The antimicrobial activity of photodynamic therapy against Streptococci species in dental biofilm using different photosensitizers: An in vitro study

Spinei, Aurelia; Spinei, Iurie

doi:10.1109/ehb.2013.6707409

Cited by 6 publications

(7 citation statements)

References 10 publications

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“…The systematic review showed that among the cariogenic microorganisms listed in the selected studies, the most studied microorganism was Streptococcus mutans (82%) [12,14,15,16,17,18,19,20,21,23,24,25,26,27,28,29,31,32,33,34,35,37,39,40,41,42,43,45].…”

Section: Resultsmentioning

confidence: 99%

“…The success of PDT depends on factors such as the administrated dose of light in the target cells and the time of exposure to light [46]. Considering these factors, we found that the most widely used light source was the red LED (32%) with wavelengths ranging from 625 to 670 nm [12,15,16,17,18,21,24,29,30,32,36,40], the most commonly used PS was methylene blue [13,16,18,21,23,24,27,28,29,30,32,33,36,38,39,42], and the most widely used pre-irradiation time was 5 min [12,14,15,16,17,19,20,21,22,27,28,30,31,32,33,39,42,43,45].…”

Section: Resultsmentioning

confidence: 99%

“…For a substance to be considered as a bactericide, it is necessary for a total inhibition of microorganism growth or ≤99.9% decrease in the initial inoculum (3-log 10 reduction in colony forming units [cfu]/mL) in the subculture [68]. From the 34 articles analyzed, only 11 presented a reduction greater than or equal to 99.9% [14,16,18,23,24,29,34,39,41,43,45]. This fact proves that eliminating these microorganisms is a great challenge, especially when they are in the biofilm.…”

Section: Discussionmentioning

confidence: 99%

“…Among the vitro studies, the biofilm models were more frequent [12,13,15,18,19,20,21,24,27,28,31,32,33,34,35,37,38,39,40,43,44,45] than the suspensions, which confirm the fidelity of the data, since in biofilms, the microorganisms interact with each other and are more resistant to the antimicrobial agents when compared to the microorganisms in suspension [75]. Many studies have used monotypic biofilms of S. mutans , but this model is less representative of the oral environment and underestimates the complexity of the dental biofilm [76], so we emphasize the importance of studies with multispecies biofilms.…”

Section: Discussionmentioning

confidence: 99%

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Effect of Photodynamic Therapy on Microorganisms Responsible for Dental Caries: A Systematic Review and Meta-Analysis

Oliveira

Ferrisse

Marques

et al. 2019

IJMS

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The aim of this study was to perform a systematic review of the literature followed by a meta-analysis about the efficacy of photodynamic therapy (PDT) on the microorganisms responsible for dental caries. The research question and the keywords were constructed according to the PICO strategy. The article search was done in Embase, Lilacs, Scielo, Medline, Scopus, Cochrane Library, Web of Science, Science Direct, and Pubmed databases. Randomized clinical trials and in vitro studies were selected in the review. The study was conducted according the PRISMA guideline for systematic review. A total of 34 articles were included in the qualitative analysis and four articles were divided into two subgroups to perform the meta-analysis. Few studies have achieved an effective microbial reduction in microorganisms associated with the pathogenesis of dental caries. The results highlight that there is no consensus about the study protocols for PDT against cariogenic microorganisms, although the results showed the PDT could be a good alternative for the treatment of dental caries.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Photodynamic Therapy on Microorganisms Responsible for Dental Caries: A Systematic Review and Meta-Analysis

Oliveira

Ferrisse

Marques

et al. 2019

IJMS

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“…The photodynamic approach has emerged as an innovative alternative to antimicrobial regimens and mechanical means for eradicating biofilms, and their application to biofilms has shown superior results compared to the conventional approaches (selected recent PDT studies on biofilms are listed in Table ). It is reported that PDT against Streptococci species in a dental biofilm ensures total destruction of the microorganisms (Spinei ). One‐time photomechanical wave treatment (laser light at 666 nm) in a biofilm of Actinomyces viscosus in the oral cavity increased penetration of methylene blue by up to 75% (Donnelly and others ).…”

Section: Photodynamic Therapymentioning

confidence: 99%

Current and Recent Advanced Strategies for Combating Biofilms

Sadekuzzaman

Yang

Mizan

et al. 2015

Comp Rev Food Sci Food Safe

225

148

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Biofilms are matrix-enclosed microbial aggregates that adhere to a biological or nonbiological surface. Biofilm formation is a significant problem in the medical, food, and marine industries and can lead to substantial economic and health problems. The complex microbial community of a biofilm is highly resistant to antibiotics and sanitizers and confers persistent survival that is a challenge to overcome. There are several conventional approaches to combating biofilms, physical and/or mechanical removal, chemical removal, and the use of antimicrobials, sanitizers, or disinfectants to kill biofilm organisms. However, biofilms are highly resistant to these approaches as opposed to planktonic cells. Thus, novel approaches other than the conventional methods are urgently needed. In this review, we discuss current and new advanced antibiofilm strategies that are superior to the conventional method in terms of addressing the biofilm problem for the improvement of healthcare, food safety, and in industrial processes.

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The effect of photodynamic therapy in controlling the oral biofilm: A comprehensive overview

Aghili,

Jahangirnia,

Alam

et al. 2023

J Basic Microbiol

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Several resistance mechanisms are involved in dental caries, including oral biofilms. An accumulation of bacteria on the surface of teeth is called plaque. Periodontitis and gingivitis are caused by dental plaque. In this review article, we aimed to review the studies associated with the application of photodynamic therapy (PDT) to prevent and treat various microbial biofilm‐caused oral diseases in recent decades. There are several studies published in PubMed that have described antimicrobial photodynamic therapy (APDT) effects on microorganisms. Several in vitro and in vivo studies have demonstrated the potential of APDT for treating endodontic, periodontal, and mucosal infections caused by bacteria as biofilms. Reactive oxygen species (ROS) are activated in the presence of oxygen by integrating a nontoxic photosensitizer (PS) with appropriate wavelength visible light. By causing irreversible damage to microorganisms, ROS induces some biological and photochemical events. Testing several wavelengths has been conducted to identify potential PS for APDT. A standard protocol is not yet available, and the current review summarizes findings from dental studies on APDT.

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The antimicrobial activity of photodynamic therapy against Streptococci species in dental biofilm using different photosensitizers: An in vitro study

Cited by 6 publications

References 10 publications

Effect of Photodynamic Therapy on Microorganisms Responsible for Dental Caries: A Systematic Review and Meta-Analysis

Effect of Photodynamic Therapy on Microorganisms Responsible for Dental Caries: A Systematic Review and Meta-Analysis

Current and Recent Advanced Strategies for Combating Biofilms

The effect of photodynamic therapy in controlling the oral biofilm: A comprehensive overview

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