The anticonvulsant effect of sparteine on pentylenetetrazole-induced seizures in rats: a behavioral, electroencephalographic, morphological and molecular study

Villalpando-Vargas, Fridha Viridiana; Medina‐Ceja, Laura; Santerre, Anne; Enciso-Madero, Edgar A

doi:10.1007/s10735-020-09899-0

Cited by 7 publications

(3 citation statements)

References 46 publications

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“…The resulting cDNAs were quantified by UV-spectrophotometry and used for qPCR. The following primers were used: GPER forward, 5′-CTTCTGCCATGCCACGCT-3′, and reverse, 5′-ACATCTGACTGCTCCGTGCTG-3′ ( 20 ); and GAPDH forward, 5′-GCTGGTCATCAACGGGAAAC-3′ and GAPDH reverse, 5′-GACTCCACGACATACTCAGCACC-3′ ( 21 ). The primers were synthesized by Integrated DNA Technologies, Inc. qPCR was performed using Maxima SYBR-Green/ROX qPCR master mix (cat.…”

Section: Methodsmentioning

confidence: 99%

Proliferation and apoptosis regulation by G protein‑coupled estrogen receptor in glioblastoma C6 cells

et al. 2022

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Glioblastoma is the most frequent primary tumor in the human brain. Glioblastoma cells express aromatase and the classic estrogen receptors ERα and ERβ and can produce estrogens that promote tumor growth. The membrane G protein-coupled estrogen receptor (GPER) also plays a significant role in numerous types of cancer; its participation in glioblastoma tumor development is not entirely known. The present study investigated the effect of the agonists [17β-estradiol (E2) and G1] and antagonist (G15) of GPER on proliferation and apoptosis of C6 glioblastoma cells. GPER expression was evaluated by immunofluorescence, western blotting and reverse transcription-quantitative PCR. Cell proliferation was determined using Ki67 immunopositivity. Cell viability was examined using the MTT assay and apoptosis using caspase-3 immunostaining and ELISA. C6 cells express GPER, and the immunopositivity increased after exposure to E2, G1, or their combination. GPER protein expression increased after treatment with E2 combined with G1. However, GPER mRNA expression decreased in treated cells compared with control. The percentage of Ki67 immunopositive C6 cells increased under the effect of E2 in combination with G1 or G1 alone. G15 significantly reduced Ki67 immunopositivity. Pearson's correlation analysis revealed a positive relationship between GPER and Ki67 immunopositivity across the study conditions. Additionally, the MTT assay showed a significant reduction in C6 cell viability after G15 treatment, alone or in combination with G1. The exposure to G15 increased the percentage of caspase-3 immunopositivity cells and caspase-3 levels. Pearson's correlation analysis demonstrated a negative correlation between GPER and caspase-3 immunopositivity across the study conditions. Glioblastoma C6 cells express GPER, and this receptor modulates cell proliferation and apoptosis. The GPER agonists E2 and G1 favored cell proliferation; meanwhile, the antagonist G15 reduced cell proliferation, viability and favored apoptosis. Therefore, GPER may be used as a biomarker of glioblastoma and as a target to develop new therapeutic strategies for glioblastoma treatment.

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Section: Methodsmentioning

confidence: 99%

Proliferation and apoptosis regulation by G protein‑coupled estrogen receptor in glioblastoma C6 cells

et al. 2022

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“…Cytisus scoparius (UR 2) and the oil of Linum usitatissimum (UR 2) were also used to treat dogs with convulsions or hysteria. C. scoparius contains sparteine, a quinolizidine alkaloid which acts as an anticonvulsant (Villalpando-Vargas et al 2020). Linseed oil is rich in Omega-3 and alpha-linolenic acid and plays a role in the management of seizures (Elkordy et al 2021, Tanna et al 2012.…”

Section: Antiepileptics and Other Nervous System Drugsmentioning

confidence: 99%

Ethnoveterinary data in Britain and Ireland: can native herbal medicine promote animal health?

Milliken

2023

Ethnobot. Res. App.

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Background: The use of plants, and occasionally fungi, to treat and cure animals or to supplement their feeding for livestock was widespread, globally. In some parts of the world this remains a key practice, but there is increasing use of veterinary pharmaceuticals which can have a negative effect on the wider environment. Meanwhile, traditional knowledge is being lost at a great rate, before it has been properly recorded and analyzed.Objective: This research analyses current and past ethnoveterinary use in Britain and Ireland and analyses the data within the context of medicinal uses, pharmacology, and other ethnoveterinary information collected in Europe.Methods: Ethnoveterinary data, collected from citizen science and literature, was analyzed by regional distribution (counties), use records (UR), and ATCvet classification.Results: A broad survey of the plants and fungi traditionally used to treat animals in the Britain and Ireland revealed 198 medicinal plants, principally for farm animals. This was a preliminary investigation, and although requests for current information were sent out in several formats though citizen science, the responses were relatively limited compared with other in situ ethnoveterinary surveys in other European countries. This may be partly due to the methodology, but probably also due to increased availability of modern veterinary medicines, the loss of traditional knowledge transfer between the generations, and concerns about animal safety, toxicity, and effectiveness with phytotherapy. The information reported here was compared with data collected in other countries and their known pharmacology. Some of the plant species cited are used more broadly within Europe for the same medicinal purposes, but in other cases the use appears to be restricted to Britain and Ireland. Conclusions:The information on ethnoveterinary uses recorded in this paper could assist with the development of novel biodegradable drugs and feed supplements for future animal management in a changing climate. With the increasing use of herbal lays and biodiversity grazing, and serious concerns about the over-use of veterinary medicines such as antibiotics and anthelmintics, and associated negative impacts on the environment and biodiversity, developing novel plant-based remedies may help to address some of the challenges. Further research on traditional ethnoveterinary knowledge in Britain and Ireland is timely, before the information has disappeared.

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“…Intriguingly, there is no obvious seizure phenotype in global M 2 knockout mice [33]. Additionally, sparteine, an anticonvulsant drug, increasing the hippocampal M 4 receptor expression on PTZ-induced seizures, indicating that the M 4 receptor may be also critical for seizures [41].…”

Section: Machrs and Epilepsymentioning

confidence: 99%

Cholinergic Signaling, Neural Excitability, and Epilepsy

et al. 2021

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Epilepsy is a common brain disorder characterized by recurrent epileptic seizures with neuronal hyperexcitability. Apart from the classical imbalance between excitatory glutamatergic transmission and inhibitory γ-aminobutyric acidergic transmission, cumulative evidence suggest that cholinergic signaling is crucially involved in the modulation of neural excitability and epilepsy. In this review, we briefly describe the distribution of cholinergic neurons, muscarinic, and nicotinic receptors in the central nervous system and their relationship with neural excitability. Then, we summarize the findings from experimental and clinical research on the role of cholinergic signaling in epilepsy. Furthermore, we provide some perspectives on future investigation to reveal the precise role of the cholinergic system in epilepsy.

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The anticonvulsant effect of sparteine on pentylenetetrazole-induced seizures in rats: a behavioral, electroencephalographic, morphological and molecular study

Cited by 7 publications

References 46 publications

Proliferation and apoptosis regulation by G protein‑coupled estrogen receptor in glioblastoma C6 cells

Proliferation and apoptosis regulation by G protein‑coupled estrogen receptor in glioblastoma C6 cells

Ethnoveterinary data in Britain and Ireland: can native herbal medicine promote animal health?

Cholinergic Signaling, Neural Excitability, and Epilepsy

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