Characterization of an Echovirus Type 30 Variant Isolated from Patients with Aseptic Meningitis

Matsumoto, Kazuo; Yokochi, Takashi; Matsuda, Susumu; Itoi, Yoshiyuki; Kimura, Yoshinobu

doi:10.1111/j.1348-0421.1986.tb00950.x

Cited by 9 publications

(2 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1 A new variant of echovirus type 30 that is also associated with viral meningitis has been described. 5 Arboviruses account for about 5% of all cases of aseptic meningitis in North America. St Louis encephalitis virus is the most common arboviral cause.…”

Section: Causes Of Viral Meningitismentioning

confidence: 99%

Viral meningitis

Maxson¹,

Jacobs²

1993

Postgraduate Medicine

View full text Add to dashboard Cite

Viral meningitis is a common illness among children and is most often caused by enteroviruses. Diagnosis is made on the basis of clinical findings and analysis of the cerebrospinal fluid. Important conditions to consider in diagnosis are partially treated bacterial meningitis, tuberculous meningitis, fungal meningitis, other infections, and central nervous system tumor. Ruling out treatable causes early is important. Treatment of enteroviral meningitis is supportive, and the prognosis is usually very good, although significant neurologic sequelae have been reported.

show abstract

Section: Causes Of Viral Meningitismentioning

confidence: 99%

Viral meningitis

Maxson¹,

Jacobs²

1993

Postgraduate Medicine

View full text Add to dashboard Cite

show abstract

“…During the epidemic we isolated echovirus type 30 from patients with aseptic meningitis and the representative isolate was designated as the Fukui 609/83 strain (3). The strain was identified as an antigenic variant having a strain-specific neutralization determinant(s) in addition to a type-specific antigen(s) in common with the prototype Bastianni strain.…”

mentioning

confidence: 99%

Seroepidemiological Studies of Echovirus Type 30 Infection

Matsumoto

Kobayashi²,

Kimura

1986

Microbiology and Immunology

Self Cite

View full text Add to dashboard Cite

A large outbreak of echovirus type 30 infection occurred throughout Japan in 1983. During the epidemic we isolated echovirus type 30 from patients with aseptic meningitis and the representative isolate was designated as the Fukui 609/83 strain (3). The strain was identified as an antigenic variant having a strain-specific neutralization determinant(s) in addition to a type-specific antigen(s) in common with the prototype Bastianni strain. Interestingly, most convalescent phase sera obtained from the patients with this virus infection showed a two-to fourfold higher neutralizing antibody response to the Fukui strain than to the Bastianni strain. This finding suggests that the outbreak in 1983 might have been caused by the Fukui strain rather than the prototype strain. To determine the prevalence of the two strains in detail, we carried out seroepidemiological studies retrospectively.The serum samples were obtained by random sampling from individuals visiting the Fukui Prefectural Central Hospital before and after the epidemic in 1983. Twenty serum samples were obtained from persons in each of the following age groups : 0-2, 3-5, 6-9, 10-12, 13-15, 16-19, 20-29, 30-39, 40-49, and 50-59 years. Sera from babies less than 6 months old were not used. Sera collected in 1977 and 1979 were also examined, since an outbreak of this virus infection was reported to have occurred in Aichi Prefecture in 1978 (4). All sera were tested for neutralizing antibody against the Fukui 609/83 and the Bastianni strains of echovirus type 30. Neutralization tests were carried out by the microtiter method after inactivation of the sera at 56 C for 30 min. A 0.025-ml volume of a suspension containing 100 TCID50 of the virus was mixed with an equal volume of serial twofold diluted serum and incubated at 37 C for 90 min. The mixtures were then kept at 4 C overnight and inoculated onto FL cell monolayers. The neutralizing antibody titer was expressed as the reciprocal of the highest dilution of serum inhibiting viral cytopathic effect. Figure 1 shows the prevalence of antibody to echovirus type 30 in the different age groups before and after the epidemic. In September 1977 no children younger than 15 years old were seropositive (Fig. 1A). However, two years later seropositive children appeared, and 15 % of the children 6 to 9 years of age showed high neutralizing antibody titers (Fig. 1B), suggesting that sporadic infection with echovirus type 30 did occur during the two years although an attempt to isolate this virus was unsuccessful. On the other hand, adults had a high rate of seropositivity in 935

show abstract

Isolation and preliminary characterization of antigenic variant of echovirus type 11

Matsumoto

Kobayashi²,

Kimura

1990

Journal of Medical Virology

Self Cite

View full text Add to dashboard Cite

Nosocomial infection with echovirus type 11 resulting in aseptic meningitis occurred among newborn babies in a hospital neonatal room at Fukui city. The virus was identified as a variant of echovirus type 11 by cross-neutralization tests with antisera against the prototype Gregory strain and the current Fukui isolate. Fukui isolates expressed strain specific antigen(s) in addition to type specific common antigen(s), but lacked a certain antigen(s) which was present in the prototype strain. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of virus polypeptides revealed that the capsid proteins VP2 and VP3 of Fukui strain migrated more rapidly than the Gregory strain, while both strains had the same migration pattern of VP1 protein on which the antigenic determinants responsible for virus neutralization were present. The current strain produced large plaques and was more thermoresistant, suggesting some alterations in the structural proteins of the virus.

show abstract

Characterization of an Echovirus Type 30 Variant Isolated from Patients with Aseptic Meningitis

Cited by 9 publications

References 29 publications

Viral meningitis

Viral meningitis

Seroepidemiological Studies of Echovirus Type 30 Infection

Isolation and preliminary characterization of antigenic variant of echovirus type 11

Contact Info

Product

Resources

About