The Antioxidative Property of Nitroglycerin Enhances Lornoxicam’s Antiinflammatory Effect

Abstract

Lornoxicam is a NSAID with a plasma half-life of 3 to 5 hours [9]. Although different NSAIDs have been combined with nitric oxide donors in previous studies, systemic administration of a mixture of lornoxicam and nitroglycerin has not been investigated on nociception to the best of our knowledge. The aim of this study is firstly, to investigate the effect of combination of nitroglycerin and lornoxicam on nociception and secondly, to determine the antioxidative effects of this combination in rats. Material Methods Animals: Male Wistar rats weighing approximately 300-450 g were obtained from Adnan Menderes University Medical Faculty of Animal Ressearch Labarotory Center (Aydin, Turkey). They were housed in polypropylene cages in groups of five per cage, and received standard laboratory chow and tap water ad libitum with 12/12 hours light/dark cycles. All experimental protocols were approved by Animal Ethics Committee of Adnan Menderes University Medical Faculty. Drugs: Lornoxicam (Xefo®, Abdi Ibrahim, Istanbul, Turkey), and nitroglycerin (Perlinganit®, Adeka, Istanbul, Turkey) were used. Drug solutions were prepared so that the desired dose, expressed in terms of saline, was contained in a volume 10 ml kg-1 of body weight for intraperitoneal (i.p.) injection 30 minutes before nociceptive test. Thirty-nine Wistar male rats were divided into five groups; control group (No drug was given to control group, Group C, n=7), isotonic group (0.09 sodium chloride, Group ISO, n=8), lornoxicam group (lornoxicam 1.3 mg kg-1 , Group L, n=8), nitroglycerin group (nitroglycerin 1 mg kg-1 , Group N, n=8), and lornoxicam-nitroglycerin combination group (1.3 mg kg-1 lornoxicam + 1 mg kg-1 nitroglycerin Group L+N, n=8). Assessment of central nociception Hot plate assay: In this test, animals were individually placed on a hot

Comparative study of piroxicam and nitroglycerin on prostaglandin-modulated blood and electrolyte indices during di-estrous in female Wistar Rats

“…In addition to earlier reports linking use of NSAIDs to renal impairment ( Kim, 2008 ; Bennett et al ., 1996 ; Aprioku & Uche, 2013 ), this study also found elevated blood urea levels in piroxicam-treated animals in di-estrous. The positive effect of nitroglycerin on the renal excretion of metabolic waste might be attributed to its anti-oxidative activity ( Sen et al ., 2011 ).…”

“…Group 2 was treated with Piroxicam (3 mg/kg) (Sharp & Villano, 2012) and Group 3 was treated with Nitroglycerin (1 mg/kg) (Sen et al, 2011) All treatments were administered orally for four days.…”

Anesthesia and Intensive Care

“…The aim of the study is to evaluate the effect of combination of nitroglycerin and lornoxicam on nociception and antioxidative system in rats, by taking 39 Wistar male rats as sample. Finally result showed that Nitroglycerin enhances the antioxidative effects of lornoxicam for antinociception [16,33,45] but different mechanisms might also play a role on antinociception. Further studies must be carried out with experimental models and different drug doses to reach an ultimate conclusion.…”

Anesthesia and Intensive Care

“…Finally result showed that Nitroglycerin enhances the antioxidative effects of lornoxicam for antinociception [16,33,45] but different mechanisms might also play a role on antinociception. Further studies must be carried out with experimental models and different drug doses to reach an ultimate conclusion.…”