The 18-kilodalton antigen secreted by Aspergillus fumigatus

Latgé, Jean‐Paul; Moutaouakil, M.; Debeaupuis, J P; Bouchara, Jean Philippe; Haynes, Ken; Prévost, Marie‐Christine

doi:10.1128/iai.59.8.2586-2594.1991

Cited by 102 publications

(50 citation statements)

References 32 publications

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“…The Aspergillus lateral flow assay may be considered in the next evaluation by the EORTC/MSG consensus group and this could provide a new impetus to define further protein antigens for diagnosis of Aspergillus infections. First candidate antigens have already been detected in clinical samples [98,99] or have been shown to induce specific antibody responses in patients [100][101][102][103].…”

Section: Detection Of Antigensmentioning

confidence: 99%

Immunoproteomics of Aspergillus for the development of biomarkers and immunotherapies

Kniemeyer

Ebel²,

Krüger

et al. 2016

Proteomics Clinical Apps

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Filamentous fungi of the genus Aspergillus play significant roles as pathogens causing superficial and invasive infections as well as allergic reactions in humans. Particularly invasive mycoses caused by Aspergillus species are characterized by high mortality rates due to difficult diagnosis and insufficient antifungal therapy. The application of immunoproteomic approaches has a great potential to identify new targets for the diagnosis, therapy, and vaccine development of diseases caused by Aspergillus species. Serological proteome analyses (SERPA) that combine 2D electrophoresis with Western blotting are still one of the most popular techniques for the identification of antigenic proteins. However, recently a growing number of approaches have been developed to identify proteins, which either provoke an antibody response or which represent targets of T-cell immunity in patients with allergy or fungal infections. Here, we review advances in the studies of immune responses against pathogenic Aspergilli as well as the current status of diagnosis and immunotherapy of Aspergillus infections.

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Section: Detection Of Antigensmentioning

confidence: 99%

Immunoproteomics of Aspergillus for the development of biomarkers and immunotherapies

Kniemeyer

Ebel²,

Krüger

et al. 2016

Proteomics Clinical Apps

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“…BG is one of the main cell-wall polysaccharide components of Aspergillus [135]; it can be colourimetrically detected and is useful in diagnosis. This component is specific for fungi except for zygomycetes and cryptococci [136].…”

Section: Non-invasive Proceduresmentioning

confidence: 99%

Update on invasive aspergillosis: clinical and diagnostic aspects

Muñóz

Guinea

Bouza

2006

Clinical Microbiology and Infection

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A B S T R A C TApergillus is a ubiquitious mould that can cause a wide variety of clinical syndromes ranging from mere colonisation to fulminant invasive disease. Invasive aspergillosis (IA) is the most severe presentation of aspergillosis. The lung is usually the portal of entry, from which the pathogen may disseminate to almost any organ, often the brain and skin. The diagnosis remains a significant challenge. It is usually based on a combination of compatible clinical findings in a patient with risk-factors and isolation of the microorganism, radiological data, serological detection of antibodies or antigens, or histopathological evidence of invasion. Chest radiographic findings in patients with pulmonary Aspergillus may initially be normal in up to 10% of cases. Computed tomography scanning is probably the most useful imaging technique for the diagnosis of IA, since it may reveal lung lesions up to 5 days earlier than would radiograph techniques simply. Currently available laboratory diagnostic methods include several techniques: histopathological evidence of invasion; isolation of the microorganism and direct microscopy from clinical samples and non-invasive procedures (serological detection of antigens or nucleic material of Aspergillus; detection of antibodies). The histological diagnosis of IA requires the presence of invasion by fungus of the Aspergillus species. The truth is that, if no other variables are considered, the positive predictive value is very low, and most of the isolates of A. fumigatus do not represent proven or probable infection. Several molecules could be used as markers of infection, but two of them are of special interest: Aspergillus galactomannan (GM) and (1 fi 3)-b-glucan (BG). GM has a high specificity (above 85%) and a reported sensitivity that varies widely (between 30% and 100%). BG, a main cell wall polysaccharide component of Aspergillus, can be colourimetrically detected and is useful in diagnosis, with a sensitivity ranging from 50% to 87.5%. A specific Aspergillus PCR assay has also been used in the diagnosis of IA and has shown very good results, with a sensitivity and specificity of 100% and 89%, respectively.

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“…Water-soluble ethanol-precipitated (WSEP) secretory antigens were prepared from A. fumigatus strain CBS 144.89 by ethanol precipitation of culture filtrate material as described elsewhere (20). Following the disintegration of mycelial mats of A. fumigatus strain ATCC 42202 in an X-press operated at a maximal force of 200 MPa at -3O"C, preparations of water-soluble somatic hyphal (WSSH) antigens were prepared as described previously (9).…”

Section: Fungal Strains and Preparations Of Antigensmentioning

confidence: 99%

An analysis of antibodies against Aspergillus jumigatus in bovine serum by iminunoblotting and enzyme‐linked immunosorbent assays

Jensen

Latgé

1995

APMIS

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Serum samples from 20 cows with spontaneously acquired systemic aspergillosis and serum collected consecutively from a cow with experimentally induced aspergillosis were assayed for antibodies against Aspergillus fumigatus in immunoblotting and enzyme‐linked immunosorbent assays. Purified antigens (18 kDa protein and 20 kDa galactomannan) from A. fumigatus as well as complex preparations of metabolic and somatic antigens were used. Antibodies against A. fumigatus were found in both infected and non‐infected cows. High titers and especially an increase in titer in consecutive samples signified infection. Reactivity with the 22 and 32 kDa antigens in preparations of exocellular antigens and the 19, 20, and 39 kDa antigens from intracellular antigens was seen with sera from infected cows only, and is therefore indicative of infection.

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The 18-kilodalton antigen secreted by Aspergillus fumigatus

Cited by 102 publications

References 32 publications

Immunoproteomics of Aspergillus for the development of biomarkers and immunotherapies

Immunoproteomics of Aspergillus for the development of biomarkers and immunotherapies

Update on invasive aspergillosis: clinical and diagnostic aspects

An analysis of antibodies against Aspergillus jumigatus in bovine serum by iminunoblotting and enzyme‐linked immunosorbent assays

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