The Antigenic Heterogeneity of Type 30 Echoviruses12

Wenner, Herbert A.; Harmon, Patricia; Behbehani, Abbas M.; Rouhandeh, H.; Kamitsuka, Paul

doi:10.1093/oxfordjournals.aje.a120687

Cited by 19 publications

(19 citation statements)

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“…Thus, the Giles and PR17 reference strains were included in genotype I, and the Bastianni and Frater prototype strains were included in genotype II. This segregation was supported by the phylogenetic trees obtained from amino acid sequences (data not shown), and it correlated with the antigenic heterogeneity of these reference strains as described in earlier studies (17).…”

Section: Discussionsupporting

confidence: 84%

“…Additionally, this serotype is one of several enteroviruses associated with sporadic cases of aseptic meningitis (5). EV30 is antigenically heterogeneous, and three major antigenic groups have been defined (6,17), although the basis for the antigenic differences is unknown. Previous studies of the molecular epidemiology of EV30 established four genotypes (designated 1 to 4) on the basis of VP1 sequences of 136 geographically dispersed EV30 strains isolated in 10 countries between 1956 and 1998 (10).…”

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confidence: 99%

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Molecular Epidemiology of Echovirus 30: Temporal Circulation and Prevalence of Single Lineages

Palacios

Casas

Cisterna

et al. 2002

J Virol

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Echovirus 30 (EV30) is one of the most frequently isolated EVs, causing extensive outbreaks of EV30 aseptic meningitis in temperate climates. EV30 is antigenically heterogeneous, and three major antigenic groups have been defined, although the basis for the antigenic differences is unknown. A reverse transcription-nested PCR which amplifies the 3-terminal region of the VP1 gene directly from clinical samples was selected for studying EV30 molecular epidemiology, since the major antigenic sites in this region reflect the serotypic pattern of this virus. The different previous approaches to the genetic classification of EV30 were analyzed. A complete study of the EV30 strains was performed by analyzing the sequences from the 112 EV30 strains amplified in this work and the complete set of EV30 strains previously published. A total of 318 strains of EV30 were divided into two broad genotypes (I and II). This classification was supported by the phylogenetic trees obtained from amino acid sequences, and it correlated with the antigenic heterogeneity of the reference strains described in earlier studies. The genotypes could be further divided into subgroups, and these subgroups could be divided into lineages based on their nucleotide distances and levels of bootstrapping. On the other hand, the subgroups and lineages did not result in the same correlation between amino acid and nucleotide differentiation. The molecular epidemiology of EV30 can be compared to influenza virus epidemiology, where prevailing lineages displace the less established lineages on the basis of immune escape. This pattern of evolution is clearly different from that of other enteroviruses. A single lineage at a time appears to be circulating worldwide. This behavior may be related to the epidemic activity of EV30.Human enteroviruses (HEVs) are small RNA viruses of the Picornaviridae family. Genetic and phylogenetic analysis of the capsid-encoding region VP1 showed four clusters: (i) HEV-A, including 11 coxsackie A viruses (CAVs) and enterovirus 71; (ii) HEV-B, including all coxsackie B viruses (CBVs), all echoviruses (EVs), enterovirus 69, and CAV9; (iii) HEV-C, including all polioviruses and 11 other CAVs; and (iv) HEV-D, including enteroviruses 68 and 70. The common transmission routes may be direct, by fecal-oral and respiratory spread, and indirect, by fomites and contaminated water. Enterovirus infections generally go unnoticed, complicating their differential diagnosis. Infection occasionally leads to severe disorders such as meningitis, encephalitis, pleurodynia, myocarditis, conjunctivitis, or severe systemic infections in neonates (7).Echovirus 30 (EV30) is one of the most frequently isolated EVs, causing extensive outbreaks of EV30 aseptic meningitis in temperate climates in several countries (10, 16). Additionally, this serotype is one of several enteroviruses associated with sporadic cases of aseptic meningitis (5). EV30 is antigenically heterogeneous, and three major antigenic groups have been defined (6, 17), although the basis for t...

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Section: Discussionsupporting

confidence: 84%

mentioning

confidence: 99%

Molecular Epidemiology of Echovirus 30: Temporal Circulation and Prevalence of Single Lineages

Palacios

Casas

Cisterna

et al. 2002

J Virol

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“…Antigenic changes among HEV serotypes can cause difficulties in identification by traditional Nt test (37,38).…”

Section: Discussionmentioning

confidence: 99%

Aseptic meningitis by echovirus 30 in São Paulo state, Brazil

Machado¹,

Ferreira²,

Carmona³

et al. 2007

Braz. J. Microbiol.

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The objective of this study was to identify and characterize the immunological and molecular aspects of Human Enterovirus (HEV) associated with aseptic meningitis cases in São Paulo State, Brazil. Clinical samples (cerebrospinal fluid CSF, stool and sera) were analyzed from 21 viral meningitis suspected cases collected in the period from 1998 to 1999. The isolated virus were identified as belonging to Enterovirus genus and specifically to echovirus serotype 30 (EV-30), using immunological techniques as Indirect Immunofluorescense Assay (IFA) and Neutralization (Nt) and by molecular technique of Reverse Transcription Polymerase Chain Reaction (RT-PCR) directed to the 5'NTR and VP1 regions of viral genome. The results obtained contributed with to the surveillance system of São Paulo State and they are important due to a shortage of epidemiological data about the circulation of EV-30 associated to aseptic meningitis in this region of the world.

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“…Since the first descriptive outbreak of aseptic meningitis due to this virus in Scotland in 1959 (5), it has become clear that infection with this virus is unusually prevalent throughout the United States and European countries (6,9,11,25,26,28). Antigenic heterogeniety has been reported among strains isolated at different times and places (6,14,29).…”

mentioning

confidence: 99%

Characterization of an Echovirus Type 30 Variant Isolated from Patients with Aseptic Meningitis

Matsumoto

Yokochi

Matsuda³

et al. 1986

Microbiology and Immunology

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An outbreak of echovirus type 30 infection associated with aseptic meningitis occurred among newborn babies in a hospital neonatal room at Fukui City in 1983. The isolated virus was identified as an antigenic variant of echovirus type 30 by cross-neutralization tests with antisera against the prototype Bastianni strain and the present isolate. Western blot analysis demonstrated that the antigeni determinants responsible for virus neutralization, some of which were type specif and others strain specific, were present on the capsid protein VP1. The curren strain was more thermoresistant than the prototype, suggesting some alterations in virus structural proteins.

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The Antigenic Heterogeneity of Type 30 Echoviruses12

Cited by 19 publications

References 0 publications

Molecular Epidemiology of Echovirus 30: Temporal Circulation and Prevalence of Single Lineages

Molecular Epidemiology of Echovirus 30: Temporal Circulation and Prevalence of Single Lineages

Aseptic meningitis by echovirus 30 in São Paulo state, Brazil

Characterization of an Echovirus Type 30 Variant Isolated from Patients with Aseptic Meningitis

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