The antagonistic mechanism of rhizosphere microbes and endophytes on the interaction between banana and Fusarium oxysporum f. sp. cubense

Mon, Yin Yin; Bidabadi, Siamak Shirani; Oo, Kyaw Swar; Zheng, Si-Jun

doi:10.1016/j.pmpp.2021.101733

Cited by 13 publications

(6 citation statements)

References 93 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…to soil has been shown to reduce the severity of FWB [57,[96][97][98][99], with concomitant increases in the relative abundances of Gemmatimonas, Sphingomonas, Rhizobium and/or Pseudolabrys populations [100]. Lastly, many studies have demonstrated suppression of Foc in culture by close relatives of the common core isolated from banana [101], including members of the Bacillus [102,103], Pseudomonas [59,104,105] Rhizobium [104], and Streptomycetes [106].…”

Section: Associations Of Core Taxa With Host Fitnessmentioning

confidence: 99%

The core bacterial microbiome of banana (Musa spp.)

Birt

Pattison

Skarshewski

et al. 2022

Environmental Microbiome

View full text Add to dashboard Cite

Background Bananas (Musa spp.) are a globally significant crop and are severely afflicted by diseases for which there are no effective chemical controls. Banana microbiomes may provide novel solutions to these constraints but are difficult to manage due to their high diversity and variability between locations. Hence ‘common core’ taxa, which are a subset of the microbiome that frequent all, or most, individuals of a host species, represent logical targets for the development of microbiome management approaches. Here, we first performed a pot experiment to characterise the effects of two factors that are likely to differ between farms (viz. edaphic conditions and host genotype) on bacterial diversity in bulk soil and seven plant compartments. From this experiment, we created shortlisted core ‘candidates’ that were then refined using a survey of 52 field-grown Musa spp. We confirmed the importance of the core through network analysis and by comparing the sequences of our core taxa with those reported in 22 previous studies. Results Diversity was found to differ between plant compartments and soils, but not genotypes. Therefore, we identified populations that were frequent across most plants irrespective of the soil in which they were grown. This led to the selection of 36 ‘common core’ bacteria, that represented 65–95% of the dominant taxa in field-grown plants and were identified as highly interconnected ‘hubs’ using network analysis – a characteristic shown to be indicative of microbes that influence host fitness in studies of other plants. Lastly, we demonstrated that the core taxa are closely related to banana-associated bacteria observed on five other continents. Conclusions Our study provides a robust list of common core bacterial taxa for Musa spp. Further research may now focus on how changes in the frequencies and activities of these most persistent taxa influence host fitness. Notably, for several of our core taxa, highly similar populations have already been isolated in previous studies and may be amenable to such experimentation. This contribution should help to accelerate the development of effective Musa spp. microbiome management practices.

show abstract

Section: Associations Of Core Taxa With Host Fitnessmentioning

confidence: 99%

The core bacterial microbiome of banana (Musa spp.)

Birt

Pattison

Skarshewski

et al. 2022

Environmental Microbiome

View full text Add to dashboard Cite

show abstract

“…Antagonistic microorganisms have been efficiently used as biological control agents to suppress numerous plant diseases (Mon et al, 2021). In this study, we isolated a B. velezensis JIN4 from kiwifruit inter-root soil and demonstrated its biocontrol potential.…”

Section: Discussionmentioning

confidence: 94%

Isolation, identification, and genome of a Bacillus velezensis JIN4 exhibiting biocontrol activity against kiwifruit bacterial canker

Zhao,

Zhai,

et al. 2023

Preprint

View full text Add to dashboard Cite

Kiwifruit bacterial canker is a devastating disease in production. This study aimed to screen antagonistic bacteria that might be used to control this disease. Strain JIN4 showed significant antifungal activity against Pseudomonas syringae pv. Actinidiae (Psa) and seven other phytopathogenic bacteria isolated from kiwifruit branches. Based on morphological, physiological, and biochemical characteristics, 16S rDNA sequence analysis, and gyrA sequence analysis, JIN4 was identified as Bacillus velezensis. In field prevention experiments, the control efficiency of strain JIN4 reached 60.22%. Our results indicate that B. velezensis JIN4 has broad-spectrum antifungal resistance, and its genome contains many genes encoding enzymes involved in the synthesis of antimicrobial metabolites. In addition, JIN4 has three antibiotic-resistance genes and a cluster of nine genes used in the synthesis of secondary metabolites with antimicrobial activity. In conclusion, B. velezensis JIN4 can be used as a potential biocontrol agent against kiwifruit bacterial canker, but further work is required to improve its efficacy.

show abstract

“…Such is the case of the Fusarium Wilt of Banana (FWB), a widely distributed disease caused by several Fusarium species, including F. oxysporum f. sp. cubense (Foc) (Mon et al, 2021;Ploetz, 2015). An eradication method for FWB is not currently known (Ismaila et al, 2022); however, biological control is a very promising approach to protect banana crops against Foc (Abram et al, 2021;Dimkić et al, 2022;Köhl et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Impaired bioenergetic response mediates the antifungal effect of lipopeptides from Bacillus tequilensis EA-CB0015 and cinnamon extract against Fusarium oxysporum f. sp. cubense

Ramírez-Mejía

Villegas-Escobar

Gómez

2023

Preprint

View full text Add to dashboard Cite

Lipopeptides (LPs) from B. tequilensis EA-CB0015 have antifungal activity against Fusarium species. Specifically, against F. oxysporum f. sp. cubense (Foc), the cause of the Fusarium wilt of banana. In addition, cinnamon (CN) extract is also known for its inhibitory activity against the Fusarium genus. The main goal of this research was to determine if the effect of LPs, CN extract or their combination against Foc is related to an impairment of mitochondrial function. Our results show that biomass decreased by 74% (p < 0.0001) and 84% (p < 0.0001) when cultures were treated with 128 ppm LPs and 152.5 ppm CN extract, respectively. In parallel, we found a pronounced impairment of the bioenergetic response. That is, the routine oxygen consumption rate diminished by 55% (p = 0.0148) and 38% (p < 0.0001), respectively. Moreover, the ATP-linked respiratory rate decreased by 63% (p = 0.0461) and 44% (p = 0.0005), while the FCCP-simulated respiratory rate by 63% (p = 0.0255) and 45% (p = 0.0002). Therefore, our data suggest that the altered bioenergetic response observed in cultures of Foc treated with LPs or CN is mainly caused by an impairment of the activity of the ETC components. On the other hand, biomass production was reduced by 80% (p < 0.0001) when cultures were treated with a mixture comprising only 10% LPs and 40% CN extract. Furthermore, ATP-linked and FCCP-stimulated respiratory rates decreased by 62% (p = 0.0024) and 68% (p < 0.0001), respectively under the same conditions. A potentially synergistic antifungal effect of cyclic LPs with a CN extract is suggested.

show abstract

The antagonistic mechanism of rhizosphere microbes and endophytes on the interaction between banana and Fusarium oxysporum f. sp. cubense

Cited by 13 publications

References 93 publications

The core bacterial microbiome of banana (Musa spp.)

The core bacterial microbiome of banana (Musa spp.)

Isolation, identification, and genome of a Bacillus velezensis JIN4 exhibiting biocontrol activity against kiwifruit bacterial canker

Impaired bioenergetic response mediates the antifungal effect of lipopeptides from Bacillus tequilensis EA-CB0015 and cinnamon extract against Fusarium oxysporum f. sp. cubense

Contact Info

Product

Resources

About