The Anticancer Effect of Cannabinoid 2 Agonist L-759,633 on C6 and SH-SY5Y Cell Lines

Joha, Zıad; Yulak, Fatih; Öztürk, Ayşegül; Şahin, Bilal; Yıldırım, Seda

doi:10.51972/tfsd.929180

Cited by 4 publications

(2 citation statements)

References 45 publications

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“…The cell viability was expressed as a percentage compared to the control group (100 percent viability). (Joha et al, 2021) The magnetic field effect of free cisplatin and cisplatin‐loaded magnetic triple combinations in the MCF‐7 cell line was investigated by 10 and 20 min of external alternating current magnetic field exposure.…”

Section: Methodsmentioning

confidence: 99%

Magnetic chitosan oligomer‐sulfonate‐stearic acid triple combination as cisplatin carrier for site‐specific targeted on MCF‐7 cancer cells: Preparation, characterization and in vitro experiments

Akkaya,

Nazlim

2023

Chem Biol Drug Des

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In this study, a new amphiphilic target‐specific adsorbent, chitosan oligomer‐sulfonate‐stearic acid triple combination (S‐Cho‐SA), and magnetic chitosan oligomer‐sulfonate‐stearic acid triple combination (M‐S‐Cho‐SA) by oleic acid (OA)‐modified Fe3O4 via hydrophobic interaction are fabricated. By modifying the nanoparticle surfaces and having the ability to magnetically allow the target region, these particles attract attention as important particles used in targeting mechanisms in cancer therapy. With magnetic nanoparticles and an external magnetic field, it is possible to transport therapeutic agents to the target site and keep them in the desired effect zone for a longer period of time. These new adsorbents are characterized by scanning electron microscopy (SEM), attenuated total reflection Fourier transform infrared (ATR FT‐IR) spectroscopy, nuclear magnetic resonance (NMR), X‐ray diffraction (XRD), vibrating sample magnetometer (VSM), and thermogravimetric analysis (TG/DTA). After chemical characterization, it is complexed with cisplatin (CDDP). The magnetic adsorbents were loaded with high efficiency (>50%), and the release experiments exhibited that cisplatin is released more at pH 4.5 compared with pH 7.4 at 37°C. It showed better drug release results under a magnetic field for magnetic adsorbents (36% for pH 4.5 and 3.6% for pH 7.4). The biocompatibility of the prepared adsorbents was demonstrated via the XTT assay in MCF‐7 cell lines. The results also exhibited that S‐Cho‐SA and M‐S‐Cho‐SA were biocompatible, and free cisplatin and cisplatin‐complexed adsorbents showed an antiproliferative effect. The results showed that these new cisplatin‐loaded (M‐S‐Cho‐SA) nanoparticles are good candidates for thermotherapy in cancer treatment in the future, as they can provide selectivity by site‐specific targeting and hold onto an alternative magnetic field due to the magnetic nature of the nanoparticles.

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Section: Methodsmentioning

confidence: 99%

Magnetic chitosan oligomer‐sulfonate‐stearic acid triple combination as cisplatin carrier for site‐specific targeted on MCF‐7 cancer cells: Preparation, characterization and in vitro experiments

Akkaya,

Nazlim

2023

Chem Biol Drug Des

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“…After 4-hour incubation, the cells were shaken and the absorbance was measured at 450 nm using a microplate reader (Thermo Fisher Scienti c, Altrincham, United Kingdom). Cell viability was assessed as a percentage of live cells versus control cells after each experiment was performed three times (10,11).…”

Section: Cell Viability Testmentioning

confidence: 99%

Anticancer activity of lycopene in HT-29 colon cancer cell line

et al. 2023

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Purpose: An inverse association between serum lycopene levels and risk of cancers has been pointed out by many prospective and retrospective epidemiological studies which prompted more studies to be performed on animal models and cell cultures in order to test this hypothesis. The aim of the present study was to evaluate the antiproliferative and pro-apoptotic effect of lycopene on colon cancer HT-29 cell lines.Materials and Methods: The effect of lycopene on the viability of HT-29 cell line was investigated using XTT assay. the levels of BCL-2, cleaved caspase 3, BAX, cleaved PARP and 8-oxo-dG in lycopene-treated HT-29 cells were measured using ELISA. gamma-H2AX and cytochrome C expression was assessed semiquantitatively using immuno uorescence staining.Results: Lycopene at doses of 10 and 20 μM produced a signi cant antiproliferative effect on HT-29 cells compared to the control (p < 0.05). The IC50 value of lycopene in HT-29 cells was found to be 4.382 μM for 24 hours. Lycopene (4.382 μM) signi cantly elevated cleaved caspase 3 (p <0.01), BAX, and cleaved PARP, 8-oxo-dG levels (p<0.05). the levels of γ-H2AX foci are signi cantly higher while the levels of cytochrome-C are lower (p<0.05) in lycopene treated HT-29 cells. Conclusion:These results indicate that lycopene has an antiproliferative apoptotic and genotoxic effect on HT-29 colon cancer cell lines.

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Antimicrobially effective protein-loaded metal chelated chitosan composite

Akkaya,

Taşkın Kafa

2023

Journal of Polymer Engineering

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The immobilization of lysozyme onto a novel synthetic metal chelator composite based on chitosan/PAA-PMA (chitosan/acrylamide-maleic acid) and its effect on antimicrobial activity were the aim of the current study. The plain composite and the lysozyme immobilized composite were characterized according to scanning electron microscopy (SEM), Ultraviolet spectroscopy (UV), Fourier transform infrared (FT-IR), and X-ray diffraction (XRD) analysis. Furthermore, the activities of lysozyme and immobilized lysozyme were investigated, as well as their antimicrobial activity against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus faecalis, and Staphylococcus aureus, as well as their cytotoxic effects, which were both approximately greater than those of free lysozyme. The last step was cytotoxic studies on lysozyme, chitosan composite, and lysozyme-immobilized chitosan composite, which showed no cytotoxic effect. Several investigations have shown that metal chelators improve lysozyme’s antibacterial action. Two carboxylic acids containing maleic acid were used as a metal chelator in this study. Lysozyme was immobilized from a side other than carboxylic acids, so that carboxylic acid groups, which are metal chelating groups, do not prevent synergy by competing with metal binding at neutral pH. This enhanced antimicrobial activity.

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The Anticancer Effect of Cannabinoid 2 Agonist L-759,633 on C6 and SH-SY5Y Cell Lines

Cited by 4 publications

References 45 publications

Magnetic chitosan oligomer‐sulfonate‐stearic acid triple combination as cisplatin carrier for site‐specific targeted on MCF‐7 cancer cells: Preparation, characterization and in vitro experiments

Magnetic chitosan oligomer‐sulfonate‐stearic acid triple combination as cisplatin carrier for site‐specific targeted on MCF‐7 cancer cells: Preparation, characterization and in vitro experiments

Anticancer activity of lycopene in HT-29 colon cancer cell line

Antimicrobially effective protein-loaded metal chelated chitosan composite

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