The COVID-19 pandemic jeopardized the traditional academic learning calendars due to the closing of all educational institutions across the globe. To keep up with the flow of learning most of the educational institutions shifted toward e-learning. However, the questions of the students e-learning preference for various sub-domains of e-learning readiness did not identify, particularly among the female nursing students for a developing country like Bangladesh, where those domains pose serious challenges. A cross-sectional study was conducted among the female nursing students perceived e-learning readiness in sub-domains of readiness; availability of technology, use of technology, self-confidence, and acceptance. About 237 nursing students were recruited, who have enrolled in e-learning at least the last 30 days of the participation. A multivariable linear regression model was fitted to find the association between students preference and the perceived e-learning readiness with demographic and e-learning related factors. The findings of the study revealed that more than half of the students, 56.54% (n=134) did not prefer e-learning. The students did not prefer e-learning compared to prefer group has significantly less availability of technology (β = -3.01, 95% CI: -4.46, -1.56), less use of technology (β = -3.08, 95% CI: -5.11, -1.06), less self-confidence (β = -4.50, 95% CI: -7.02, -1.98), and less acceptance (β = -5.96, 95% CI: -7.76, -4.16). The age, degree, residence, parents highest education, having a single room, having any eye problems significantly associated with the variation of availability of technology, use of technology, self-confidence, and acceptance for e-learning. The outcomes of the study could be helpful while developing an effective and productive e-learning infrastructure regarding the preparedness of nursing colleges for the continuation of academia in any adverse circumstances like the COVID-19 pandemic.