The antimicrobial efficacy of sustained release silver–carbene complex-loaded l-tyrosine polyphosphate nanoparticles: Characterization, in vitro and in vivo studies

Hindi, Khadijah M.; Ditto, Andrew J.; Panzner, Matthew J.; Medvetz, Douglas A.; Han, Daniel; Hovis, Christine E.; Hilliard, Julia K.; Taylor, Jane B.; Yun, Yang; Cannon, Carolyn L.; Youngs, Wiley J.

doi:10.1016/j.biomaterials.2009.03.044

Cited by 161 publications

(122 citation statements)

References 42 publications

(51 reference statements)

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“…A silver carbene complex loaded on L-tyrosine polyphosphate (SCC) NPs and then administered to Pseudomonas aeruginosa-infected mice resulted in a 20% survival advantage. Further, in the same study, the lungs from the majority of the mice treated with SCC NPs appeared normal, with a significant decrease in the bacterial burdens in the lungs (71).…”

Section: Differential Efficacy Of Silver Nanoparticlesmentioning

confidence: 65%

Interaction of Silver Nanoparticles with Serum Proteins Affects Their Antimicrobial Activity In Vivo

Gnanadhas

Thomas

et al. 2013

Antimicrob Agents Chemother

144

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dThe emergence of multidrug-resistant bacteria is a global threat for human society. There exist recorded data that silver was used as an antimicrobial agent by the ancient Greeks and Romans during the 8th century. Silver nanoparticles (AgNPs) are of potential interest because of their effective antibacterial and antiviral activities, with minimal cytotoxic effects on the cells. However, very few reports have shown the usage of AgNPs for antibacterial therapy in vivo. In this study, we deciphered the importance of the chosen methods for synthesis and capping of AgNPs for their improved activity in vivo. The interaction of AgNPs with serum albumin has a significant effect on their antibacterial activity. It was observed that uncapped AgNPs exhibited no antibacterial activity in the presence of serum proteins, due to the interaction with bovine serum albumin (BSA), which was confirmed by UV-Vis spectroscopy. However, capped AgNPs [with citrate or poly(vinylpyrrolidone)] exhibited antibacterial properties due to minimized interactions with serum proteins. The damage in the bacterial membrane was assessed by flow cytometry, which also showed that only capped AgNPs exhibited antibacterial properties, even in the presence of BSA. In order to understand the in vivo relevance of the antibacterial activities of different AgNPs, a murine salmonellosis model was used. It was conclusively proved that AgNPs capped with citrate or PVP exhibited significant antibacterial activities in vivo against Salmonella infection compared to uncapped AgNPs. These results clearly demonstrate the importance of capping agents and the synthesis method for AgNPs in their use as antimicrobial agents for therapeutic purposes.

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Section: Differential Efficacy Of Silver Nanoparticlesmentioning

confidence: 65%

Interaction of Silver Nanoparticles with Serum Proteins Affects Their Antimicrobial Activity In Vivo

Gnanadhas

Thomas

et al. 2013

Antimicrob Agents Chemother

144

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show abstract

“…This difference in airway inflammation was reflected in the BAL fluid from water-and SCC1-treated animals; the total leukocyte and neutrophil counts recovered from the SCC1-treated animals were significantly reduced ( Fig. 8B and C (16,17,28,29,31,33). In particular, the MIC of SCC1 for the mucoid clinical strain of P. aeruginosa studied here, strain PA M57-15, is 2 g/ml.…”

Section: Vol 53 2009 Nebulized Scc1 For Pulmonary Infections 3289mentioning

confidence: 80%

“…This report is the first to investigate the direct nebulization of a silver carbene complex in solution as a potential therapy for pulmonary infections. Given that SCC1 appears to be safe and effective in these animal models of lung infection, further studies of SCC1 and other silver carbene compounds, either directly aerosolized in solution or in a nanoparticle suspension (17), appear to be warranted. Nebulized silver-based antimicrobials may serve as useful adjuncts to the current therapies for difficult-to-treat pulmonary infections.…”

Section: Vol 53 2009 Nebulized Scc1 For Pulmonary Infections 3289mentioning

confidence: 99%

In Vitro and Murine Efficacy and Toxicity Studies of Nebulized SCC1, a Methylated Caffeine-Silver(I) Complex, for Treatment of Pulmonary Infections

Cannon¹,

Hogue²,

Vajravelu³

et al. 2009

Antimicrob Agents Chemother

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The expanding clinical challenge of respiratory tract infections due to resistant bacteria necessitates the development of new forms of therapy. The development of a compound composed of silver coupled to a methylated caffeine carrier (silver carbene complex 1 [SCC1]) that demonstrated in vitro efficacy against bacteria, including drug-resistant organisms, isolated from patients with respiratory tract infections was described previously. The findings of current in vitro studies now suggest that bactericidal concentrations of SCC1 are not toxic to airway epithelial cells in primary culture. Thus, it was hypothesized that SCC1 could be administered by the aerosolized route to concentrate delivery to the lung while minimizing systemic toxicity. In vivo, aerosolized SCC1 delivered to mice resulted in mild aversion behavior, but it was otherwise well tolerated and did not cause lung inflammation following administration over a 5-day period. The therapeutic efficacy of SCC1 compared to that of water was shown in a 3-day prophylaxis protocol, in which mice infected with a clinical strain of Pseudomonas aeruginosa had increased survival, decreased amounts of bacteria in the lung, and a lower prevalence of bacteremia. Similarly, by using an airway infection model in which bacteria were impacted in the airways by agarose beads, the administration of SCC1 was significantly superior to water in decreasing the lung bacterial burden and the levels of bacteremia and markers of airway inflammation. These observations indicate that aerosolized SCC1, a novel antimicrobial agent, warrants further study as a potential therapy for bacterial respiratory tract infections.

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“…Baskaran et al Ag-NPs has been well documented in previous studies, 52,78,79 likely because larger specific surface area yields improved antimicrobial activity. 80 The mode of antimicrobial action by Ag-NPs may be through the inhibition of microbial processes on the cell surface and within the cell.…”

mentioning

confidence: 93%

Biosynthesis, characterization, and evaluation of bioactivities of leaf extract-mediated biocompatible silver nanoparticles from an early tracheophyte,<em> Pteris tripartita</em> Sw.

Baskaran

Vigila

Thangaraj

et al. 2016

IJN

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The antimicrobial efficacy of sustained release silver–carbene complex-loaded l-tyrosine polyphosphate nanoparticles: Characterization, in vitro and in vivo studies

Cited by 161 publications

References 42 publications

Interaction of Silver Nanoparticles with Serum Proteins Affects Their Antimicrobial Activity In Vivo

Interaction of Silver Nanoparticles with Serum Proteins Affects Their Antimicrobial Activity In Vivo

In Vitro and Murine Efficacy and Toxicity Studies of Nebulized SCC1, a Methylated Caffeine-Silver(I) Complex, for Treatment of Pulmonary Infections

Biosynthesis, characterization, and evaluation of bioactivities of leaf extract-mediated biocompatible silver nanoparticles from an early tracheophyte,<em> Pteris tripartita</em> Sw.

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