The Antibody Content of Single Cells

Berenbaum, M. C.

doi:10.1136/jcp.11.6.543

Cited by 13 publications

(4 citation statements)

References 17 publications

(10 reference statements)

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“…It is of interest to compare these figures with the estimation for the amount of antibody stored by a plasma cell, calculated by Berenbaum (18). He estimated that a single plasma cell contained as much as 7.5 X 10-13 g of antibody, or one-fiftieth of the amount produced in a day in our estimate.…”

Section: Information Gained By Immunological Studies Of Single Lymphomentioning

confidence: 60%

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Elaboration of Antibodies by Single Cells

Nossal¹,

Mäkelä²

1962

Annu. Rev. Microbiol.

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Section: Information Gained By Immunological Studies Of Single Lymphomentioning

confidence: 60%

“…Use of radioactive antigen and autoradiography-This method, devel oped by Berenbaum (18,19 ), is similar in principle to the Coons direct technique. The antigen is labeIled with radioactive instead of fluorescent compounds.…”

Section: Histochemical Techniquesmentioning

confidence: 99%

Elaboration of Antibodies by Single Cells

Nossal¹,

Mäkelä²

1962

Annu. Rev. Microbiol.

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“…The size of the intracellular pool of gamma globulin has been measured in only a few studies. Berenbaum (22) estimated that normal lymphoid cells contain 0.75 pg/cell, while Salmon and Smith (23) found the myeloma cells contained 1.5 pg/cell. Gitlin and Sasaki (24) examined the immunoglobulin content of human tonsilar lymphoid cells and found between 0.2 and 6.0 pg/cell.…”

Section: Discussionmentioning

confidence: 99%

Independent Synthesis of Light and Heavy Chains

Laskov

Scharff

1974

The Journal of Experimental Medicine

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The rates of immunoglobulin synthesis have been examined in two MFC-11 cell lines which were independently adapted to tissue culture and in light-chain-producing variants derived from each of them. One cell line synthesized 2.5 pg immunoglobulin/cell/h, while the other synthesized 3.6 pg immunoglobulin/cell/h. The ratio of heavy and light chains in the two cell lines was approximately the same, and the size of the intracellular pool of immunoglobulin was proportioned to the rate of synthesis. Variants which had spontaneously lost the ability to produce heavy chains continued to synthesize light chains at approximately the same rate as their parent cell line.

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“…A method for the autoradiographic localization of intracellular antibody has been described by Berenbaum (1,2,3). It involves the application of radioactively labeled protein or polysaccharide anti gen to slides prepared from imprints of lymphoid organs from animals previously immunized with the corresponding non-labeled antigen.…”

mentioning

confidence: 99%