The Anti-inflammatory Effects of Water Extract fromCordyceps militarisin Murine Macrophage

Abstract

The aim of this study was to determine the in vitro anti-inflammatory effect of hot water extract from Cordyceps militaris fruiting bodies (CMWE) on lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) release in RAW 264.7 cells. The treatment of macrophages with various concentrations of hot CMWE significantly reduced LPS-induced production as well as NO, TNF-α and IL-6 secretion in a concentration-dependent manner. These results suggest that CMWE have potent inhibitory effects on the production of these inflammatory mediators.

Cordyceps militarisalleviates non-alcoholic fatty liver disease in ob/ob mice

“…C. militaris also showed a tendency to decrease serum MCP-1, although the difference was not statistically significant. These findings are in agreement with a previous report that C. militaris reduced TNF-α and IL-6 secretion by LPS-treated murine macrophages [18]. TNF-α mediates insulin resistance by impairing insulin signal transduction [38].…”

“…C. militaris extract has alleviated oxidative injury in HepG2 cells induced by tert-butyl hydroperoxide (t-BHP) by reducing reactive oxygen species (ROS) generation and thiobarbituric acid reactive substances (TBARS) formation. In addition, C. militaris has demonstrated anti-inflammatory activity in vitro [18]. A hot water extract of C. militaris reduced production of nitric oxide (NO) and secretion of TNF-α and IL-6 induced by lipopolysaccharide (LPS) in macrophages.…”

An insight into anti-inflammatory effects of fungal beta-glucans

“…Most of the research has focused on the secretion of pro-inflammatory cytokines or mRNA expression of inflammatory mediators such as IL-1, IL-6, IL-8, IL-12, IL-18, and interferon-g (IFN-g). Jo et al (2010) determined the in vitro anti-inflammatory effect of hot water extract (mainly b-glucan) from fruiting bodies of Cordyceps militaris on lipopolysaccharide (LPS)-stimulated NO production, TNF-a and IL-6 release in RAW 264.7 cells. The treatment of macrophages with various concentrations of hot water extract from C. militaris significantly reduced LPS-induced NO production as well as secretion of TNF-a and IL-6 in a concentration-dependent manner.…”

β-Glucans and Probiotics

“…Anti-inflammatory activities of β-glucans from fungi were emphasized by other studies that proved their ability to significantly reduce NO production and secretion of TNF-α and IL-6 in LPS-stimulated macrophages (Jo et al, 2010). β-Glucans, from both fungi and yeast, were demonstrated to hinder the secretion of TNF-α, IL-1α, IL-1 and NO, decreasing the relative gene expression possibly via suppression of MAP kinases JNK1/2 and ERK1/2 (Xu et al, 2012a(Xu et al, , 2012b.…”