The acute inflammatory response induced in mice by the venom of the giant ant Dinoponera quadriceps involves macrophage and interleukin-1β

Sousa, Paloma Leão; Quinet, Yves; Brizeno, Luiz André Cavalcante; Sampaio, Tiago Lima; Torres, Alba; Martins, Alice Maria Costa; Assreuy, Ana Maria Sampaio

doi:10.1016/j.toxicon.2016.03.009

Cited by 15 publications

(9 citation statements)

References 39 publications

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“…Recently, many studies using animal models in high fat and cholesterol diet showed a remarkable increase in circulating levels of lipocalin 2 and its hepatic expression. These modifications were associated with increased infiltration of neutrophils [27]. Therefore, neutrophils seem to get activated and then migrate towards high-fat concentration sites in vivo .…”

Section: Discussionmentioning

confidence: 99%

Samsum ant venom modulates the immune response and redox status at the acute toxic dose in vivo

Ebaid

Abdel-Salam

Alhazza

et al. 2019

J. Venom. Anim. Toxins incl. Trop. Dis

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Background: Ant venoms express surface molecules that participate in antigen presentation involving pro- and anti-inflammatory cytokines. This work aims to investigate the expression of MHC-II, CD80 and CD86 on the polymorphonuclear cells (PMNs) in rats injected with samsum ant venom (SAV).Methods: Rats were divided into three groups - control, SAV-treated (intraperitoneal route, 600 μg/kg), and SAV-treated (subcutaneous route, 600 μg/kg). After five doses, animals were euthanized and samples collected for analysis.Results: The subcutaneous SAV-trated rats presented decreased levels of glutathione with increased cholesterol and triglyceride levels. Intraperitoneal SAV-treated animals displayed significantly reduced concentrations of both IFN-γ and IL-17 in comparison with the control group. However, intraperitoneal and subcutaneous SAV-treated rats were able to upregulate the expressions of MHC-II, CD80 and CD86 on PMNs in comparison with the control respectively. The histological examination showed severe lymphocyte depletion in the splenic white pulp of the intraperitoneal SAV-injected rats.Conclusion: Stimulation of PMNs by SAV leads to upregulation of MHC-II, CD 80, and CD 86, which plays critical roles in antigen presentation and consequently proliferation of T-cells. Subcutaneous route was more efficient than intraperitoneal by elevating MHC-II, CD80 and CD86 expression, disturbing oxidative stability and increasing lipogram concentration.

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Section: Discussionmentioning

confidence: 99%

Samsum ant venom modulates the immune response and redox status at the acute toxic dose in vivo

Ebaid

Abdel-Salam

Alhazza

et al. 2019

J. Venom. Anim. Toxins incl. Trop. Dis

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“…We have found that SAV, at a low dose, is a promising source that offers potential biologically active properties [1, 23, 24] that may be useful as new tools for the design of drugs. Sousa et al [4] demonstrated that the inflammatory effect of venom of Dinoponera quadriceps characterized by edema, an increase in vascular permeability and neutrophil migration that implies the participation of resident macrophages and IL-1β, among other inflammatory mediators. Similarly, SAV is an important health concern in the habitats including Saudi Arabia, Middle East, and some Asian countries.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies reveal that either the whole venom at specific doses or some of its bioactive chemical compounds are promising therapeutic candidates against various diseases and allergies [2, 3]. Furthermore, Sousa et al [4] have demonstrated the inflammatory effect of Dinoponera quadriceps venom characterized by edema and enhanced vascular permeability concomitant with neutrophil migration implying the participation of resident macrophages and IL-1β in a rodent-based study. Also, Lima et al [5] recently reported a strong antimicrobial effect of SAV on the parasites.…”

Section: Introductionmentioning

confidence: 99%

Thymoquinone ameliorates Pachycondyla sennaarensis venom-induced acute toxic shock in male rats

Alhazza

Ebaid

Abdel-Salam

et al. 2019

BMC Pharmacol Toxicol

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BackgroundFor many decades, the sting of Samsun ant (Pachycondyla sennaarensis) has been a serious clinical challenge for the people living in some of the major Middle East and Asian countries. In the present study, the therapeutic potential of Nigella sativa derived plant extract component, thymoquinone (TQ) has been tested against the Samsun ant venom (SAV) at the toxic dose in the rats.MethodsThe adult male rats were divided into four groups (n = 10): control, SAV treated, SAV + TQ treated and TQ alone treated. It was found that the sub-lethal dose of SAV alters not only many of the kidney and liver function markers but also induces oxidative stress in the animals. Moreover, the SAV also disturbs various immunological parameters including expression of PMNs, CD-80, CD-86, interleukins and other cytokines compromising the affected organism towards mild to severe allergic reactions including life-risking anaphylaxis.ResultsThe plant extract, TQ, effectively restores many of the biochemical and oxidative stress parameters comparable to the normal concomitant with improving the immunological aspects that might attributive in relieving from SAV-induced toxicity and allergic reactions in the affected organism to a greater extent.ConclusionHence, TQ has an excellent antidote property against SAV-induced toxicities in vivo. Although the study is a vivid indication of the potential therapeutic potential of TQ against the SAV induced in vivo toxicity, yet the actual mechanism of interaction translating the toxicity amelioration warrants further investigations.

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“…Recently, other studies [ 69 , 70 ] tested the inflammatory activity of the venom using the paw edema and peritonitis models, showing a dose-dependent inflammatory response in the paw edema model and an increase in the number of leucocytes and interleukin-1β in the peritoneal fluid. In addition, the venom also showed anticoagulant and antiplatelet effects in vivo [ 69 ].…”

Section: Discussionmentioning

confidence: 99%

Anticonvulsant Effects of Fractions Isolated from Dinoponera quadriceps (Kempt) Ant Venom (Formicidae: Ponerinae)

Nôga

Brandão

Cagni

et al. 2016

Toxins

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Natural products, sources of new pharmacological substances, have large chemical diversity and architectural complexity. In this context, some toxins obtained from invertebrate venoms have anticonvulsant effects. Epilepsy is a neurological disorder that affects about 65 million people worldwide, and approximately 30% of cases are resistant to pharmacological treatment. Previous studies from our group show that the denatured venom of the ant Dinoponera quadriceps (Kempt) protects mice against bicuculline (BIC)-induced seizures and death. The aim of this study was to investigate the anticonvulsant activity of compounds isolated from D. quadriceps venom against seizures induced by BIC in mice. Crude venom was fractionated by high-performance liquid chromatography (HPLC) resulting in six fractions referred to as DqTx1–DqTx6. A liquid chromatography-mass spectrometry (LC/MS) analysis revealed a major 431 Da compound in fractions DqTx1 and DqTx2. Fractions DqTx3 and DqTx4 showed a compound of 2451 Da and DqTx5 revealed a 2436 Da compound. Furthermore, the DqTx6 fraction exhibited a major component with a molecular weight of 13,196 Da. Each fraction (1 mg/mL) was microinjected into the lateral ventricle of mice, and the animals were observed in an open field. We did not observe behavioral alterations when the fractions were given alone. Conversely, when the fractions were microinjected 20 min prior to the administration of BIC (21.6 nM), DqTx1, DqTx4, and DqTx6 fractions increased the latency for onset of tonic-clonic seizures. Moreover, all fractions, except DqTx5, increased latency to death. The more relevant result was obtained with the DqTx6 fraction, which protected 62.5% of the animals against tonic-clonic seizures. Furthermore, this fraction protected 100% of the animals from seizure episodes followed by death. Taken together, these findings indicate that compounds from ant venom might be a potential source of new anticonvulsants molecules.

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The acute inflammatory response induced in mice by the venom of the giant ant Dinoponera quadriceps involves macrophage and interleukin-1β

Cited by 15 publications

References 39 publications

Samsum ant venom modulates the immune response and redox status at the acute toxic dose in vivo

Samsum ant venom modulates the immune response and redox status at the acute toxic dose in vivo

Thymoquinone ameliorates Pachycondyla sennaarensis venom-induced acute toxic shock in male rats

Anticonvulsant Effects of Fractions Isolated from Dinoponera quadriceps (Kempt) Ant Venom (Formicidae: Ponerinae)

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