The anti-giardial effectiveness of fungal and commercial chitosan against Giardia intestinalis cysts in vitro

Abstract

Chitosan with poly-N-acetylglucosamine sequences is a deacetylated derivative of chitin that can be found in the exoskeletons of crabs, shrimp and lobsters, the cuticles of insects and the cell walls of fungi. The aim of the present study was to compare the effects of fungal chitosan (FC) prepared from the cell walls of Penicillium viridicatum and Penicillium aurantiogriseum with commercially available chitosan (CC) against Giardia intestinalis cysts in vitro. The giardia cysts were isolated using a sucrose method. Four concentrations (50, 100, 200 and 400 lg/ml) of each type of prepared chitosan were applied for 10, 30, 60 and 180 min. The viability of the cysts was checked via 0.1 % eosin staining. Our results indicate that P. viridicatum (with a 47.5 % DD) and P. aurantiogriseum (with a 47.3 % DD) at different concentrations after 180 min precipitated, respectively, 56, 69, 81 and 100 %, and 63, 75, 86 and 100 % mortality rates. CC (with a 54 % DD) showed 79, 84, 93 and 100 % mortality rates. In conclusion, both FC and CC at 400 lg/ml concentrations after 180 min of exposure showed the most potent effect against G. intestinalis cysts. Accordingly, chitosan could be suggested as a new natural nanoform agent for future research in the safe and effective treatment of Giardia infections.

Anti-<em>Toxoplasma</em> activity of various molecular weights and concentrations of chitosan nanoparticles on tachyzoites of RH strain

“…34 Similar studies have shown that 1.250 µg/mL of CS after 360 min and 400 µg/mL of the component after 180 min of exposure completely inhibit the viability of Trichomonas gallinae trophozoites and Giardia lamblia cysts, respectively. 35,36 To improve the therapeutic efficiency, NPs have been used widely as vehicles to deliver drugs or vaccines in previous studies. 7,9,10,14 However, a few studies have been carried tool for the treatment of Leishmania infantum, Plasmodium falciparum and Cryptosporidium parvum.…”

Antiparasitic Activity of Silver and Copper Oxide Nanoparticles against Entamoeba Histolytica and Cryptosporidium Parvum Cysts

“…Cysts without absorbed dye were recorded as potentially viable; otherwise recorded as dead one. The tests were repeated in triplicate (Yarahmadi et al, 2014).…”

Evaluation of the diagnostic performance of Nano gold beads-based ELISA for Detection of circulating Toxoplasma surface antigen grade III (SAG3) in Serum Samples of Infected cases.

“…Different materials were used for nanoparticles synthesis and utilized in medical science and for treatment of some infections like silver, chitosan, gold, etc. [21] . The application of gold's nanoparticles nowadays turns into a popular option in industrial contexts, scientific and medical science.…”