The Anti-Inflammatory and Antinociceptive Properties of the Chloroform Fraction FromPhyllanthus niruriPlant Is Mediated via the Peripheral Nervous System

Abstract

Phyllanthus niruri (Euphorbiaceae) is used folklorically for the treatment of diabetes, malaria, fever, diarrhea, liver disease, and urolithiasis. As an initial step toward isolating compounds effective against inflammation and pain, this study is aimed at providing scientific evidence for the anti-inflammatory, antinociceptive, and antipyretic properties of the chloroform soluble fraction (PNF1) of Phyllanthus niruri methanol extract in rats and mice. Three doses of PNF1 [25, 50, 100 mg/kg body weight (bw)] were used. Screening was done using acetic-acid-induced writhing, egg-albumin-induced pedal inflammation, Randall-Selitto test, hot-plate test, and yeast-induced pyresis as experimental models. Results show that PNF1 significantly (p < .01) inhibited writhing response induced by acetic acid at all doses used by 56.2%-66.7% and caused significant (p < .05, p < .01) reduction of yeast-induced pyrexia (21.6%-40.9%). Significant (p < .01) reduction of egg albumin-induced inflammation was observed only at a dose of 100 mg PNF1/kg bw, which was comparable with the effect produced by aspirin (100 mg/kg bw). At 50 and 100 mg/kg bw, PNF1 significantly (p < .05, p < .01) increased pain threshold of inflamed tissue in the Randall-Selitto test but did not increase response to thermally induced pain in the hot-plate test. It is concluded that PNF1 possesses antipyretic, anti-inflammatory, and antinociceptive effects that are peripherally mediated. This justifies its use in traditional medicine and its potential as a candidate for further development.

Medicinal plants in the treatment ofHelicobacter pyloriinfections

“…Recently, hepatoprotective (Ho et al, 2012), antipyretic, anti-inflammatory, antinociceptive (Obidike et al, 2010), antiplasmodial (Mustofa et al, 2007), and antitumor activities by enhancing antioxidant defense (Sharma et al, 2011) have been reported for Phyllanthus niruri L. (Chancapiedra). In a screening test for the detection of plants with anti-H. pylori activity, P. niruri showed anti-H. pylori effects against metronidazole-resistant H. pylori strains (Uyub et al, 2010).…”

The use of Phyllanthus niruri L. as an immunomodulator for the treatment of infectious diseases in clinical settings

“…The anti-nociceptive property of P. niruri [81] altogether with the antibacterial activity of P. niruri-N. sativa combination might contribute to the effectiveness of the treatment with the combined extracts [82]. An in vitro study demonstrated that various pathogenic bacteria, including Streptococcus pneumoniae, one of the most common causes of bacterial tonsillopharyingitis, were sensitive to the combined P. niruri-N. sativa extract.…”

Efficacy and Safety of Phyllanthus Niruri in Non-alcoholic Steatohepatitis Treatment: Pilot Study from Malaysia

“…[5][6][7][8] Phyllanthus niruri, a herb extensively used in traditional Malay, Chinese and Ayurvedic medicine, is a potential therapeutic agent for a wide range of liver diseases, as laboratory studies have confirmed its antiviral, anti-inflammatory, antioxidant and hepatoprotective properties. [9][10][11][12][13] However, it has never been tested in patients, particularly those with NASH. Hence, this pilot study was designed to confirm the efficacy and safety of a commercially available product, HEPAR-P ® (Nova Laboratories), which was standardized to contain 250 mg of Phyllanthus niruri extract EPN 797 (4% of corilagin and 18% of phyllanthus flavonoids) per capsule.…”