The Antioxidant Properties of Pectin Fractions Isolated from Vegetables Using a Simulated Gastric Fluid

Abstract

The antioxidant properties of vegetable pectin fractions against intraluminal reactive oxygen species were elucidated in vitro in conjunction with their structural features. The pectin fractions were isolated using a simulated gastric fluid (pH 1.5, pepsin 0.5 g/L, 37 ∘ C, 4 h) from fresh white cabbage, carrot, onion, and sweet pepper. The fraction from onion was found to inhibit the production of superoxide radicals by inhibiting the xanthine oxidase. The high molecular weight of onion pectin and a large number of galactose residues in its side chains appeared to participate in interaction with xanthine oxidase. All the isolated pectic polysaccharides were found to be associated with protein (2-9%) and phenolics (0.5-0.7%) as contaminants; these contaminants were shown to be responsible for the antioxidant effect of vegetable pectin fractions against the hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radicals.

Enzymatically Extracted Apple Pectin Possesses Antioxidant and Antitumor Activity

“…The presence of phenols in pectin of various sources was discussed previously by other researchers [ 22 ]. For example, sugar beet pectin is regarded as particularly rich in phenols, as it contains up to 0.7% (calculated as ferulic acid equivalents), when extracted at high pH [ 23 ], and a ferulation degree (DF) that oscillates around 0.27% [ 24 ].…”

“…Furthermore, phenolic compounds, especially phenolic acids, can play an important role in the overall radical scavenging ability of the same polysaccharides, for example, xylans and xylooligosaccharides [ 29 ]. Smirnov et al [ 22 ] even suggest that a very high DPPH • and • OH scavenging activity of their pectin preparations resulted from a high content of phenolic compounds (more precisely: the compounds that reacted with Folin–Ciocalteu reagent) and protein. Ferulic acid in the effective doses of our pectin preparations (IC 50 , RP 0.5 ) constituted between 0.06 and 0.89 µg.…”

Phyto-Mediated Photo Catalysed Green Synthesis of Silver Nanoparticles Using Durio Zibethinus Seed Extract: Antimicrobial and Cytotoxic Activity and Photocatalytic Applications

“…The selection criteria for durian seed was based on several factors, viz. the presence of saccharides, which are responsible for the reduction of Ag + in reaction solution, amino acids for stabilization of the formed silver nanoparticles, and preference in this area of research, and as it is a waste that causes environmental pollution [24,25,26,27,28].…”

Structural characterization and determination of biological activities for different polysaccharides extracted from tree mushroom species

“…IC 50 values of the polysaccharide extracts and standard compounds (EDTA, α-tocopherol, and BHA) were given in Table 3. Figure 5 shows Triple helix stereo-configuration, higher degrees of branching, moderate molecular weight, and water solubility are other factors that could contribute antioxidant properties (Kungel et al, 2018;Smirnov et al, 2017;Wang et al, 2013;Wang, Hu, Nie, Yu, & Xie, 2015;Xiao & Jiang, 2015;Zhao et al, 2018). The molecular weight of a polysaccharide has a pronounced effect on the biological activity, generally, low molecular weight polysaccharides exhibit higher antioxidant activity than high molecular weight polysaccharides (Sheng & Sun, 2014).…”