The Antibacterial Activity of Crude Extracts of Secondary Metabolites from Bacterial Endophytes Associated with Dicoma anomala

Makuwa, Sephokoane Cindy; Serepa-Dlamini, Mahloro Hope

doi:10.1155/2021/8812043

Cited by 18 publications

(13 citation statements)

References 39 publications

(29 reference statements)

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“…A study by Akpor et al (2021), determined the antibacterial potential of metabolites produced by B. proteolyticus, B. thuringiensis, B. cereus, and B. subtilis, and all the extracts showed antimicrobial activity against test pathogens at an MIC of 200 mg/ml. In a similar study by Makuwa and Serepa-Dlamini (2021), bioactive metabolites of Bacillus species isolated from a medicinal plant, Dicoma anomala were found to be effective against selected pathogens such as E. coli, K. oxytoca, and S. aureus with MIC values ranging from 0.625 to 10 mg/ml. According to Borriss et al (2019), Bacillus species produce antibacterial agents such as surfactin and bacteriocins which may be responsible for their antibacterial activities (Huo et al, 2019).…”

Section: Discussionmentioning

confidence: 88%

The Antibacterial, Antitumor Activities, and Bioactive Constituents’ Identification of Alectra sessiliflora Bacterial Endophytes

2022

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Due to increased antimicrobial resistance against current drugs, new alternatives are sought. Endophytic bacteria associated with medicinal plants are recognized as valuable sources of novel secondary metabolites possessing antimicrobial, antitumor, insecticidal, and antiviral activities. In this study, five bacterial endophytes were isolated and identified from the medicinal plant, Alectra sessiliflora, and their antibacterial and antitumor activities were investigated. In addition, the crude extracts of the endophytes were analyzed using gas chromatography (GC) coupled with time-of-flight mass spectrometry (TOF-MS). The identified bacterial endophytes belong to three genera viz Lysinibacillus, Peribacillus, and Bacillus, with the latter as the dominant genus with three species. Ethyl acetate extracts from the endophytes were used for antimicrobial activity against eleven pathogenic strains through minimum inhibitory concentration (MIC). The antitumor activity against the Hela cervical, Hek 293 kidney, and A549 lung carcinoma cells was determined by the MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2H-tetrazolium] assay. Lysinibacillus sp. strain AS_1 exhibited broad antibacterial activity against the pathogenic strains with MIC values ranging from 4 to 8 mg/ml, while Bacillus sp. strain AS_3 displayed MIC of 0.25 mg/ml. Crude extracts of Lysinibacillus sp. strain AS_1, Peribacillus sp. strain AS_2, and Bacillus sp. strain AS_3 showed growth inhibition of more than 90% against all the cancer cell lines at a concentration of 1,000 μg/ml. Untargeted secondary metabolite profiling of the crude extracts revealed the presence of compounds with reported biological activity, such as antimicrobial, antioxidant, anti-inflammatory, antitumor, and antidiabetic properties. This study reported for the first time, bacterial endophytes associated with A. sessiliflora with antibacterial and antitumor activities.

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Section: Discussionmentioning

confidence: 88%

The Antibacterial, Antitumor Activities, and Bioactive Constituents’ Identification of Alectra sessiliflora Bacterial Endophytes

2022

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“…After seven days, the depletion of nutrients in the medium force bacteria to get into the stationary phase and release secondary metabolites. In several studies, secondary metabolites were obtained after seven days’ incubation period of bacteria [5, 48, 53]. Among the tested crude extracts, K. aerogenes ’s extract displayed strong antibacterial activities against both Gram-positive and Gram-negative bacteria.…”

Section: Discussionmentioning

confidence: 99%

Anticancer and antibacterial activities of secondary bioactive compounds from endosymbiotic bacteria of aphid and their surrounding organisms (Predator and protector)

Alsufyani

Al-Otaibi

Alotaibi

et al. 2022

Preprint

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Secondary metabolites of bacterial origin are a valuable source of diverse molecules with antibacterial and anticancer activities. In the current study, 10 endosymbiotic bacteria were isolated from aphids, aphid predators and ants. Bacterial strains were identified based on the 16S rRNA gene. Crude extracts were prepared from each isolated bacteria and tested for their antibacterial activities using the disk diffusion method. The extracts of three bacterial species;Planococcussp,Klebsiella aerogenes, Enterococcus aviumfromAphis punicae, Chrysoperia carneaandTapinoma magnum, respectively were found to have strong antibacterial activities against one or more of the five pathogenic bacteria tested. The inhibition zones ranged from 10.00± 0.13 to 20.00± 1.11mm with minimum inhibitory concentration (MIC) values ranged from 0.156 mg/mL to 1.25 mg/mL. The notable antibacterial activity was for the crude extract ofK. aerogenesagainstKlebsiella pneumoniaandEscherichia coliat MIC value of 0.156 mg/mL. The cytotoxic activity of the crude extracts varied according to the tested cell line. The most cytotoxic effect was for the extracts ofK. aerogenesandE. aviumat a concentration of 0.16 mg/mL against lung carcinoma epithelial cells (A549) with cell reduction of 79.4% and 67.2%, respectively. Crude extracts ofK. aerogenesandPantoea agglomeransat a concentration of 0.16 mg/mL showed 69.4% and 67.8% cell reduction against human colon cancer (Hct116), respectively. Gas chromatography–mass spectrometry (GC-MS) analysis of three crude extracts revealed the presence of several bioactive secondary metabolites that have been reported previously to possess antibacterial and anticancer properties. To the best of our knowledge, this is the first study to report the biological activities of endosymbiotic bacteria from aphids, aphid predators and ants. The promising data presented in this study opens a new avenue for alternative drugs to overcome the continuous emergence of multidrug-resistant bacteria and to find alternative drugs for traditional cancer therapies.

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“…The antibacterial properties of the secondary metabolites of plants have been in the foreground of research in the last two decades [ 189 , 190 , 191 , 192 , 193 , 194 , 195 ]. Such research is even more important considering the emergence of numerous resistant [ 196 , 197 ] and multi-drug resistant (MRD) bacteria [ 198 ].…”

Section: Kaempferol As An Antibacterial Agentmentioning

confidence: 99%

Kaempferol: Antimicrobial Properties, Sources, Clinical, and Traditional Applications

Periferakis

Bădărău

et al. 2022

IJMS

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Flavonoids are a category of plant-derived compounds which exhibit a large number of health-related effects. One of the most well-known and studied flavonoids is kaempferol, which can be found in a wide variety of herbs and plant families. Apart from their anticarcinogenic and anti-inflammatory effects, kaempferol and its associated compounds also exhibit antibacterial, antifungal, and antiprotozoal activities. The development of drugs and treatment schemes based on these compounds is becoming increasingly important in the face of emerging resistance of numerous pathogens as well as complex molecular interactions between various drug therapies. In addition, many of the kaempferol-containing plants are used in traditional systems all over the world for centuries to treat numerous conditions. Due to its variety of sources and associated compounds, some molecular mechanisms of kaempferol antimicrobial activity are well known while others are still under analysis. This paper thoroughly documents the vegetal and food sources of kaempferol as well as the most recent and significant studies regarding its antimicrobial applications.

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The Antibacterial Activity of Crude Extracts of Secondary Metabolites from Bacterial Endophytes Associated with Dicoma anomala

Cited by 18 publications

References 39 publications

The Antibacterial, Antitumor Activities, and Bioactive Constituents’ Identification of Alectra sessiliflora Bacterial Endophytes

The Antibacterial, Antitumor Activities, and Bioactive Constituents’ Identification of Alectra sessiliflora Bacterial Endophytes

Anticancer and antibacterial activities of secondary bioactive compounds from endosymbiotic bacteria of aphid and their surrounding organisms (Predator and protector)

Kaempferol: Antimicrobial Properties, Sources, Clinical, and Traditional Applications

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