“…Indeed, acetylcholine by binding to its muscarinic type receptors on heart, could cause, negative inotropic and chronotropic effects (Felder, 1995;Bois et al, 1999;Roffel et al, 2001;Racke and Matthiesen, 2004;Gerova et al, 2005;Hirota and McKay, 2006) and the vasorelaxation by releasing nitric oxide (NO) by endothelium (Furchgott and Zawadzki, 1980;Zapol et al, 1994;Ducrocq et al, 2001;Zhao et al, 2004;Lamblin et al, 2005;Collin and Levy, 2008;Jeanneret, Sanchez, Liaudet, 2011).…”