The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia

Abstract

Some studies on the hyperuricemia (HUA) have focused on intestinal bacteria. To better understand the correlation between gut microbiota and HUA, we established a HUA rat model with high-purine diet, and used 16S rRNA genes sequencing to analyze gut microbiota changes in HUA rats. To analyze the potential role played by gut microbiota in HUA, we altered the gut microbiota of HUA rats with antibiotics, and compared the degree of uric acid elevation between HUA and antibiotic-fed HUA rats (Ab+HUA). Finally, we established a recipient rat model, in which we transplanted fecal microbiota of HUA and normal rats into recipient rats. Three weeks later, we compared the uric acid content of recipient rats. As a result, the diversity and abundance of the gut microbiota had changed in HUA rats. The Ab-fed HUA rats had significantly lower uric acid content compared to the HUA rats, and gut microbiota from HUA rats increased uric acid content of recipient rats. The genera Vallitalea, Christensenella and Insolitispirillum may associate with HUA. Our findings highlight the association between gut microbiota and HUA, and the potential role played by gut microbiota in HUA. We hope that this finding will promote the isolation and culture of HUA-related bacteria and orient HUA-related studies from being correlational to mechanistic. These steps will therefore make it possible for us to treat HUA using gut microbiota as the target.

Effect of a Traditional Chinese Medicine Formula (CoTOL) on Serum Uric Acid and Intestinal Flora in Obese Hyperuricemic Mice Inoculated with Intestinal Bacteria

“…In recent years, many studies have found that the intestinal flora of patients with hyperuricemia is different from that of normal people. Compared with the intestinal flora of healthy people, hyperuricemia patients have a flora disorder, mainly manifested in the decrease of the number of Lactobacillus , while the number of Bacillus , Escherichia coli , and total aerobic bacteria increased [ 32 , 33 ]. Xanthine oxidase plays an important role in the formation of uric acid.…”

Swine gut microbiota and its interaction with host nutrient metabolism

“…The gut microbiota regulates the metabolism of glucolipids ( Yin et al., 2018 ), amino acids ( Kawase et al., 2017 ; Zhang et al., 2020 ), vitamins ( Caesar, 2019 ), bile acids ( Ramírez-Pérez et al., 2018 ), and other nutrients ( Pathak et al., 2020 ) through the microbiota–gut–brain axis ( Carabotti et al., 2015 ). Conversely, the dysbiosis of gut microbiota leads to metabolic diseases, such as obesity, cardiovascular disease (hyperlipidemia, atherosclerosis and hypertension), insulin resistance, type 2 diabetes, gout, and hyperuricemia ( Fan et al., 2018 ; Liu et al., 2020 ; Mandal and Mount, 2015 ; Mendes-Soares et al., 2019 ; Novoseletskyi, 2013 ). Fortunately, the homeostasis of gut microbiota can be manipulated by diet, faecal microbiota transplantation, and other approaches ( Fuentes et al., 2017 ; Kelly et al., 2014 ; Liu et al., 2017 ; Lee et al., 2019 ; Thomas and Versalovic, 2010 ).…”

Interrogation of the Perturbed Gut Microbiota in Gouty Arthritis Patients Throughin silicoMetabolic Modeling

“…Gouty arthritis is a chronic inflammatory joint disease that results from imbalanced purine metabolism in the human host. Recent findings that the gut microbiota are perturbed by gout disease (30,(32)(33)(34)(35)(36) xanthine), foods rich in hypoxanthine such as animal and fish meats have been reported to be more strongly associated with gout development (50). This uric acid precursor was elevated in the low All rights reserved.…”

“…The copyright holder for this preprint this version posted September 3, 2020. ; https://doi.org/10.1101/2020.09.02.20187013 doi: medRxiv preprint and murine models (32)(33)(34)(35) correlated changes in gut microbiota composition to the presence of gout disease, suggesting that microbiota properties could be used to monitor disease development, progression and recovery. Several of these 16S-based studies have been combined with gene catalog (36) and metabolomic (29) analyses to better understand the metabolic changes that accompanied compositional dysbiosis.…”

“…This in silico computational study was based on the hypothesis that altered gut microbiota were the result rather than the cause of gout disease, as a causative role has not been demonstrated to date. Indeed, uric acid in mainly produced in the liver by nucleic acid catabolism and only about 20% of uric acid production occurs from digestion of purine-rich foods (32)(33)(34)(35). Furthermore, only about 30% of host generated uric acid is secreted into and excreted out of the intestine, with the remainder is excreted through the kidneys (35,37).…”