The allelopathic, cytotoxic and genotoxic effect of <i>Ambrosia artemisiifolia</i> on the germination and root meristems of <i>Zea mays</i>

Bonea, Dorina; Bonciu, Elena; Niculescu, M.; Olaru, Aurel Liviu

doi:10.1080/00087114.2017.1400263

Cited by 18 publications

(16 citation statements)

References 15 publications

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“…Different species of Ambrosia have some inhibitory effects on other plant crops, as revealed by their effect on seed germination and seedling growth of Zea mays (Bonea et al 2018), length of the roots of Helianthus annuus (Buzhdygan and Baglei 2016) and the growth and root hair development of Triticum aestivum (Kong et al 2007).…”

Section: Toxic Plantsmentioning

confidence: 99%

An evaluation for the standardization of theAllium cepatest as cytotoxicity and genotoxicity assay

Firbas²,

et al. 2018

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A general report on the use of the Allium test as cytotoxicological and genotoxicological assay is proposed, with particular emphasis about the standardization of the test in several common applications. The intraspecific variation in Allium cepa has been overlooked, as in most investigations no mention is made about origin and denomination of the onion cultivar used. A standardization of the used material would allow a better generalization of the results, since we cannot be sure that all cultivars would give the same response. A more frequent use of transmission electron microscopy (TEM) investigation is proposed. Even if relatively time consuming and not available in all laboratories, it may help to better understand the mechanism of cytotoxicity, since many morphological characters may appear similar but be arisen from different processes observable only with TEM. About statistical testing, tests other than chi-squared may be used in case of a lower amount of data. The most commonly used statistical tests are the parametric tests ANOVA and Student's t, and the nonparametric tests Kruskal-Wallis and Mann-Whitney U, for analysis of variance. Tests should be used also to assess the minimal sample dimension for obtaining significance, since data collection (microscope observation) appears to be one of the main bottle necks of the test. Also the use of the Allium test for testing liposomes and other nanovectors for drug delivery is proposed, in order to assess the cytotoxicity of these types of medium and the possible increase in cytotoxicity of the associated drug.

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Section: Toxic Plantsmentioning

confidence: 99%

An evaluation for the standardization of theAllium cepatest as cytotoxicity and genotoxicity assay

Firbas²,

et al. 2018

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“…The reason is not only that A. artemisiifolia has strong growth characteristics, but also its successful allelopathic effects on native plants. Previous studies reported that various solvent extracts of A. artemisiifolia had significant inhibitory effects on the growth and seed germination of Lactuca sativa, Lycopersicon esculentum, Zea mays, and other plants (Lehoczky et al, 2011;Vidotto et al, 2013;Bonea et al, 2018). These studies also confirmed that allelopathy is a major reason for the successful invasion of A. artemisiifolia.…”

Section: Introductionmentioning

confidence: 53%

Sesquiterpenes from Ambrosia artemisiifolia and their allelopathy

Liu

Zhang

et al. 2022

Front. Plant Sci.

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Ambrosia artemisiifolia, an invasive plant, has seriously harmed the agricultural production, native ecosystems and human health. Allelopathy is an important reason for the successful invasion of this alien plant. However, the chemical basis, action effects, action mechanism and release pathway of its allelopathy remain unclear. To address these problems, four sesquiterpenes (1–4), consisting of three new sesquiterpenes (1–2, 4), were isolated from the whole plant of A. artemisiifolia using a variety of column chromatography techniques, and identified using HR-ESIMS, 1D-NMR, 2D-NMR, and ECD. All the compounds exhibited different levels of inhibitory effects on three native plants (Setaria viridis, Digitaria sanguinalis, Chenopodium album) and one model plant (Arabidopsis thaliana), especially compound 1. In addition, the preliminary action mechanism of active compound 1 was revealed by FDA/PI staining assay. Furthermore, the allelopathic substances 1–3 were released into environment through the root secretion pathway by UPLC-MS/MS analyses.

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“…Polyploidy refers to a numerical change in an entire set of chromosomes (Grant and Salamone, 1994). Giant cells are usually polyploid and result of endomitosis or endoreplication (Bonea et al, 2018). It is obvious that the reactive short-lived products of radon decay, and radiation they emit, acted directly or indirectly on the genetic material, and disrupted the structure of the mitotic spindle, and caused its dysfunction.…”

Section: Resultsmentioning

confidence: 99%

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2021

Int. J. Mod. Biol. Res.

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In this study, the Allium biotest was used to assess the cytotoxic and genotoxic potential of radon activity concentrations in water, on meristematic cells of Allium cepa L. roots. To identify the biological effects of ionizing radiation, which come from the radioactive gas radon, it was necessary to take into account its physicochemical properties, primarily considering its migratory possibilities. The chromosomes studied were taken from meristematic cells, whose normal cells have 16 (2n = 16) chromosomes, and are suitable lengths for detecting morphological changes. Meristematic cells of A. cepa L. root were treated with a series of 10 different radon activity concentrations in water, ranging from 100.62 to 1,006.25 Bq/L. From a wide range of observed chromosomal aberrations (CA) and nuclear changes, the highest frequency of occurrence was: polyploidy, sticky chromosomes, C-mitosis, bridges, micronuclei, irregular segregation, multipolarity, necrosis, apoptosis, binuclear cells. Trinuclear and tetranuclear cells, as well as stray chromosomes, were observed sporadically. In relation to the negative control, all applied radon activity concentrations in water led to statistically significant genotoxic and cytotoxic effects on meristematic cells of A. cepa L.

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The allelopathic, cytotoxic and genotoxic effect of Ambrosia artemisiifolia on the germination and root meristems of Zea mays

Cited by 18 publications

References 15 publications

An evaluation for the standardization of theAllium cepatest as cytotoxicity and genotoxicity assay

An evaluation for the standardization of theAllium cepatest as cytotoxicity and genotoxicity assay

Sesquiterpenes from Ambrosia artemisiifolia and their allelopathy

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