The Antibacterial Mechanism of Terpinen-4-ol Against Streptococcus agalactiae

Zhang, Yuetian; Feng, Ruizhang; Li, Lixia; Zhou, Xun; Li, Zhengwen; Jia, Renyong; Song, Xu; Zou, Yuanfeng; Yin, Lizi; He, Chao; Liang, Xiaoxia; Zhou, Wanhai; Qin, Wei; Du, Yonghua; Ke, Yan; Wu, Zili

doi:10.1007/s00284-018-1512-2

Cited by 58 publications

(43 citation statements)

References 26 publications

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“…The starting voltage was 60 V and the voltage of the sample changed to 120 V after entering the separating gel. After completing the electrophoresis, the gel was removed and then stained with Coomassie Brilliant Blue R-250, and the separated protein bands were obtained after decolorization [39].…”

Section: Leakage Of Proteinsmentioning

confidence: 99%

Antimicrobial Susceptibility and Antibacterial Mechanism of Limonene against Listeria monocytogenes

2019

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Limonene is a monoterpenoid compound, which is founded in a lot of plants' essential oils with good antibacterial activity against food-borne pathogens, but it has an ambiguous antimicrobial susceptibility and mechanism against Listeria monocytogenes (L. monocytogenes). In this study, the antimicrobial susceptibility of Limonene to L. monocytogenes was studied, and some new sights regarding its antibacterial mechanism were further explored. Scanning electron MICroscopy (SEM) verified that limonene caused the destruction of the cell integrity and wall structure of L. monocytogenes. The increase in conductivity and the leakage of intracellular biomacromolecules (nucleic acids and proteins) confirmed that limonene had an obvious effect on cell membrane permeability. The results of Propidium Iodide (PI) fluorescence staining were consistent with the results of the conductivity measurements. This indicated that limonene treatment caused damage to the L. monocytogenes cell membrane. Furthermore, the decrease in ATP content, ATPase (Na + K + -ATPase, Ca 2+ -ATPase) activity and respiratory chain complex activity indicated that limonene could hinder ATP synthesis by inhibiting the activity of the respiratory complex and ATPase. Finally, differential expression of proteins in the respiratory chain confirmed that limonene affected respiration and energy metabolism by inhibiting the function of the respiratory chain complex.Molecules 2020, 25, 33 2 of 15 terpenes in nature and is widely found in the volatile oils of various plants (black pepper, lemon and orange, etc.) [9]. Limonene has broad application prospects in antibacterial and food preservation due to its broad-spectrum bactericidal activity, safety, and low toxicity [10]. Moreover, D-limonene can significantly inhibit gram-negative and gram-positive bacteria as well as fungal activity [11]. In addition, many researchers have confirmed that D-limonene can effectively inhibit the growth of spoilage bacteria, such as Aspergillus niger, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli [12,13]. Limonene, which is the main ingredient of lemon essential oil, was found to have antimicrobial activities against L. monocytogenes in minced beef meat [14]. However, few studies have investigated the antibacterial mechanism of limonene.The aims of this study were to determine the antibacterial susceptibility and its antibacterial mechanism of limonene against L. monocytogenes. The minimum inhibitory concentration (MIC) was used to evaluate the anti-L. monocytogenes susceptibility of limonene. The growth curves of the bacterial were determined for evaluating the effect of limonene on the growth and reproduction of L. monocytogenes. The mechanism of action of limonene was explored by analysing its influence on the cell morphology, membrane permeability and changes in the protein, nucleic acid, ATP, ATPase (Na + K + -ATPase, Ca 2+ -ATPase), respiratory chain complex I~V, and differential protein expression of the respiratory chain complex of L. monocytogenes. Resu...

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Section: Leakage Of Proteinsmentioning

confidence: 99%

Antimicrobial Susceptibility and Antibacterial Mechanism of Limonene against Listeria monocytogenes

2019

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“…A 20-µL volume of the bacterial suspension was then used to create slides, which were observed with a fluorescence microscope (LeicaDM1000, Leica). 46,47 UV Spectroscopic Test of the Interaction Between Luteolin and DNA Luteolin (final concentration, 1/2 MIC) was fully mixed with pBR322 DNA (1 µg/mL, TAKARA, Dalian, China) and incubated at 37 ºC for 30 min. The absorption spectrum, which ranged from 300 to 420 nm, was determined by an ultraviolet spectrophotometer (U-3900/3900H, Hitachi, Japan).…”

Section: Effect Of Luteolin On Nucleic Acidmentioning

confidence: 99%

<p>The Antibacterial Activity and Mechanism of Action of Luteolin Against <em>Trueperella pyogenes</em></p>

Guo¹,

Liu²,

Zhang³

et al. 2020

IDR

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Purpose: This research aimed to investigate the antibacterial activity and potential mechanism of luteolin against T. pyogenes. Materials and Methods: The broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) of luteolin against various T. pyogenes strains. The potential mechanism of action of luteolin was elucidated through testing and analysing the luteolin-induced alterations of T. pyogenes in several aspects, including cell wall, cell membrane, protein expression, nucleic acid content, topoisomerase activity and energy metabolism. Results: The MIC values of luteolin against various T. pyogenes isolates and ATCC19411 were 78 µg/mL. The increased cell membrane permeability, destruction of cell wall integrity and TEM images after exposure to luteolin showed that the cell wall and membrane were damaged. The content of total protein and nucleic acid in T. pyogenes decreased significantly after treatment with luteolin (1/2 MIC) for 12, 24, and 36 h. Moreover, a hypochromic effect was observed in the absorption spectrum of luteolin when deoxyribonucleic acid (DNA) was added. In addition, after treatment with luteolin, a decrease in nicked or relaxed DNA content, which was catalysed by T. pyogenes-isolated DNA topoisomerase, was observed. In addition, the adenosine triphosphate (ATP) content in cells and the activity of succinate dehydrogenase (SDH) both decreased when T. pyogenes was exposed to different concentrations (1/4 MIC, 1/2 MIC, 1 MIC, 2 MIC) of luteolin for 1 h. Conclusion: Luteolin showed distinct antibacterial activity against T. pyogenes by multiple actions, which mainly include destroying the integrity of the cell wall and cell membrane, influencing the expression of proteins, inhibiting nucleic acid synthesis, and interfering with energy metabolism.

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“…The major compound, α-terpineol, from rhizomes of A. scabra and A. murdochii in this study has been reported by Li et al (2014) to exhibit strong antibacterial activity against Salmonella enteritidis and Staphylococcus aureus with MIC values of 1.56 and 3.13 µg/mL, respectively. Another study by Zhang et al (2018) showed that terpinen-4-ol exhibit anti-bacterial activity with MIC value of 98 µg/mL against Staphylococcus agalactiae. Terpinen-4-ol was present in leaf and rhizome oils of A. scabra and A. murdochii but in higher quantities in the latter.…”

Section: Anti-microbial Activitymentioning

confidence: 99%

Essential Oil Constituents of Alpinia scabra and Alpinia murdochii, Two Wild Highland Species from Peninsular Malaysia and Their Anti-Microbial Activity

Syamsir

Tohar

Ibrahim

et al. 2020

JSM

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This study investigates the chemical constituents of essential oils and their anti-microbial activity of Alpinia scabra and Alpinia murdochii, two wild Zingiberaceae species. The essential oils were obtained by hydrodistillation and the chemical components of the oils were determined by GC-FID (retention indices, RI) and GC/MS analysis. The major components of Alpinia scabra rhizome oil are γ-selinene (33.45%), α-selinene (3.64%) and α-terpineol (3.55 %) while the major components of the leaves are β-pinene (63.37%), α-pinene (6.58 %) and borneol (3.20 %). The major compounds of Alpinia murdochii rhizome oil are γ-selinene (15.51 %), (E,E)-farnesyl acetate (6.56 %), terpinen 4-ol (5.58 %) and α-terpineol (5.04 %). The monoterpenes; β-pinene (23.83 %), sabinene (23.76 %) and terpinene-4-ol (10.49 %) were the major components in the leaf oil of A. murdochii. The lowest MIC values were recorded for the rhizome essential oils of both Alpinia species against all Staphylococcus aureus strains (coded as MSSA, MRSA, Sa7, VISA24, VRSA156) with MIC values ranging from 0.04 mg/mL to 2.50 mg/mL. The rhizome oils of both species also showed a broad spectrum of anti-microbial activity as compared to the leaf oils.

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The Antibacterial Mechanism of Terpinen-4-ol Against Streptococcus agalactiae

Cited by 58 publications

References 26 publications

Antimicrobial Susceptibility and Antibacterial Mechanism of Limonene against Listeria monocytogenes

Antimicrobial Susceptibility and Antibacterial Mechanism of Limonene against Listeria monocytogenes

<p>The Antibacterial Activity and Mechanism of Action of Luteolin Against <em>Trueperella pyogenes</em></p>

Essential Oil Constituents of Alpinia scabra and Alpinia murdochii, Two Wild Highland Species from Peninsular Malaysia and Their Anti-Microbial Activity

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