²,

Lv³

et al. 2013

IJN

143

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Eco-friendly green synthesis with plant extracts plays a very important role in nanotechnology, without any harmful chemicals. In this report, the synthesis of water-soluble silver nanoparticles was developed by treating silver ions with Chrysanthemum morifolium Ramat. extract at room temperature. The effect of the extract on the formation of silver nanoparticles was characterized by ultraviolet and visible absorption spectroscopy, X-ray diffraction, transmission electron microscopy, and Fourier transform infrared spectroscopy. The ultraviolet and visible absorption spectroscopy results show a strong resonance centered on the surface of silver nanoparticles (AgNP) at 430 nm. The Fourier transform infrared spectroscopy spectral study demonstrates Chrysanthemum morifolium Ramat. extract acted as the reducing and stabilizing agent during the synthesis. The X-ray diffraction analysis confirmed that the synthesized AgNP are single crystallines, corresponding with the result of transmission electron microscopy. Water-soluble AgNP, with an approximate size of 20 nm-50 nm were also observed in the transmission electron microscopy image. The bactericidal properties of the synthesized AgNP were investigated using the agar-dilution method and the growth-inhibition test. The results show the AgNP had potent bactericidal activity on Staphylococcus aureus and Escherichia coli, as well as a strong antibacterial activity against gram-negative bacteria, as compared to gram-positive bacteria with a dose-dependent effect, thus providing a clinical ultrasound gel with bactericidal property for prevention of cross infections.

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“…For this, it is important to find alternative therapies or drugs to overcome these drawbacks. 32 Nanotechnology has contributed in several applications, such as therapeutics, 33 catalysis, 34 biosensing devices, 35 biomedicine due to its ready manufacturing process, 36 quality control, and biocompatibility, and indeed its use in wound healing and antibacterial applications are already part of clinical practice. 22,37 AgNPs have been shown to induce the apoptotic pathway in vitro through free oxygen radical generation, which showed antitumor, antiproliferative, and antiangiogenic effects in vitro.…”

mentioning

confidence: 99%

Green synthesis of silver nanoparticles using Ganoderma neo-japonicum Imazeki: a potential cytotoxic agent against breast cancer cells

Gurunathan¹,

Raman²,

Malek³

et al. 2013

IJN

125

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Background Silver nanoparticles (AgNPs) are an important class of nanomaterial for a wide range of industrial and biomedical applications. AgNPs have been used as antimicrobial and disinfectant agents due their detrimental effect on target cells. The aim of our study was to determine the cytotoxic effects of biologically synthesized AgNPs using hot aqueous extracts of the mycelia of Ganoderma neo-japonicum Imazeki on MDA-MB-231 human breast cancer cells. Methods We developed a green method for the synthesis of water-soluble AgNPs by treating silver ions with hot aqueous extract of the mycelia of G. neo-japonicum. The formation of AgNPs was characterized by ultraviolet-visible absorption spectroscopy, X-ray diffraction, dynamic light scattering, and transmission electron microscopy. Furthermore, the toxicity of synthesized AgNPs was evaluated using a series of assays: such as cell viability, lactate dehydrogenase leakage, reactive oxygen species generation, caspase 3, DNA laddering, and terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labeling in human breast cancer cells (MDA-MB-231). Results The ultraviolet-visible absorption spectroscopy results showed a strong resonance centered on the surface of AgNPs at 420 nm. The X-ray diffraction analysis confirmed that the synthesized AgNPs were single-crystalline, corresponding with the result of transmission electron microscopy. Treatment of MDA-MB-231 breast cancer cells with various concentrations of AgNPs (1–10 μg/mL) for 24 hours revealed that AgNPs could inhibit cell viability and induce membrane leakage in a dose-dependent manner. Cells exposed to AgNPs showed increased reactive oxygen species and hydroxyl radical production. Furthermore, the apoptotic effects of AgNPs were confirmed by activation of caspase 3 and DNA nuclear fragmentation. Conclusion The results indicate that AgNPs possess cytotoxic effects with apoptotic features and suggest that the reactive oxygen species generated by AgNPs have a significant role in apoptosis. The present findings suggest that AgNPs could contribute to the development of a suitable anticancer drug, which may lead to the development of a novel nanomedicine for the treatment of cancers.

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The antithrombotic and antimicrobial properties of PEG-protected silver nanoparticle coated surfaces

Cited by 105 publications

References 18 publications

Substrates coated with silver nanoparticles as a neuronal regenerative material

Substrates coated with silver nanoparticles as a neuronal regenerative material

Green synthesis of silver nanoparticles by Chrysanthemum morifolium Ramat. extract and their application in clinical ultrasound gel

Green synthesis of silver nanoparticles using Ganoderma neo-japonicum Imazeki: a potential cytotoxic agent against breast cancer cells

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