The alpha and beta subchain of Amb a 1, the major ragweed-pollen allergen show divergent reactivity at the IgE and T-cell level

Wopfner, Nicole; Jahn‐Schmid, Beatrice; Schmidt, Georg; Christ, Tanja; Hubinger, Gudrun; Briza, Peter; Radauer, Christian; Bohle, Barbara; Vogel, Lothar; Ebner, Christof; Asero, Riccardo; Ferreira, Fátima; Schwarzenbacher, Robert

doi:10.1016/j.molimm.2009.02.005

Cited by 30 publications

(30 citation statements)

References 29 publications

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“…We have demonstrated previously that natural and recombinant proteins possess comparable T cell stimulation capacity (17). PBMCs (2 3 10 5 ) were stimulated in triplicates with concentrations from 0.3 to 20 mg/ml rAmb a 1 or rArt v 6.…”

Section: Allergen-specific T Cell Culturesmentioning

confidence: 98%

“…Recombinant Amb a 1 (rAmb a 1; Amb a 1.3, GenBank accession no. M62961, http://www.ncbi.nlm.nih.gov/nuccore/M62961, Amb a 1.3 clone R2 with 3 aa substitutions L23Y, F364L, H367R) (17) was obtained from Biomay (Vienna, Austria). Recombinant Art v 6 (rArt v 6) was expressed in Escherichia coli and purified as previously described (19).…”

Section: Allergensmentioning

confidence: 99%

“…The prevalence of sensitization to ragweed pollen has been increasing (1,2). In Austria, it increased from 8.5% in 1997 to 17.5% of pollen allergies in 2007 (3), and around Milan (Italy), ragweed today is the second most frequent cause of respiratory allergy next to grass pollen (1). The major allergen in ragweed pollen, Amb a 1, is a highly allergenic molecule that is recognized by .90% of ragweed-sensitized individuals (4,5) and accounts for .90% of the allergenic activity in ragweed pollen (6).…”

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confidence: 99%

“…Amb a 1 is another conceivable candidate molecule for causing cross-reactivity, because mugwort pollen contains a homologous allergen, Art v 6, with 65% amino acid sequence identity and 85% amino acid similarity (12,13,15). Previous attempts to investigate IgE cross-reactivity of Amb a 1 and Art v 6 have been hampered by the lack of properly folded, IgE-reactive recombinant or natural molecules (5,16,17). Therefore, we intended to define the IgE cross-reactivity between Amb a 1 and Art v 6 by using the purified natural weed pollen allergens.…”

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confidence: 99%

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Humoral and Cellular Cross-Reactivity between Amb a 1, the Major Ragweed Pollen Allergen, and Its Mugwort Homolog Art v 6

Jahn‐Schmid

Hauser

Wopfner

et al. 2012

The Journal of Immunology

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Ragweed and mugwort are closely related weeds that represent the major cause of pollen allergy in late summer. Concomitant sensitization and clinical cross-reactivity frequently occur in subjects who are coexposed to both pollen species, and have implications for diagnosis and specific immunotherapy. Molecules involved in this cross-reactivity might be Amb a 1, the major ragweed pollen allergen, and Art v 6, a highly homologous allergen from mugwort. Therefore, we investigated the IgE and T cell response to Art v 6 of 60 weed pollen-allergic patients and assessed its immunological cross-reactivity with Amb a 1. Results of ELISA inhibition experiments suggested that both allergens are largely cross-reactive, but Amb a 1 possesses more IgE epitopes than Art v 6. In patients with IgE to both allergens, Amb a 1-induced T cell lines and clones responded weakly to Art v 6. Moreover, Art v 6-induced T cell lines responded stronger to Amb a 1. T cell epitope mapping of Art v 6 revealed that it contains only a few cross-reactive epitopes, which is opposed to the multiple T cell-activating regions present in Amb a 1. In summary, Amb a 1 can elicit more diverse allergen-specific IgE and T cell responses than Art v 6 and dominates the cross-reactivity with its homolog. Nevertheless, Art v 6 can act as a primary sensitizing allergen in areas with high mugwort pollen exposure, and consequently may facilitate sensitization to Amb a 1 by epitope cross-recognition of T and B cells.

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Section: Allergen-specific T Cell Culturesmentioning

confidence: 98%

Section: Allergensmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Humoral and Cellular Cross-Reactivity between Amb a 1, the Major Ragweed Pollen Allergen, and Its Mugwort Homolog Art v 6

Jahn‐Schmid

Hauser

Wopfner

et al. 2012

The Journal of Immunology

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“…Furthermore, Amb a 1 is an acidic non-glycosylated protein with 397 amino acids (AA) and has a molecular weight of approximately 38 kDa [40]. Disulfide bonds can be located between C54-C71 and C211-C235, and potentially between C391 and C397 [41]. …”

Section: Major Ragweed Pollen Allergensmentioning

confidence: 99%

Ragweed Pollen Allergy: Burden, Characteristics, and Management of an Imported Allergen Source in Europe

Chen

Marusciac

Tamas

et al. 2018

Int Arch Allergy Immunol

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Ambrosia artemisiifolia, also known as common or short ragweed, is an invasive annual flowering herbaceous plant that has its origin in North America. Nowadays, ragweed can be found in many areas worldwide. Ragweed pollen is known for its high potential to cause type I allergic reactions in late summer and autumn and represents a major health problem in America and several countries in Europe. Climate change and urbanization, as well as long distance transport capacity, enhance the spread of ragweed pollen. Therefore ragweed is becoming domestic in non-invaded areas which in turn will increase the sensitization rate. So far 11 ragweed allergens have been described and, according to IgE reactivity, Amb a 1 and Amb a 11 seem to be major allergens. Sensitization rates of the other allergens vary between 10 and 50%. Most of the allergens have already been recombinantly produced, but most of them have not been characterized regarding their allergenic activity, therefore no conclusion on the clinical relevance of all the allergens can be made, which is important and necessary for an accurate diagnosis. Pharmacotherapy is the most common treatment for ragweed pollen allergy but fails to impact on the course of allergy. Allergen-specific immunotherapy (AIT) is the only causative and disease-modifying treatment of allergy with long-lasting effects, but currently it is based on the administration of ragweed pollen extract or Amb a 1 only. In order to improve ragweed pollen AIT, new strategies are required with higher efficacy and safety.

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Complex IgE sensitization patterns in ragweed allergic patients: Implications for diagnosis and specific immunotherapy

Buzan

Zbîrcea

Gattinger

et al. 2022

Clinical & Translational All

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Background Ragweed (Ambrosia artemisiifolia) is one of the most important allergen sources, worldwide, causing severe respiratory allergic reactions in late summer and fall, in sensitized patients. Amb a 1 has been considered as the most important allergen in ragweed but 12 ragweed pollen allergens are known. The aim of our study was to investigate IgE reactivity profiles of ragweed allergic patients and to associate them with clinical symptoms. Methods IgE sensitization profiles from clinically well‐characterized ragweed allergic patients (n = 150) were analyzed using immunoblotted ragweed pollen extract. Immunoblot inhibition experiments were performed with two Amb a 1 isoforms and CCD markers and basophil activation experiments were performed with IgE serum before and after depletion of Amb a 1‐specific IgE. Results By IgE‐immunoblotting 19 different IgE reactivity patterns with and without Amb a 1‐sensitization were found. The majority of patients (>95%) suffered from rhino‐conjunctivitis, around 60% reported asthma‐like symptoms and about 25% had skin reactions. Patients with complex IgE sensitization profiles tended to have more clinical symptoms. Serum with and without Amb a 1‐specific IgE induced basophil activation. Conclusions Ragweed pollen allergic patients exhibit complex IgE reactivity profiles to ragweed allergens including Amb a 1 isoforms and cross‐reactive carbohydrates indicating the importance of Amb a 1 isoforms and additional allergens for diagnosis and allergen‐specific immunotherapy of ragweed allergy.

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The alpha and beta subchain of Amb a 1, the major ragweed-pollen allergen show divergent reactivity at the IgE and T-cell level

Cited by 30 publications

References 29 publications

Humoral and Cellular Cross-Reactivity between Amb a 1, the Major Ragweed Pollen Allergen, and Its Mugwort Homolog Art v 6

Humoral and Cellular Cross-Reactivity between Amb a 1, the Major Ragweed Pollen Allergen, and Its Mugwort Homolog Art v 6

Ragweed Pollen Allergy: Burden, Characteristics, and Management of an Imported Allergen Source in Europe

Complex IgE sensitization patterns in ragweed allergic patients: Implications for diagnosis and specific immunotherapy

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