The ameliorative effect of black soybean and purple sweet potato to improve sperm quality through suppressing reactive oxygen species (ROS) in type 2 diabetes mellitus rat (Rattus novergicus)

Abstract

Overproduction of reactive oxygen species (ROS) will promote oxidative damage and can induce reproductive disorder. A therapeutic strategy to reduce effects of reproductive disorder is by maintaining the balance between ROS and antioxidants. Black soybean (BSB) contains phenolic compounds and has a greater antioxidant capacity than yellow soybean. Purple sweet potato (PSP) also has antioxidant capacity, due to the presence of anthocyanin, but is low in protein. The combination of BSB and PSP is expected to limit symptoms of reproductive disorder through decreasing ROS levels, enhancing testosterone production, and improve sperm quality. The results of this study indicate that the combination of PSP and BSB could restore blood glucose levels, improve seminiferous tubules diameter, decrease ROS production, and improve testosterone and sperm quality. A ratio of BSB to PSP of 2:2 and 3:1 were effective to improve symptoms of reproductive disorder in the type 2 diabetes mellitus (T2DM) rat model.

The Evaluation of Dietary Black Soybean and Purple Sweet Potato on Insulin Sensitivity in Streptozotocin - Induced Diabetic Rats

“…30 Our previous study suggests that BSB and PSP combination decline blood glucose level in T2DM rats. 31 In line with the result, the BSB and PSP combination improved cellular damage through the decrease of necrotic cells in the pancreas. Furthermore, the improves in pancreatic cells are followed by the improves of IRS-1 function.…”

The Combination of Black Soybean Tempeh and Purple Sweet Potato Affect Reactive Oxygen Species and Malondialdehyde Level in Streptozotocin-Induced Diabetic Rats

“…Flavonoids contained in BST can immediately chelate free radicals and act as scavenger by donating hydrogen atom then flavonoid radical will react with the second free radical and form a stable quinone (Gofur et al, 2018). Anthocyanins contained in PSP can reduce ROS activity by donating electrons to free radicals from the hydroxyl group (-OH), which are attached to the phenolic ring.…”

Black soybean tempeh and purple sweet potato improve sperm quality in streptozotocin-induced diabetic rats

“…The DM indicated rats were further divided into seven groups with four rats for each group: DM, DM treated with glibenclamide (0.6 mg/kg BW), DM treated with BST, DM treated with PSP, and DM treated with a combination of BST and PSP in 1:3, 2:2, and 3:1 ratios (C1, C2, and C3, respectively). Treatments were given for 30 days [ 17 ]. The long period of treatment was chosen asspermatogonia and spermatocytes in the seminiferous tubules has effect over agent exposure during spermatogenesis stages on 28+3 days [ 19 ].…”

“…Purple sweet potato (PSP) ( Ipomoea batatas L.; PSP) contains high anthocyanin and antioxidant properties that can improve blood and various organ damages caused by oxidative stress [ 15 , 16 ]. Our previous study suggested that non-fermented black soybean improves sperm quality in DM rats when combined with PSP [ 17 ]; however, limited information is available regarding the impact of black soybean tempeh (BST) on the sperm quality of T2DM individuals when combined with PSP. The antioxidant contents in both BST and PSP are expected to be therapeutic agents that can be used in DM treatments.…”