The analysis of morphological distortion during AFM study of cells

Wu, Yangzhe; Hu, Yi; Cai, Jiye; Ma, Shengqiang; Wang, Xiaoping; Chen, Yong

doi:10.1002/sca.20121

Cited by 22 publications

(14 citation statements)

References 14 publications

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“…In this process, changes in sample structure and tip environment would affect the measurement of compression elasticity in vitro. In sum, development of theory on micromechanics around tips, improvement of AFM in higher resolution, and stabilization of measurement environment should be discussed in the further studies, which will make this method much more valuable (Wu et al, 2008; Zhu et al, 2009b). …”

Section: Discussionmentioning

confidence: 99%

Topographic mapping and compression elasticity analysis of skinned cardiac muscle fibers in vitro with atomic force microscopy and nanoindentation

Zhu

Sabharwal

Kalyanasundaram

et al. 2009

Journal of Biomechanics

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Surface topography and compression elasticity of bovine cardiac muscle fibers in rigor and relaxing state has been studied with atomic force microscopy. Characteristic sarcomere patterns running along the longitudinal axis of the fibers were clearly observed, and Z-lines, M-lines, I-bands, and A-bands can be distinguished through comparing with TEM images and force curves. AFM height images of fibers had shown a sarcomere length of 1.22±0.02μm (n=5) in rigor with a significant 9% increase in sarcomere length in relaxing state (1.33±0.03μm, n=5), indicating that overlap move with the changing physiological conditions. Compression elasticity curves along with sarcomere locations have been taken by AFM compression processing. Coefficient of Z-line, I-band, Overlap, and M-line are 25±2pN/nm, 8±1pN/nm, 10±1pN/nm, and 17±1.5pN/nm respectively in rigor state, and 18±2.5pN/nm, 4±0.5pN/nm, 6±1pN/nm, and 11±0.5pN/nm respectively in relaxing state. Young’s Modulus in Z-line, I-band, Overlap, and M-line are 115±12kPa, 48±9kPa, 52±8kPa, and 90±12kPa respectively in rigor, and 98±10kPa, 23±4kPa, 42±4kPa, and 65±7kPa respectively in relaxing state. The elasticity curves has shown a similar appearance to the section analysis profile of AFM height images of sarcomere and the distance between adjacent largest coefficient and Young’s Modulus is equal to the sarcomere length measured from the AFM height images using section analysis, indicating that mechanic properties of fibers have a similar periodicity to the topography of fibers.

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Section: Discussionmentioning

confidence: 99%

Topographic mapping and compression elasticity analysis of skinned cardiac muscle fibers in vitro with atomic force microscopy and nanoindentation

Zhu

Sabharwal

Kalyanasundaram

et al. 2009

Journal of Biomechanics

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“…where z i is the height of i th point, z m -the mean height and N is the total number of data points in the scanned area [51,52]. By definition, this surface roughness is a morphologyrelated parameter.…”

Section: The Erythrocyte Cell Morphologymentioning

confidence: 98%

Procaine Effect on Human Erythrocyte Membrane Explored by Atomic Force Microscopy

Zdrenghea¹,

Tomoaia

Pop-Toader³

et al. 2011

CCHTS

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The procaine effect on human erythrocytes was investigated by atomic force microscopy (AFM) at three procaine concentrations, about 5 x 10(-7) M, 5 x 10(-5) M and 5 x 10(-4) M. The changes in surface morphology of erythrocyte membrane bring direct evidence on the procaine effect on the cell membrane at micro- and nanometer scale. AFM images of the control erythrocytes (without procaine) showed a well defined concave (donut) shape of cells. The structure of control erythrocytes membrane is featured by closely packed nanometer size intra-membranous particles. After the incubation of the fresh blood with increasing procaine concentrations, a progressive increase in both concave depth and surface roughness of erythrocyte membrane was observed. The particles (granules) of the membrane surface increased progressively with increasing procaine concentrations. The changes in the surface morphology of erythrocyte membrane can be associated with the enlargement of surface granules, due to the aggregation of membranous particles within the cell surface, and the domain structure formation induced by procaine. A large number of moderate elevations from 25 nm to almost 40 nm in lateral size were found to be rather uniformly distributed on the surface of whole erythrocytes at low and medium procaine concentrations, respectively. At the highest procaine concentration, the granules of about 80 nm to almost 90 nm lateral size were found to form rows rather well separated. These data are in substantial agreement with the published results obtained on membrane models in the presence of procaine.

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“…The scanning range, the image mode and the tip shape influence such cell characteristics as cellular volume and ultrastructural roughness (Wu et al, 2008). True morphology can be partially reconstructed using procedures based on tip shape estimation (Keller & Franke, 1993).…”

Section: Measurements Of Tall Featuresmentioning

confidence: 99%

“…A number of artifacts related to the study of cells were analyzed and the influence of different AFM parameters (scanning mode, tip shape) on measured parameters (cellular volume, particles height) were discussed (Wu et al, 2008). Artificially formed features can be divided into four categories: artifacts related to the tip, to the scanner, to the sample and artifacts related to the interaction between them.…”

Section: Introductionmentioning

confidence: 99%