The antioxidant activity of Chinese herbs for eczema and of placebo herbs — I

Kirby, Amanda; Schmidt, Richard J.

doi:10.1016/s0378-8741(97)01510-9

Cited by 326 publications

(170 citation statements)

References 30 publications

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“…[15,16] Briefly, a 50 µl volume of various dilutions of each samples was mixed with 5 ml of 0.004% methanol solutions of DPPH followed by 30 min incubation at ambient temperature. Thereafter, absorbance values of the sample were recorded against control at 517 nm.…”

Section: Antioxidant Activitymentioning

confidence: 99%

Phytochemistry, Antioxidant, and Lipid Peroxidation Inhibition of the Essential Oils ofLavandula OfficinalisL. in Iran

Miri

2018

International Journal of Food Properties

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The aim of present study was to evaluation the antioxidant potential of Lavandula officinalis on the basis of the chemical compositions of oils obtained by hydrodistillation. In the case of L.officinalis, thirteen compounds were identified representing the 96.53% of the total oil. The major constituents of the oil were described as α-pinene (20.14%), camphor (14.36%), menthol (32.51%) and 1,8-cineole (20.14%). The oils were also subjected to screening for their possible antioxidant activity by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays. %). 1,8-Cineole and menthol showed appreciable antioxidant activity in DPPH test. Antioxidant activity guided fractionation of the oil was carried out by The TLC-bioautography screening and fractionation resulted in the separation of the main antioxidant compound which were identified as 1,8-cineole (48%) and menthol (39%).

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Section: Antioxidant Activitymentioning

confidence: 99%

Phytochemistry, Antioxidant, and Lipid Peroxidation Inhibition of the Essential Oils ofLavandula OfficinalisL. in Iran

Miri

2018

International Journal of Food Properties

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“…Active compounds were detected as yellow spots on a white background zones, where the colors changed within 30-60 min (after spraying) were taken as positive results. The other developed TLC sheet was sprayed with a 0.2% solution of the stable radical diphenylpicrylhydrazyl (DPPH) (Cuendet et al, 1997;Kirby & Schmidt, 1997). In these plates, active compounds were detected as yellow spots on a purple background.…”

Section: Rapid Tlc Screening For Antioxidantmentioning

confidence: 99%

“…In this spectrophotometric method, DPPH was used as a reagent in order to measure DPPH free radical scavenging activity (Cuendet et al, 1997;Kirby & Schmidt, 1997). Various concentrations (50 mL) of the volatile oils and some of the main components of the essential oils as well as butylhydroxytoluene (BHT) and vitamin E were added to 2.5 mL of an 0.004% solution of DPPH in methanol.…”

Section: Dpph Free Radical Scavenging Activitymentioning

confidence: 99%

Antioxidant Activity of the Essential Oils of Different Parts ofJuniperus communis. subsp.hemisphaerica. andJuniperus oblonga.

Emami

Javadi

Hassanzadeh

2007

Pharmaceutical Biology

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The essential oils of different parts of Juniperus communis subsp. hemisphaerica (Presl) Nyman (Cupressaceae) and Juniperus oblonga M. B. were examined for their potential radical scavenging activity. The compositions of the essential oils of these plants were studied qualitatively and quantitatively by GC and GC-MS. The main components of the essential oils as well as positive controls were subjected to antioxidant testing. A rapid evaluation for antioxidants, using two TLC screening methods, showed that all tested oils and their main components have antioxidant activity. The abilities of the volatile oils to act as nonspecific donors for hydrogen atoms for electron were checked in the diphenylpicrylhydrazyl (DPPH) assay. In the DPPH assay, the strongest effect among the essential oils was measured for the oil of leaves of male J. communis subsp. hemisphaerica at a concentration of 4 mL=mL (24.0%) In the deoxyribose degradation assay, the essential oils, pure components, and positive controls were tested at different concentrations. Most of the tested compounds showed some antioxidant effects. The fruit oil of J. oblonga has the strongest effect among the tested volatile oils.The deoxyribose assay was modified in three different ways to assess whether the oils exhibited site-specific effects. The results of the current study, which demonstrate the DPPH scavenging activity of the essential oils of the of leaves of male J. communis subsp. hemisphaerica and the OH radical scavenging effects of the fruit oil of J. oblong suggest the use of these two essential oils in very low concentrations for preserving food materials.

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“…Inoscavin E exhibited potent ABTS radical cation scavenging activity with an IC 50 value of 22 m M, which was higher activity than trolox (IC 50 , 30 mM), a well-known antioxidant used as control, and less activity than caffeic acid (IC 50 , 10 mM). Superoxide radical anion scavenging activity was evaluated by the xanthine/xanthine oxidase method with minor modifications [7]. Although it was three-times less active than caffeic acid, a well-known superoxide radical scavenger, inoscavin E displayed significant superoxide radical anion scavenging activity with an IC 50 value of 49 m M. However, inoscavin E showed no DPPH radical scavenging activity up to 100 mM.…”

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confidence: 99%

Inoscavin E, a Free Radical Scavenger from the Fruiting Bodies of Inonotus xeranticus

et al. 2007

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A new free radical scavenger named inoscavin E was isolated from the fruiting bodies of Inonotus xeranticus. Its structure was determined as 2-(3,4-dihydroxyphenyl)-6-[(1E)-2-(3,4-dihydroxyphenyl)ethenyl]-4H-furo[3,2-c]pyran-4-one on the basis of spectroscopic analysis. Inoscavin E exhibited significant scavenging activity against the superoxide radical anion and ABTS radical cation. Keywords inoscavin E, Inonotus xeranticus, free radical scavengerInonotus xeranticus (Berk.) Imaz. Et Aoshi. (Hymenochaetaceae) is a saprophytic fungus preferably living on deciduous trees and widely distributes in East Asia [1]. In previous investigation on antioxidant constituents from this mushroom, we isolated several free radical scavengers, inoscavins AϳD [2] and interfungins AϳC [3]. Our continuing search for new antioxidant principles from this material, a new free radical scavenger, designated as inoscavin E, has been isolated. In this paper, the isolation, structure determination, and free radical scavenging activity of inoscavin E are described.The fresh fruiting bodies of Inonotus xeranticus were crushed and extracted with methanol at room temperature. The methanolic extract was concentrated under reduced pressure and the aqueous resultant was partitioned with hexane. The hexane layer was discarded, and EtOAc was added to the water phase and shaken. The EtOAc layer was concentrated and subjected to a column of Sephadex LH-20 eluting with a mixture of CHCl 3 : MeOH (1 : 1, v/v). The antioxidant fraction was rechromatographed on a column of Sephadex LH-20 eluting with MeOH only, followed by reversed-phase column chromatography eluting with 70% aqueous MeOH. Finally, 4.0 mg of inoscavin E was purified by preparative reversed-phase TLC developed with 80% aqueous MeOH.The molecular weight of inoscavin E was determined to

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The antioxidant activity of Chinese herbs for eczema and of placebo herbs — I

Cited by 326 publications

References 30 publications

Phytochemistry, Antioxidant, and Lipid Peroxidation Inhibition of the Essential Oils ofLavandula OfficinalisL. in Iran

Phytochemistry, Antioxidant, and Lipid Peroxidation Inhibition of the Essential Oils ofLavandula OfficinalisL. in Iran

Antioxidant Activity of the Essential Oils of Different Parts ofJuniperus communis. subsp.hemisphaerica. andJuniperus oblonga.

Inoscavin E, a Free Radical Scavenger from the Fruiting Bodies of Inonotus xeranticus

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