The Antigen Involved in Immune Adherence with Stratum corneum and Human Serum

Krogh, H.K.

doi:10.1159/000230261

Cited by 23 publications

(38 citation statements)

References 5 publications

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“…It can thus be concluded that the antigens form constituent parts of callus. This is consistent with previous findings based on the use of the immune adherence test with sections of human skin [5],…”

Section: Discussionsupporting

confidence: 93%

“…This antigen was destroyed by oxidation with periodate. This is consistent with earlier suggestions that at least one of the antigens in volved is of carbohydrate nature [5]. In addition, the PW water phase contains another antigen which resisted treatment with periodate.…”

Section: Discussionsupporting

confidence: 92%

“…Microtome sections of frozen human skin from adults were obtained and processed as previously reported [6,7], Extraction of tissue. Lyophilized tissue was extracted with PW as earlier de scribed [5]. In short, phosphate-buffered saline pH 7.2 (buffered saline) and 90% phenol, 15 ml of each were added to 1 g of lyophilized tissue.…”

Section: Methodsmentioning

confidence: 99%

“…Originally, these antibodies were demonstrated using the immune adherence test with microtome sections of human skin [7], and later by fluorescent antibody and indirect haemag glutination techniques [5,10]. The antigen used for sensitization of eryth rocytes was prepared by extraction of homogenates of callus with phenol water (PW) and recovered from the water phase.…”

mentioning

confidence: 99%

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Heterogeneity of Antigen from Callus of Man

Mæland¹,

Krogh²,

Tönder³

1974

Int Arch Allergy Immunol

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The question of whether one or more antigens are involved in the reaction between antibodies in human serum and callus of man was investigated. Callus was collected from an individual with congenital hyperkeratosis. Preparations were obtained by extraction of homogenated callus with (1) phenol water (PW), and (2) the combined use of trypsin digestion and phenol water extraction (TPW). The phenol and water phases were studied using indirect haemagglutination technique. All preparations sensitized erythrocytes for agglutination by human serum except for the phenol phase of the PW preparation. Absorption and inhibition experiments showed that one antigen present in the TPW water phase was also present in the PW water phase. In addition, the PW water phase contained another antigen. In the phenol phase of the TPW preparation a third antigen was demonstrated. Serological cross-reactivity between the antigens and bacteria isolated from callus was not demonstrated. The results of immune adherence test with sections of human skin and unabsorbed or absorbed human serum indicated that the two water phase antigens are present in normal human skin.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 92%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Heterogeneity of Antigen from Callus of Man

Mæland¹,

Krogh²,

Tönder³

1974

Int Arch Allergy Immunol

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show abstract

“…

Antibodies in normal sera from human adults to an intercellular antigen of stratum corncum have been reported in a previous publi cation [15]. These antibodies belong to the yM and yG classes of immunoglobulins [13].

…”

mentioning

confidence: 73%