The antagonistic effect and protective efficacy of gram-positive probiotics Bacillus coagulans to newly identified pathogens Pseudomonas aeruginosa in crucian carp Carassius auratus gibelio

Ji, Tongwei; Cao, Yangyang; Cao, Qingsheng; Zhang, Yingying; Yang, Hui

doi:10.1016/j.aqrep.2022.101126

Cited by 3 publications

(2 citation statements)

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“…Te popularity of plant-derived antibacterial products has witnessed a rapid growth considering that they facilitate the healthy and sustainable development of aquaculture [9]. At present, Lactobacillus and Bacillus represent the most commonly used probiotics in aquaculture [10]. Dietary supplementation with L. plantarum has been observed to improve immunity, disease resistance, and survival in aquatic animals [11,12].…”

Section: Introductionmentioning

confidence: 99%

“…Dietary supplementation with L. plantarum has been observed to improve immunity, disease resistance, and survival in aquatic animals [11,12]. Furthermore, feeding B. coagulans to aquatic animals has been found to improve their growth, immune response, and antioxidant capability [10,13]. Using the combination of probiotics and traditional Chinese medicines to achieve intestinal microecological balance has received much attention from the aquaculture industry [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

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Synergistic Impact of Lactobacillus plantarum and Bacillus coagulans on Solid-State Fermentation of Astragalus and Effects of Fermentation Products on Disease Resistance of Crucian Carp (Carassius auratus)

Shi

Xue

et al. 2023

Aquaculture Research

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Dietary supplementation with fermentation products of Astragalus can enhance the immune function of aquaculture animals. We explored the synergistic effects of Lactobacillus plantarum and Bacillus coagulans on the solid-state fermentation of Astragalus to investigate whether feeding fermentation products to crucian carp can improve disease resistance. The optimal ratio of Astragalus + (L. plantarum + B. coagulans) (ALB), temperature, and number was 3 : 3, 37°C, and 1.0 × 108 CFU/g, respectively. After 48 h mixed fermentation, the number of probiotics increased to 2.2 × 109 CFU/g, pH decreased to 3.2, and high molecular weight proteins disintegrated into small molecular weight or soluble proteins. In addition, several holes appeared on the Astragalus surface. Compared with unfermented Astragalus, the content of Astragalus polysaccharide and calycosin increased by 3.82- and 1.31-fold, respectively, on fermentation and that of total Astragalus saponins decreased by 0.77-fold. Furthermore, on Cyprinid herpesvirus 2 (CyHV-2) and Aeromonas veronii (A. veronii) challenges, the cumulative survival rates of crucian carp fed common feed and ALB were 80.00% and 65.00%, respectively. Overall, feeding mixed fermentation products to crucian carp positively impacted their health and disease resistance. We believe that our results provide theoretical guidance for developing effective plant-based agents to prevent diseases in crucian carp.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synergistic Impact of Lactobacillus plantarum and Bacillus coagulans on Solid-State Fermentation of Astragalus and Effects of Fermentation Products on Disease Resistance of Crucian Carp (Carassius auratus)

Shi

Xue

et al. 2023

Aquaculture Research

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Growth response of Bacillus coagulans on TiO2 nanoparticles by RNA-Seq analysis of the customary probiotic strain

Mukherjee

Mitra

Sil

et al. 2023

Process Biochemistry

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Bacteria of genus Bacillus as antagonists of pathogens in aquaculture

Morozova

Gorovcov

Празднова

et al. 2023

Vestnik of Astrakhan State Technical University. Series: Fishing Industry

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Due to the increasing resistance of aquaculture pathogens to antibiotics, the search for new approaches to the control of infectious diseases is of particular importance. A promising approach is the development of probiotic preparations based on antagonist bacteria. The paper studied the antagonistic activity of Bacillus strains that are promising for developing probiotics for fish. The strains were isolated from the bottom sediments of the Don River in the area of the Donskoy Fish Reserve, as well as from the intestines of cyprinids of natural populations, namely, from silver carp (Cyprinus gibelio), roach (Rutilus heckelii), and bream (Abramis brama). The inhibitory effect of Bacillus strains against causative agents of aeromonosis, pseudomonosis and bacterial hemorrhagic septicemia was studied by the method of delayed antagonism. Aeromonas and Pseudomonas species pathogenic for fish were isolated from sturgeons, cyprinids and salmons grown in pond farms and recirculating aquaculture systems in the Krasnodar Region, Rostov Region and the Republic of Adygea. It has been shown that sensitivity to the action of bacilli is not only a species, but also a strain-specific trait among bacterial pathogens of fish. Five strains were identified that exhibited the maximum antagonistic activity against the studied pathogens, and their species identification was carried out by molecular biological and mass spectrometric methods. It has been established that the most pronounced antagonism is exhibited by strains of bacilli isolated from bottom sediments. The growth of all studied strains of fish pathogenic species of Pseudomonas was suppressed only the B. subtilis R4 strain. Thus, strains of aerobic spore-forming bacteria suitable for the creation of probiotic preparations for aquaculture objects have been isolated and characterized.

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The antagonistic effect and protective efficacy of gram-positive probiotics Bacillus coagulans to newly identified pathogens Pseudomonas aeruginosa in crucian carp Carassius auratus gibelio

Cited by 3 publications

References 46 publications

Synergistic Impact of Lactobacillus plantarum and Bacillus coagulans on Solid-State Fermentation of Astragalus and Effects of Fermentation Products on Disease Resistance of Crucian Carp (Carassius auratus)

Synergistic Impact of Lactobacillus plantarum and Bacillus coagulans on Solid-State Fermentation of Astragalus and Effects of Fermentation Products on Disease Resistance of Crucian Carp (Carassius auratus)

Growth response of Bacillus coagulans on TiO2 nanoparticles by RNA-Seq analysis of the customary probiotic strain

Bacteria of genus Bacillus as antagonists of pathogens in aquaculture

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