The antimicrobial effect of thyme essential oil, nisin, and their combination against Listeria monocytogenes in minced beef during refrigerated storage

Σολωμάκος, Νικόλαος; Govaris, Α.; Koidis, P.; Botsoglou, Nikolaos

doi:10.1016/j.fm.2007.07.002

Cited by 243 publications

(175 citation statements)

References 31 publications

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“…Thymol is generally recognized as a safe food and many publications have reported thymol had better activity than carvacrol (Bouddine et al 2012). Solomakosa et al (2008) showed that the combined addition of thyme EOs at 0.6 % and nisin at 500 or 1000 IU/g showed a synergistic activity against L. monocytogenes. The authors suggested that such combination could represent a promising approach to control Listeria growth.…”

Section: Discussionmentioning

confidence: 99%

“…Yamazaki et al (2004) reported that the combined effects of nisin, essential oil constituents and diglycerol fatty acid ester could reduce the viable counts of L. monocytogenes. In laboratory study, nisin and thyme EOs combination decrease the population of L. monocytogenes in minced beef during refrigerated storage (Solomakosa et al 2008).…”

Section: Introductionmentioning

confidence: 94%

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Enhanced bactericidal effect of enterocin A in combination with thyme essential oils against L. monocytogenes and E. coli O157:H7

Ghrairi

Hani

2013

J Food Sci Technol

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The combined effects of enterocin A with Thymus vulgaris essential oils (EOs) against Listeria monocytogenes and Escherichia coli O157:H7 were investigated in vitro by enumeration of surviving populations of testing pathogens and minimal inhibitory concentration (MIC) determination. Enterocin A was purified to homogeneity by RP-HPLC from the culture fluid of Enterococcus strain and thyme EOs were extracted from local Thymus vulgaris plants. The major constituent of thyme EOs oils determined by GC-MS was thymol (78.4 %). Combination of enterocin A with thyme EOs showed an enhanced bactericidal effect against Listeria monocytogenes. Checkerboard assay and isobologram construction displayed a synergistic interaction between these compounds against Listeria (FIC index <0.5). Moreover, the MIC value of enterocin A has fallen fivefold (from 4.57 to 0.9 μg/ml), while the MIC of thyme EOs decreased threefold (from 3.6 to 1.2 μg/ml). Treatments with enterocin A alone did not affect the growth of the enteric pathogen E. coli O157:H7. However, the addition of thyme EOs and enterocin A yielded a synergistic antimicrobial effect against E. coli (MIC thyme EOs decrease from 2.2 to 0.71 μg/ml). This is the first report on the combined effect of enterocin A and thyme EOs against food pathogen bacteria. This combination could be useful in food bio-preservation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

Enhanced bactericidal effect of enterocin A in combination with thyme essential oils against L. monocytogenes and E. coli O157:H7

Ghrairi

Hani

2013

J Food Sci Technol

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“…heart diseases, stroke, arteriosclerosis and cancer, as well as being involved in the aging process (Willcox et al, 2004). The effects of plant extracts or essential oils classified as greatly recognized as safe (GRAS) following their addition, have been studied extensively and reported in a variety of meat types, including pork (Nissen et al, 2004); beef (Solomakos et al, 2008); lamb (Camo et al, 2008). Lamb meat contains higher levels of v -3 polyunsaturated fatty acids (PuFAs) compared to beef or pork, which is beneficial to human health (Wood et al, 1999); however, PuFAs increase the susceptibility of meat to oxidative processes such as lipid oxidation ultimately leading to off odors and warmed over flavor (Jeremiah, 2001).…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and antimicrobial effect of some natural plant extracts added to lamb patties during storage

2011

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“…Essa alteração leva à perda de íons com perda do potencial de membrana, colapso da bomba de prótons e depleção de ATP, coagulação citoplasmática e clivagem de macromoléculas (Solomakos et al, 2008). Estudos mostram que os compostos fenólicos dos óleos essenciais se ligam à bicamada fosfolipídica da membrana celular aumentando sua permeabilidade e promovendo o extravasando dos constituintes intracelulares ou danificando o sistema enzimático da célula (Moreira et al, 2005).…”

Section: Resultsunclassified

Adaptação e adaptação cruzada de Listeria monocytogenes aos compostos eugenol e carvacrol

Souza

Tebaldi

Píccoli

2015

Rev. bras. plantas med.

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RESUMO:A evolução de certos microrganismos permite sua rápida adaptação aos ambientes em constante mudança, desenvolvendo assim, tolerância ou resistência ao aumento de determinados estresses. O uso de compostos bioativos provenientes da flora nativa tem sido apontado como uma possível solução para os problemas de controle da resistência e proliferação bacteriana. Este trabalho visou verificar a adaptação e adaptação cruzada de L. monocytogenes, frente aos compostos fenólicos eugenol e carvacrol. A concentração mínima inibitória (CMI) dos compostos fenólicos foi determinada pela técnica de microdiluição em placas de 96 cavidades, em caldo TSB + 0,5% de Tween 80. As concentrações finais (%) obtidas foram: 0,06; 0,12; 0,24; 0,49; 0,98; 1,95; 3,9; 6,25; 12,5; 25; 50. A suspensão bacteriana padronizada foi inoculada nas cavidades das placas, as quais foram incubadas a 37ºC por 24 horas com posterior leitura da absorbância a 620 nm e determinação da CMI. A adaptação das células de L. monocytogenes ao eugenol e carvacrol foi realizada com o cultivo das células em TSB + 0,06% de eugenol ou carvacrol à 37°C por 2 horas. A cultura foi então centrifugada e as células ressuspendidas e padronizadas em TSB. A seguir, realizou-se novamente a técnica de microdiluição em caldo. Os resultados obtidos demonstraram que L. monocytogenes apresentou adaptação e adaptação cruzada frente ao carvacrol e eugenol. A CMI do eugenol e carvacrol foi de 24%. A pré-exposição de L. monocutogenes a concentração sub-letal de 0,06% de carvacrol ou de eugenol aumentou sua resistência. A pré-exposição ao carvacrol promoveu a adaptação de L.monocytogenes a ele aumentando a CMI para 12,5%. Já para o eugenol a CMI passou para 25%. Quando submetidas à concentração sub-letal de eugenol, este promoveu a adaptação das células tanto ao carvacrol quanto ao eugenol, sendo a CMI de 12,5%. Os resultados obtidos demonstraram que L. monocytogenes apresentou adaptação e adaptação cruzada ao carvacrol e eugenol. O presente trabalho sugere estudos futuros ainda mais abrangentes quanto à potencialidade antimicrobiana destes compostos. Palavras-chave: antimicrobianos naturais, compostos bioativos, patógeno alimentarABSTRACT: Adaptation and Cross adaptation of Listeria monocytogenes to eugenol and carvacrol compounds. Some microorganisms have evolved and therefore are able to rapidly adapt themselves to a constantly changing environment, thus developing tolerance or resistance to the increase of some specific stresses. The use of bioactive compounds from the native vegetation has been pointed out as a possible solution to the problems of control of bacterial resistance and proliferation. This work aimed to check the adaptation and cross adaptation of L. monocytogenes, towards the phenolic compounds eugenol and carvacrol. The minimum inhibitory concentration (MIC) of the phenolic compounds was determined by the microdilution technic in plates of 96 cavities, in CALDO TSB + 0,5% of Tween 80. The final concentrations (%) obtained were: 0,06; 0,12; 0,24; 0,49; 0,98; 1,95;...

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The antimicrobial effect of thyme essential oil, nisin, and their combination against Listeria monocytogenes in minced beef during refrigerated storage

Cited by 243 publications

References 31 publications

Enhanced bactericidal effect of enterocin A in combination with thyme essential oils against L. monocytogenes and E. coli O157:H7

Enhanced bactericidal effect of enterocin A in combination with thyme essential oils against L. monocytogenes and E. coli O157:H7

Antioxidant and antimicrobial effect of some natural plant extracts added to lamb patties during storage

Adaptação e adaptação cruzada de Listeria monocytogenes aos compostos eugenol e carvacrol

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