The Antioxidant and Cytotoxic Effects of Cosmos caudatus Ethanolic Extract on Cervical Cancer

Abstract

BACKGROUND: Oxidative stress is closely related to all aspects of cancer. Cosmos caudatus ethanolic extract (CCEE) has been proved to have antioxidant effect that inhibited cancer cell growth due to its bioactive compounds such as catechin, quercetin and chlorogenic acid. This study aimed to evaluate antioxidant and anticancer activity of CCEE and its compounds.METHODS: Total phenol was measured according to the Folin-Ciocalteu method. Catechin, quercetin and chlorogenic acid contained in CCEE were identified by high-performance liquid chromatography (HPLC). Antioxidant activity was evaluated by 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS)-reducing activity, 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, and ferric reducing antioxidant power (FRAP) activity test. The cytotoxic activity of CCEE was determined by MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] assay on HeLa cells.RESULTS: The result showed that total phenol of CCEE was 181.64±0.93 µg Cathecin/mg extract. ABTSreducing activity test showed that catechin had the highest activity (2.90±0.04 µg/mL), while CCEE had moderate activity compared to other compounds. FRAP activity test demonstrated that catechin had the highest activity (315.83 µM Fe(II)/µg) compared to other compounds. DPPH scavenging activity of CCEE was 22.82±0.05 µg/mL. Cytotoxicity test on HeLa cell showed that CCEE had lower activity (inhibitory concentration (IC)50= 89.90±1.30 µg/mL) compared to quercetin (IC50 = 13.30±0.64 µg/ mL).CONCLUSION: CCEE has the lowest antioxidant activity compared to quercetin, catechin, and chlorogenic acid and has the lowest anticancer activity compared to quercetin. However, CCEE and its compounds has potential as antioxidant and anticancer properties.KEYWORDS: antioxidant, anticancer, catechin, Cosmos caudatus, quercetin

Antioxidant, Anti-Collagenase, Anti-Elastase and Anti-Tyrosinase Activities of an Aqueous Cosmos caudatus Kunth (Asteraceae) Leaf Extract

“…The difference in CCW activities between the two assays may be caused by the structure difference of the preformed radicals. 20 C. caudatus leaf extracts have been shown to have high antioxidant activities in different studies [21][22][23][24][25] . In one study, the antioxidant capacity of C. caudatus was reported to have the highest potency among 21 tropical plant extracts evaluated, and was comparable in potency to some synthetic antioxidants.…”

“…Previous studies have revealed that the antioxidant activities associated with this species may be attributed to the flavonoids present, while vitamin C may also contribute to the observed antioxidant effects. 21 Flavonoids of the flavonol and flavone subclasses are widely documented to mediate antioxidant effects. [27][28][29] In this study, high-resolution LC-UV-MS/MS analysis of CCW revealed the detection of compounds assigned as quercetin, kaempferol and apigenin glycosides assigned from their observed [M + H] + ions (Table 3) [27][28][29][30] it may be concluded that their detection in CCW may have contributed considerably to the antioxidant effects observed.…”

AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN KENIKIR (Cosmos caudatus Kunth) DENGAN BERBAGAI METODE EKSTRAKSI (ANTIOXIDANT ACTIVITY OF LEAVES EXTRACT OF KENIKIR (Cosmos caudatus Kunth) WITH VARIOUS EXTRACTION METHODS)

“…[6][7] [8] Aktivitas biologik lainnya meliputi antidiabetes, antihiperlipidemia dan sitotoksik. [9][10] [11] . Beberapa penelitian juga telah mengungkapkan mengenai keterkaitan kandungan senyawa metabolit sekunder dan aktivitasnya.…”

The ethanol extract of the bastard cedar (Guazuma ulmifolia L.) as antioxidants

“…Total phenolic content was asserted as gallic acid equivalence (GAE) in μg/mg sample. This test was done triplicate (Nurhayati et al, 2018;Rusmana et al, 2017;Widowati et al, 2015;Prahastuti et al, 2019b).…”