The Antigenic and Allergenic Properties of Botrytis Cinerea

Kauffman, Hf; Heide, S. van der; Beaumont, F; Vries, K. de

doi:10.1007/978-1-4613-0773-0_33

Cited by 3 publications

(13 citation statements)

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“…In agreement with previously described results [3,7,8,10] I found a good correlation between DID and ELISA among sera that contain a high level of anti body to A. fumigatus. Antibodies in these sera react with several (up to 20) antigens as detected by immunoblot analyses.…”

Section: Discussionsupporting

confidence: 79%

“…This latter point is also stressed by the results obtained with precipitin-negative sera. The percent age of these sera with elevated IgG titers was almost equal to that reported by Kauffman et al [7]. How ever, in a considerable number of these sera, ELISA results were positive for IgM only.…”

Section: Discussionsupporting

confidence: 63%

“…Most widely used is the en zyme-linked immunosorbent assay (ELISA) [3][4][5][6]. Numerous studies have been performed in which the results obtained in DID were compared with IgG ti ters measured in ELISA [3][4][5][7][8][9][10]. Although there is a correlation between these methods, precipitin-nega tive sera may contain increased ELISA titers for IgG whereas, on the other hand, there are precipitin-posi tive sera that are negative for IgG in ELISA.…”

Section: Introductionmentioning

confidence: 99%

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Detection of Antibodies against Aspergillus fumigatus: Comparison between Double Immunodiffusion, ELISA and Immunoblot Analysis

Brouwer¹

1988

Int Arch Allergy Immunol

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The performance of ELISA to detect IgG and IgM antibodies to Aspergillus fumigatus has been evaluated in strongly precipitin-positive, weakly precipitin-positive and precipitin-negative patient sera, with immunoblot analysis as the confirmatory test. All strongly precipitin-positive sera contained increased IgG titers and showed clearly positive immunoblot patterns. Most of the weakly precipitin-positive sera contained ELISA titers within the normal range established with sera of healthy blood donors and showed normal immunoblot patterns. Increased titers of IgG and/or IgM were measured in one-sixth of the precipitin-negative patient sera. Immunoblot analysis confirmed the presence of antibodies to A. fumigatus in 55% of the precipitin-negative sera with increased antibody titers. ELISAs for A. fumigatus-specific IgG and IgM are sensitive tests for screening of patient sera. However, positive results with ELISA should be confirmed by means of immunoblot analysis.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionsupporting

confidence: 63%

Section: Introductionmentioning

confidence: 99%

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Detection of Antibodies against Aspergillus fumigatus: Comparison between Double Immunodiffusion, ELISA and Immunoblot Analysis

Brouwer¹

1988

Int Arch Allergy Immunol

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“…The mould was grown on a Sabouraud-2% glucose medium, un der similar conditions as described for A. fumigatus [8,9], except the temperature which was kept at 20°C. The pH of the culture me dium was regularly measured as a parameter of the state of growth of the microorganism [8,9,II,12].…”

Section: Cultivation Of B Cinereamentioning

confidence: 99%

“…In this study we investigated the antibody bind ing properties of extracts of B. cinerea using sera of patients with positive skin tests for this mould. Recent studies from our department have shown that both IgE and IgG binding properties of different moulds are continuously changing during cultivation [8][9][10], The IgE and IgG binding characteristics of B. cinerea were studied in different extracts isolated after increas ing periods of cultivation. In addition, the frequency of IgE binding to B. cinerea extract was studied with a group of sera with elevated IgE antibodies against A. fumigatus.…”

Section: Introductionmentioning

confidence: 99%

Botrytis cinerea: A Study of the Immunological Properties during Growth

Kauffman¹,

Heide²,

Vries³

1987

Int Arch Allergy Immunol

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When growing Botrytis cinerea in Sabouraud medium, different phases of growth could be recognized using pH, mycelium yield, and culture filtrate antigens as parameters of growth. Immunological characteristics of the different phases (early, intermediate, and late) of growth were measured using IgE and IgG binding by enzyme allergosorbent test and enzyme-linked immunosorbent assay, respectively, and quantities of precipitating components (double immunodiffusion and rocket immunoelectrophoresis). The number of precipitating components was maximal in culture filtrate extracts during late phase (III) of growth. In contrast, both IgE and IgG binding were already maximal in the early phase of growth (9 days of cultivation). In a group of patients with elevated IgE antibodies against Aspergillus fumigatus, about half of them also showed elevated IgE antibodies against B. cinerea. This indicates that multiple exposition is a cause of multiple sensitization; however, cross-reactivity in some of these sera cannot be excluded.

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The 18-kilodalton antigen secreted by Aspergillus fumigatus

et al. 1991

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One of the major antigens secreted in vitro by Aspergilus fumigatus is an 18-kDa basic protein which has been purified by cation-exchange chromatography. It is recognized by sera from aspergilloma patients. It is also the major circulating antigen found in urine of patients with invasive aspergillosis. Our results indicated that this antigen has potential for the diagnosis of both aspergilloma and invasive aspergillosis.

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The Antigenic and Allergenic Properties of Botrytis Cinerea

Cited by 3 publications

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Detection of Antibodies against <i>Aspergillus fumigatus</i>: Comparison between Double Immunodiffusion, ELISA and Immunoblot Analysis

Detection of Antibodies against <i>Aspergillus fumigatus</i>: Comparison between Double Immunodiffusion, ELISA and Immunoblot Analysis

<i>Botrytis cinerea:</i> A Study of the Immunological Properties during Growth

The 18-kilodalton antigen secreted by Aspergillus fumigatus

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