Antiparasitic Activity of Silver and Copper Oxide Nanoparticles against Entamoeba Histolytica and Cryptosporidium Parvum Cysts

Saad, Halim A; Soliman, Mohamed H.; Azzam, Ahmed M.; Mostafa, B

doi:10.12816/0017920

Cited by 47 publications

(39 citation statements)

References 32 publications

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“…Taken together, our data provide evidence for the anti-bradyzoite potential of NPs but demonstrate that the anti-T. gondii potential of NPs may not necessarily preclude bradyzoite formation. Our findings support the anti-parasitic action of NPs and are consistent with other reports that have demonstrated the anti-protozoal (Ahmad et al 2015, Rahul et al 2015, Saad et al 2015, Yah and Simate 2015, Saini et al 2016, including anti-T. gondii (Leyke et al 2012, Adeyemi et al 2017, 2018a, potential of NPs.…”

Section: Discussionsupporting

confidence: 93%

Nanoparticles show potential to retard bradyzoites in vitro formation of Toxoplasma gondii

Adeyemi¹,

Murata²,

Sugi³

et al. 2019

FOLIA PARASIT

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Toxoplasmosis is a common parasitic disease caused by Toxoplasma gondii (Nicolle et Manceaux, 1908), an obligate parasite capable of infecting a range of cell types in almost all warm-blooded animals. Upon infecting an intermediate host, the parasites differentiate into tachyzoites which rapidly infect host tissues. Usually, the invading parasites are cleared by the immune system and administered drugs, but some tachyzoites differentiate into bradyzoites forming tissue cysts. These tissue cysts could serve as a source for re-infection and exacerbations. Currently, treatment for toxoplasmosis is limited and, moreover, there are no drugs for treating the cystic stage thus rendering toxoplasmosis a global burden. Recently, we demonstrated that inorganic nanoparticles showed promising activity against the tachyzoite stage T. gondii. In the present study, we evaluated nanoparticles for effect on bradyzoite formation in vitro. Data revealed that the nanoparticles limited bradyzoite burden in vitro. Further, the nanoparticles decreased the bradyzoite-specific BAG-1 promoter activity relative to the untreated control under a bradyzoite-inducing culture condition, even though this reduction in BAG-1 promoter activity waned with increasing concentrations of nanoparticles. In contrast, a parallel experiment under normal cell culture conditions showed that the nanoparticle treatment mildly increased the BAG-1 promoter activity relative to the untreated control. Taken together, the findings are evidence that nanoparticles not only possess anti-tachyzoite potential but they also have anti-bradyzoite potential in vitro.

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Section: Discussionsupporting

confidence: 93%

Nanoparticles show potential to retard bradyzoites in vitro formation of Toxoplasma gondii

Adeyemi¹,

Murata²,

Sugi³

et al. 2019

FOLIA PARASIT

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“…In addition, the poor recovery of parasites as well as the marginal parasite infectivity after treatment with NPs supports the further exploration of NPs as anti-infective agents. Together, these data show that NPs have favorable anti-T. gondii potential and thus support several earlier reports that demonstrated the anti-Cryptosporidium parvum, 28 antileishmanial, 16 antifilarial, 30 antitrypanosomal, and antimalarial activities 17 of AgNPs as well as the antiparasitic activity of AuNPs. 17,30 However, this is the first report to show that AuNPs, AgNPs, and PtNPs can cause the death of T. gondii both within the host cell and extracellularly.…”

Section: Discussionsupporting

confidence: 91%

“…We obtained antiparasite EC 50 values of ,7, ,1, and ,100 µg/mL for AuNPs, AgNPs, and PtNPs, respectively. Although the published reports of the antimicrobial and/or antiparasitic properties of PtNPs are limited, the EC 50 values obtained in our study are well within the range of those reported elsewhere 16,17,[28][29][30] for the antimicrobial and/or antiparasitic action exhibited by AuNPs and AgNPs. Interestingly, a few of the studies 17,29,30 reporting the antiparasitic activities of NPs revealed that under the same conditions, AgNPs had a lower EC 50 value relative to that of AuNPs.…”

Section: Discussionsupporting

confidence: 89%

Inorganic nanoparticles kill <em>Toxoplasma gondii</em> via changes in redox status and mitochondrial membrane potential

Adeyemi¹,

Murata²,

Sugi³

et al. 2017

IJN

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This study evaluated the anti-Toxoplasma gondii potential of gold, silver, and platinum nanoparticles (NPs). Inorganic NPs (0.01–1,000 µg/mL) were screened for antiparasitic activity. The NPs caused >90% inhibition of T. gondii growth with EC50 values of ≤7, ≤1, and ≤100 µg/mL for gold, silver, and platinum NPs, respectively. The NPs showed no host cell cytotoxicity at the effective anti-T. gondii concentrations; the estimated selectivity index revealed a ≥20-fold activity toward the parasite versus the host cell. The anti-T. gondii activity of the NPs, which may be linked to redox signaling, affected the parasite mitochondrial membrane potential and parasite invasion, replication, recovery, and infectivity potential. Our results demonstrated the antiparasitic potential of NPs. The findings support the further exploration of NPs as a possible source of alternative and effective anti-T. gondii agents.

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“…At present, synthesized nanoparticles have been proposed as a new generation of anti-microbial, anti-viral and antifungal agents [13,14], and nanoparticles activity against different protozoans, such as Giardia intestinalis, Entamoeba histolytica, Cryptosporidium parvum and Leishmania spp. has been already confirmed [15,16,17]. It has been demonstrated in previous studies in our laboratory that silver and gold nanoparticles were well absorbed and showed activity against Acanthamoeba strains belonging to the T4 genotype [18].…”

Section: Introductionsupporting

confidence: 67%

Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains

Padzik¹,

Hendiger²,

Żochowska³

et al. 2019

Ann Agric Environ Med.

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Introduction. Various Acanthamoeba species are free-living organisms widely distributed in the human environment. Amphizoic amoebae as facultative parasites may cause vision-threatening eye disease-Acanthamoeba keratitis, mostly among contact lens wearers. As the number of cases is increasing, and applied therapy often unsuccessful, proper hygienic measures and effective contact lenses disinfection are crucial for the prevention of this disease. Available contact lens solutions are not fully effective against amphizoic amoebae; there is a need to enhance their disinfecting activity to prevent amoebic infections. The use of developing nanotechnology methods already applied with success in the prevention, diagnostic and therapy of other infectious diseases might be helpful regarding amoebic keratitis. This study assesses the in vitro effect of selected contact lens solutions conjugated with nanoparticles against Acanthamoeba trophozoites. Materials and method. Three selected contact lens solutions conjugated with silver and gold nanoparticles in concentration of 0.25-2.5 ppm were used in vitro against the axenically cultured ATCC 30010 type Acanthamoeba castellanii strain. The anti-amoebic efficacy was examined based on the oxido-reduction of AlamarBlue. The cytotoxicity tests based on the measurement of lactate dehydrogenase (LDH) activity were performed using a fibroblast HS-5 cell line. Results. Enhancement of the anti-amoebic activity of contact lens solutions conjugated with selected nanoparticles expressed in the dose dependent amoebic growth inhibition with a low cytotoxicity profile was observed. Conclusions. Results of the study showed that conjugation of selected contact lens solutions with silver nanoparticles might be a promising approach to prevent Acanthamoeba keratitis among contact lens users.

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Antiparasitic Activity of Silver and Copper Oxide Nanoparticles against Entamoeba Histolytica and Cryptosporidium Parvum Cysts

Cited by 47 publications

References 32 publications

Nanoparticles show potential to retard bradyzoites in vitro formation of Toxoplasma gondii

Nanoparticles show potential to retard bradyzoites in vitro formation of Toxoplasma gondii

Inorganic nanoparticles kill <em>Toxoplasma gondii</em> via changes in redox status and mitochondrial membrane potential

Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains

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