The additive free microwave hydrolysis of lignocellulosic biomass for fermentation to high value products

Fan, Jiajun; Santomauro, Fabio; Budarin, Vitaliy L.; Whiffin, Fraeya; Abeln, Felix; Chantasuban, Tanakorn; Gore‐Lloyd, Deborah; Henk, Daniel A.; Scott, Roderick J.; Clark, James H.; Chuck, Christopher J.

doi:10.1016/j.jclepro.2018.07.088

Cited by 35 publications

(49 citation statements)

References 27 publications

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“…Metschnikowia pulcherrima is a relatively under‐explored oleaginous yeast (Abeln et al, ; Canonico et al, ; Santomauro, Whiffin, Scott, & Chuck, ), however, the yeast is an ideal organism for industrial biotechnology due to: elevated inhibitor tolerance: specifically, formic acid, acetic acid, furfural, hydroxymethylfurfural (HMF) in concentrations up to 10 g L −1 (Fan et al, ; Sitepu, Selby, Lin, Zhu, & Boundy‐Mills, ); a wide substrate spectrum: a range of C 5 and C 6 monosaccharides and oligosaccharides (Abeln et al, ; Fan et al, ; Santomauro et al, ; Sitepu et al, ); production of an array of secondary metabolites: most prominently 2‐phenylethanol (2‐PE), a valuable fragrance compound (Abeln et al, ; Chantasuban et al, ); its antimicrobial activity: secretion of antimicrobial agents such as 2‐PE and iron sequestration through pulcherrimin production (Gore‐Lloyd et al, ; Krause et al, ; Oro, Ciani, & Comitini, ; Sipiczki, ; Türkel, Korukluoğlu, & Yavuz, ); being nonpathogenic: it is commonly used in wine fermentations as often found on grapes (Barbosa et al, ; Clemente‐Jimenez, Mingorance‐Cazorla, Martínez‐Rodríguez, Las Heras‐Vázquez, & Rodríguez‐Vico, ; Oro et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…elevated inhibitor tolerance: specifically, formic acid, acetic acid, furfural, hydroxymethylfurfural (HMF) in concentrations up to 10 g L −1 (Fan et al, ; Sitepu, Selby, Lin, Zhu, & Boundy‐Mills, );…”

Section: Introductionmentioning

confidence: 99%

“…a wide substrate spectrum: a range of C 5 and C 6 monosaccharides and oligosaccharides (Abeln et al, ; Fan et al, ; Santomauro et al, ; Sitepu et al, );…”

Section: Introductionmentioning

confidence: 99%

“…These characteristics combined mean it can grow under nonaseptic conditions on a wide range of different substrates including inhibitor‐rich lignocellulosic hydrolysates and waste streams (Abeln et al, ; Fan et al, ), making it an outstanding candidate for low‐cost lipid production (Santomauro et al, ). Recently, promising improvements have also been made to enhance lipid production, whilst achieving beyond 40% (w/w) lipids similar in composition to palm oil.…”

Section: Introductionmentioning

confidence: 99%

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Achieving a high‐density oleaginous yeast culture: Comparison of four processing strategies using Metschnikowia pulcherrima

Abeln

Chuck

2019

Biotech & Bioengineering

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Microbial lipids have the potential to displace terrestrial oils for fuel, value chemical, and food production, curbing the growth in tropical oil plantations and helping to reduce deforestation. However, commercialization remains elusive partly due to the lack of suitably robust organisms and their low lipid productivity. Extremely high cell densities in oleaginous cultures are needed to increase reaction rates, reduce reactor volume, and facilitate downstream processing. In this investigation, the oleaginous yeast Metschnikowia pulcherrima, a known antimicrobial producer, was cultured using four different processing strategies to achieve high cell densities and gain suitable lipid productivity. In batch mode, the yeast demonstrated lipid contents more than 40% (w/w) under high osmotic pressure. In fed-batch mode, however, high-lipid titers were prevented through inhibition above 70.0 g L −1 yeast biomass. Highly promising were a semi-continuous and continuous mode with cell recycle where cell densities of up to 122.6 g L −1 and maximum lipid production rates of 0.37 g L −1 h −1 (daily average), a nearly two-fold increase from the batch, were achieved. The findings demonstrate the importance of considering multiple fermentation modes to achieve high-density oleaginous yeast cultures generally and indicate the limitations of processing these organisms under the extreme conditions necessary for economic lipid production. K E Y W O R D Scontinuous, fed-batch, high cell density, microbial lipids, oleaginous yeast, semi-continuous

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…a wide substrate spectrum: a range of C 5 and C 6 monosaccharides and oligosaccharides (Abeln et al, ; Fan et al, ; Santomauro et al, ; Sitepu et al, );…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Achieving a high‐density oleaginous yeast culture: Comparison of four processing strategies using Metschnikowia pulcherrima

Abeln

Chuck

2019

Biotech & Bioengineering

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“…Genetically modified Y. lipolytica has been cultured at low carbon concentrations in semi‐continuous operation rather than under nitrogen starvation achieving a lipid productivity of 0.80 g L −1 h −1 – also on acetate . Such strategies are not required with M. pulcherrima , which – as shown on multiple substrates – still accumulates considerable amounts of lipids on nutrient‐rich feedstock (Fig. , Figs S3 and S4, Table ).…”

Section: Discussionmentioning

confidence: 99%

The role of temperature, pH and nutrition in process development of the unique oleaginous yeast Metschnikowia pulcherrima

Abeln

Chuck

2020

J of Chemical Tech & Biotech

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BACKGROUND Lipids produced from oleaginous yeasts are a promising alternative to terrestrial oils. Despite promising cellular yields of lipid however, an industrial process remains elusive. One key processing bottleneck is the need to provide nutrient‐rich conditions for cellular growth and then extended nutrient‐depleted conditions for lipid accumulation. Surprisingly, investigations detailing process development, particularly with a focus on kinetics, are rare in this field. RESULTS In this investigation we report on the unique oleaginous yeast Metschnikowia pulcherrima, where lipid accumulation ≥ 29.8% (w/w) was achieved without apparent nutrient limitation. The process was developed in stirred tank reactors through determining the influence of temperature, pH and nutrition on lipid production. A temperature of up to 25 °C and initial pH 5 could be applied to enhance initial reaction kinetics. Through the increased supply of yeast extract of up to 5% (w/w) of glucose, a maximum lipid production rate of 0.60 g L−1 h−1 (4 h‐average), productivity of 0.29 g L−1 h−1, and yield of 0.17 g g−1 glucose were achieved – the highest yet recorded with this yeast. Suitable to combat the excessive secretion of polyols of up to 0.11 g g−1 glucose was an excess nutrient supply as well as a low cultivation temperature of 15 °C and moderate pH 5. CONCLUSIONS This study demonstrates that the lack of a starvation stage, coupled with effective process development is required for oleaginous yeasts to achieve the yields and productivities required for commercial lipid production. © 2019 Society of Chemical Industry

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Biomass Processing via Microwave Treatment

Rosa

Cravotto

Leonelli

2021

Biomass Valorization

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The additive free microwave hydrolysis of lignocellulosic biomass for fermentation to high value products

Cited by 35 publications

References 27 publications

Achieving a high‐density oleaginous yeast culture: Comparison of four processing strategies using Metschnikowia pulcherrima

Achieving a high‐density oleaginous yeast culture: Comparison of four processing strategies using Metschnikowia pulcherrima

The role of temperature, pH and nutrition in process development of the unique oleaginous yeast Metschnikowia pulcherrima

Biomass Processing via Microwave Treatment

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