Anticancer activity of lycopene in HT-29 colon cancer cell line

Abstract

Purpose: An inverse association between serum lycopene levels and risk of cancers has been pointed out by many prospective and retrospective epidemiological studies which prompted more studies to be performed on animal models and cell cultures in order to test this hypothesis. The aim of the present study was to evaluate the antiproliferative and pro-apoptotic effect of lycopene on colon cancer HT-29 cell lines.Materials and Methods: The effect of lycopene on the viability of HT-29 cell line was investigated using XTT assay. the levels of BCL-2, cleaved caspase 3, BAX, cleaved PARP and 8-oxo-dG in lycopene-treated HT-29 cells were measured using ELISA. gamma-H2AX and cytochrome C expression was assessed semiquantitatively using immuno uorescence staining.Results: Lycopene at doses of 10 and 20 μM produced a signi cant antiproliferative effect on HT-29 cells compared to the control (p < 0.05). The IC50 value of lycopene in HT-29 cells was found to be 4.382 μM for 24 hours. Lycopene (4.382 μM) signi cantly elevated cleaved caspase 3 (p <0.01), BAX, and cleaved PARP, 8-oxo-dG levels (p<0.05). the levels of γ-H2AX foci are signi cantly higher while the levels of cytochrome-C are lower (p<0.05) in lycopene treated HT-29 cells. Conclusion:These results indicate that lycopene has an antiproliferative apoptotic and genotoxic effect on HT-29 colon cancer cell lines.

Physicochemical properties, mechanism of action of lycopene and its application in poultry and ruminant production

“…Moreover, in “type II” cells, such as hepatocytes, the mitochondrial amplifying loop activates the effector caspase and then releases SMAC to downregulate XIAP expression to mitigate its mediated caspase inhibition ( 77 ). Previous studies have shown that lycopene reduced carcinogenesis by inhibiting the phosphatidyl-inositol 3-kinase/serine–threonine kinase (PI3K-AKT) signaling pathway, similarly, lycopene induced apoptosis by downregulating B, cell CLL/lymphoma-2 (BCL-2), and upregulating BAX ( 79 , 89 ). Previous records have shown that lycopene might increase the levels of Bax and E-cadherin and downregulate the levels of N-cadherin, phosphatidylinositol 3-kinase (p-PI3K), protein Kinase B (p-AKT), BCL-2, and phosphatidylinositol 3-kinases (PI3K)/AKT/mammalian target of rapamycin (p-m-TOR).…”

Lycopene: A Potent Antioxidant with Multiple Health Benefits

“…Furthermore, in animal models of hepatocellular carcinoma, administered lycopene suppressed the onset and development of cancer [ 175 ]. In human colorectal adenocarcinoma cell line, treatment with lycopene has shown to exhibit genotoxicity, antiproliferative, and apoptotic effects [ 176 ], a demonstration of its anticancer effects.…”

Recent Advances in Lycopene for Food Preservation and Shelf-Life Extension

“…In rodents with various clinical diseases, such as diabetes, colchicine exposure, high-fat diet, and aging, lycopene also enhances cognition and memory. In addition, lycopene protects against neurotoxicity induced by organic and inorganic pollutants, such as methylmercury, tert-butyl hydroperoxide, and cadmium [ 23 , 24 , 25 ]. This provides theoretical support for using lycopene in future clinical, therapeutic, and pharmaceutical applications.…”