The antigenic components of Erysipelothrix rhusiopathiae II. Purification and chemical characterization of a type-specific antigen

Kalf, George F.; White, Thomas G.

doi:10.1016/0003-9861(63)90317-5

Cited by 21 publications

(6 citation statements)

References 17 publications

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“…Peptidoglycan antigens form the basis for the classification of this organism into serotypes and subtypes, and at least 22 different serotypes have been identified (11,17,22,32,33). Most of the isolates from diseased animals belong to serotype 1 or 2 (32).…”

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confidence: 99%

Cloning and expression in Escherichia coli of a protective antigen of Erysipelothrix rhusiopathiae

Galán

Timoney

1990

Infect Immun

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Erysipelothrix rhusiopathiae is a primary pathogen of swine and turkeys and sporadic cause of disease in a variety of other hosts, including humans. A genomic library of the highly virulent strain of E. rhusiopathiae E1-6P was constructed in the expression-cloning vector Xgtll and screened with serum from a pig convalescent from an E. rhusiopathiae experimental infection. Immunoreactive clones were screened for their ability to protectively immunized mice. Two clones, AgtlllersA and Agtll/ersB, were obtained that protected mice against challenge with E. rhusiopathiae E1-6P. Antisera against the recombinant clones reacted with polypeptides of molecular weights 66,000, 64,000, and 43,000 in detergent-solubilized surface antigen preparations and whole-cell lysates of E. rhusiopathiae. These polypeptides were also the major antigens recognized by convalescent pig serum when reacted with the same preparations. Western immunoblot and Southern blot analysis revealed that the cloned genes and gene products were present in all of the E. rhusiopathiae strains tested.

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confidence: 99%

Cloning and expression in Escherichia coli of a protective antigen of Erysipelothrix rhusiopathiae

Galán

Timoney

1990

Infect Immun

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“…The existence of a species-specific proteinaceous 'protective antigen' distinct from type-specific polysaccharide antigens has been repeatedly mentioned by earlier investigators [18,32,42]. A generic antigen extracted from cells of E. rhusiopathiae by acetone and purified by ion exchange chromatography [20,43] was composed of four serologically identical antigens of which one fourth was larger than 200 kDa as measured by analytical centrifugation [44]. White and Verwey [22] examined crude culture supernatants of E. rhusiopathiae and.…”

Section: Discussionmentioning

confidence: 99%

“…However, titres of these antibodies do not always correlate with protection [17], most probably because in vitro assays do not distinguish between protective antibodies and antibodies to non-protective, type-specific polysaccharide antigens. Non-protective E. rhusiopathiae antigens in hot acid extracts are predominantly polysaccharides and have been used to classify strains into 22 different serotypes [5,[18][19][20][21]. Immunity to E. rhusiopathiae is stimulated by vaccination with attenuated or formalized cells, by culture supernatant [22,23] and by crude extracts obtained by ultrasound or 10 mm NaOH [24].…”

Section: Introductionmentioning

confidence: 99%

Characterization of a protective protein antigen ofErysipelothrix rhusiopathiae

et al. 1991

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Although vaccination is widely practiced against infection by Erysipelothrix rhusiopathiae in pigs and turkeys, the protective antigen(s) involved have not been fully characterized or purified to homogeneity. Antigens of E. rhusiopathiae strain T28, serotype 2b, and of FRANKFURT XI, serotype N, in culture supernatant and in extracts made with hot acid, 10 mM NaOH, ultrasound or EDTA were compared by SDS-PAGE and immunoblotting and in a mouse protection test. EDTA and 10 mM NaOH yielded highly protective extracts; culture supernatant was less protective and ultrasonic or hot acid extracts stimulated little or no protection in mice. Protective antisera from swine, horses and mice recognized prominent bands of molecular mass (m.m.) of 66-64 and 40-39 kDa in EDTA and 10 mM NaOH extracts. Mice immunized with preparations of the 66-64 kDa band purified by preparative electrophoresis were protected. Both antigens were trypsin sensitive, contained no detectable polysaccharide, and showed a marked tendency to aggregate in the absence of SDS.

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“…Makino et al (7) established a PCR system using highly specific primers, MO101 and MO102, for detection of Erysipelothrix species. On the basis of studies with DNA-DNA hybridization and with MO101 and MO102, the genus Erysipelothrix was reported to be divided into four species, E. rhusiopathiae (serovars 1a, 1b, 2, 4, 5, 6, 8, 9, 11, 12, 15, 16, 17, 19, 21, and N), E. tonsillarum (serovars 3, 7, 10, 14, 20, 22, and 23), E. rhusiopathiae serovar 13, and E. rhusiopathiae serovar 18; and those four species could not be distinguished from each other by PCR (4,11,12).…”

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Direct and Rapid Detection by PCR of Erysipelothrix sp. DNAs Prepared from Bacterial Strains and Animal Tissues

et al. 1999

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A PCR method for rapid screening of Erysipelothrix spp. in the slaughterhouse was carried out by using four species-specific sets of oligonucleotide primers after initial amplification with the primer set MO101-MO102, which amplifies the 16S rRNA sequences of all four Erysipelothrix species. The DNA sequences coding for the rRNA gene cluster, including 16S rRNA, 23S rRNA, and the noncoding region downstream of 5S rRNA, were determined in order to design primers for the species-specific PCR detection system. The homology among the 4.5-kb DNA sequences of the rRNA genes ofErysipelothrix rhusiopathiae serovar 2 (DNA Data Bank of Japan accession no. AB019247), E. tonsillarum serovar 7 (accession no. AB019248), E. rhusiopathiae serovar 13 (accession no. AB019249), and E. rhusiopathiae serovar 18 (accession no. AB019250) ranged from 96.0 to 98.4%. The PCR amplifications were specific and were able to distinguish the DNAs from each of the four Erysipelothrix species. The results of PCR tests performed directly with tissue specimens from diseased animals were compared with the results of cultivation tests, and the PCR tests were completed within 5 h. The test with this species-specific system based on PCR amplification with the DNA sequences coding for the rRNA gene cluster was an accurate, easy-to-read screening method for rapid diagnosis of Erysipelothrix sp. infection in the slaughterhouse.

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The antigenic components of Erysipelothrix rhusiopathiae II. Purification and chemical characterization of a type-specific antigen

Cited by 21 publications

References 17 publications

Cloning and expression in Escherichia coli of a protective antigen of Erysipelothrix rhusiopathiae

Cloning and expression in Escherichia coli of a protective antigen of Erysipelothrix rhusiopathiae

Characterization of a protective protein antigen ofErysipelothrix rhusiopathiae

Direct and Rapid Detection by PCR of Erysipelothrix sp. DNAs Prepared from Bacterial Strains and Animal Tissues

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