The Acute bee paralysis virus–Kashmir bee virus–Israeli acute paralysis virus complex

Miranda, Joachim R. de; Cordoni, Guido; Budge, Giles E.

doi:10.1016/j.jip.2009.06.014

Cited by 292 publications

(250 citation statements)

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“…Aside from the above mortality and sub-lethal effects, the symptoms associated with IAPV have not been well characterized and thus hinder our ability to observe the progression of IAPV infection in these important pollinators, especially when considering that virus-induced paralysis symptoms are normally the signal of overt infection and death. Besides, the initial observation of IAPV symptoms are from honeybees; symptom analysis in bumblebees would enhance our knowledge of this virus (de Miranda et al, 2010;DeGrandi-Hoffman & Chen, 2015;Maori et al, 2007Maori et al, , 2009Piot et al, 2015). Therefore, in this study, we report progressive viral symptoms in workers of B. terrestris, which is one of the most numerous bumblebee species in Europe (Velthuis & van Doorn, 2006).…”

Section: Introductionmentioning

confidence: 75%

“…IAPV is positive single-stranded RNA virus in the family Dicistroviridae with a global distribution range (de Miranda et al, 2010;Maori et al, 2007;Palacios et al, 2008). The nomenclature of this virus is largely based on its symptoms that are similar to ABPV.…”

Section: Introductionmentioning

confidence: 99%

“…DWV caused crippled wings in bumblebee (Bombus terrestris and Bombus pascuorum) workers even without Varroa (Fürst et al, 2014;Genersch et al, 2006). Interestingly, viral tissue tropism was different between DWV-infected (asymptomatic) and clinically diseased (symptomatic) honeybees and bumblebees, indicating that overt infection may also be associated with virus tissue spreading (Genersch et al, 2006).IAPV is positive single-stranded RNA virus in the family Dicistroviridae with a global distribution range (de Miranda et al, 2010;Maori et al, 2007;Palacios et al, 2008). The nomenclature of this virus is largely based on its symptoms that are similar to ABPV.…”

mentioning

confidence: 99%

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Israeli acute paralysis virus associated paralysis symptoms, viral tissue distribution and Dicer-2 induction in bumblebee workers (Bombus terrestris)

Wang

Meeus

Smagghe

2016

Journal of General Virology

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Although it is known that Israeli acute paralysis virus (IAPV) can cause bee mortality, the symptoms of paralysis and the distribution of the virus in different body tissues and their potential to respond with an increase of the siRNA antiviral immune system have not been studied. In this project we worked with Bombus terrestris, which is one of the most numerous bumblebee species in Europe and an important pollinator for wild flowers and many crops in agriculture. Besides the classic symptoms of paralysis and trembling prior to death, we report a new IAPVrelated symptom, crippled/immobilized forelegs. Reverse-transcriptase quantitative PCR showed that IAPV accumulates in different body tissues (midgut, fat body, brain and ovary). The highest levels of IAPV were observed in the fat body. With fluorescence in situ hybridization (FISH) we detected IAPV in the Kenyon cells of mushroom bodies and neuropils from both antennal and optic lobes of the brain in IAPV-infected workers. Finally, we observed an induction of Dicer-2, a core gene of the RNAi antiviral immune response, in the IAPV-infected tissues of B. terrestris workers. According to our results, tissue tropism and the induction strength of Dicer-2 could not be correlated with virus-related paralysis symptoms. INTRODUCTIONHoneybees and bumblebees are the two most important bee species used in modern apiculture and agriculture. They host many viruses. When looking at the honeybee, there have been around 23 viruses reported and many of these viruses only persist as covert and asymptomatic infections which show no detectable impact at both the individual and colony level (McMenamin & Genersch, 2015). However, under certain conditions, for example the presence of the ectoparasitic honeybee mite Varroa destructor, the covert infections of some viruses turn into overt ones with observable symptoms (Martin et al., 2012;McMahon et al., 2015;Singh et al., 2010). The symptoms of overt infections vary from virus to virus, including deformed wings, discoloration, hair losses, bloated abdomen, paralysis, decreased locomotion, impaired cognition and both brood and adult mortality (Lanzi et al., 2006;McMenamin & Genersch, 2015;Singh et al., 2010). Some symptoms can be easily recognized by the behaviour and appearance of the infected bees, while others can only be confirmed through meticulous and continuous observations or by molecular techniques.In bumblebees (Bombus spp.) a subset of honeybee-infecting viruses has been reported, such as deformed wing virus (DWV), Israeli acute paralysis virus (IAPV), acute bee paralysis virus (ABPV), Kashmir bee virus (KBV), slow bee paralysis virus (SBPV), sacbrood virus (SBV), black queen cell virus (BQCV) and Lake Sinai virus (LSV) (Genersch et al., 2006;Levitt et al., 2013;Parmentier et al., 2016;Peng et al., 2011). However, only DWV, KBV and IAPV have been reported to cause clinical symptoms (Genersch et al., 2006;Meeus et al., 2014). DWV caused crippled wings in bumblebee (Bombus terrestris and Bombus pascuorum) workers even without ...

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Section: Introductionmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Israeli acute paralysis virus associated paralysis symptoms, viral tissue distribution and Dicer-2 induction in bumblebee workers (Bombus terrestris)

Wang

Meeus

Smagghe

2016

Journal of General Virology

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“…In Europe, IAPV has been found in France (Blanchard et al, 2008) and more recently in Spain (Kukielka & Sánchez-Vizcaíno, 2010) and Poland (Pohorecka et al, 2011). However, the high frequencies found in some countries (up to 41% in Argentina) and lack of obvious disease symptoms in most sampled colonies suggest that IAPV is a widespread virus that usually appears in covert infections, like most Dicistroviruses (De Miranda et al, 2010). Criteria identification to differentiate covert from overt infections in honeybee colonies is essential to identify risk factors of honeybee diseases, including CCD, where viruses may play an important role.…”

mentioning

confidence: 99%

“…Another explanation for the covert infection of IAPV is the virulence of the isolate. Most published isolates are low-virulent, as IAPV normally persists in the colony in covert infections, showing no obvious symptoms at the individual or colony level (De Miranda et al, 2010). In Spain, only one sequence has been phylogenetically analysed from one healthy colony in Valencia (Kukielka & Sánchez-Vizcaíno, 2010 …”

mentioning

confidence: 99%

Short communication. Presence, quantification and phylogeny of Israeli acute paralysis virus of honeybees in Andalusia (Spain)

Vicente-Rubiano

Kukielka

Heras

et al. 2013

Span. j. agric. res.

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This study aimed to assess the possible relationship between the presence of Israeli acute paralysis virus (IAPV) of honeybees and disease symptoms development at the colony level, to describe the IAPV load in field colonies and to illustrate phylogenetic relationships between IAPV isolates in Andalusia (Spain). Presence and load of IAPV was studied in 96 colonies from all provinces in Andalusia. Epidemiological surveys were performed in all the colonies to assess their sanitary status. IAPV was found in 13.5% of the sampled colonies, and no association was observed between the presence of IAPV and disease symptoms at the colony level. An average IAPV load was established in 4.9•105 genome equivalent copies per bee. Phylogenetic analysis revealed that Andalusian isolates belong to a different lineage to a previously described isolate found in Valencia (2010). The results of this study will help us understand the epidemiology and effect of IAPV on Spanish colonies.

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Honey Bees

Vidal-Naquet¹,

Cripps²

2021

Invertebrate Medicine

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The Acute bee paralysis virus–Kashmir bee virus–Israeli acute paralysis virus complex

Cited by 292 publications

References 163 publications

Israeli acute paralysis virus associated paralysis symptoms, viral tissue distribution and Dicer-2 induction in bumblebee workers (Bombus terrestris)

Israeli acute paralysis virus associated paralysis symptoms, viral tissue distribution and Dicer-2 induction in bumblebee workers (Bombus terrestris)

Short communication. Presence, quantification and phylogeny of Israeli acute paralysis virus of honeybees in Andalusia (Spain)

Honey Bees

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