Short communication. Presence, quantification and phylogeny of Israeli acute paralysis virus of honeybees in Andalusia (Spain)

Vicente-Rubiano, Marina; Kukielka, Deborah; Heras, Ana Isabel de las; Sánchez‐Vizcaíno, José Manuel

doi:10.5424/sjar/2013113-3531

Cited by 5 publications

(6 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The total absence of IAPV during the entire study suggests that the virus is not abundant in Spain, at least not in the province of Córdoba. The fact that we can exclude IAPV as a cause of the total loss and depopulation of several colonies during the study is reminiscent of our 2013 observation, also in Andalusia, that IAPV was not involved with colony collapse in that study (Vicente-Rubiano et al, 2013). Our results suggest that the association between IAPV and CCD observed in the USA (Cox-Foster et al, 2007; Maori et al, 2007) may not apply to southern Spain.…”

Section: Discussionmentioning

confidence: 55%

“…It also damages honey bees indirectly by transmitting several viruses, including deformed wing virus (DWV), Kashmir bee virus (KBV), sacbrood bee virus (SBV), and Israeli acute paralysis virus (IAPV) (Kralj and Fuchs, 2006; Martin et al, 2012). Meta-genomic analysis has suggested that IAPV may cause CCD (Cox-Foster et al, 2007; Maori et al, 2007), but subsequent work in Spanish apiaries has called into question the link between IAPV and colony losses (Blanchard et al, 2008; Garrido et al, 2013; Vicente-Rubiano et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

High Load of Deformed Wing Virus and Varroa destructor Infestation Are Related to Weakness of Honey Bee Colonies in Southern Spain

et al. 2019

View full text Add to dashboard Cite

Many factors, including pathogens, contribute to the continuing losses of colonies of the honey bee Apis mellifera , which has led to steady population decline. In particular, colony losses have been linked to deformed wing virus (DWV) and the Varroa destructor mite. To clarify the potential role of these two pathogens in honey bee colony weakening and loss, we sampled colonies in southern Spain during a 21-month period and analyzed the samples for loads of four viruses and varroa. Loads of DWV and black queen cell virus as well as varroa infestation negatively correlated with colony vigor as measured using the subjective colony strength method. Logistic regression identified varroa and DWV as the main factors involved in colony weakening. Our results confirm that varroa and DWV play a key role in triggering colony weakening in southern Spain and provide evidence that experienced beekeepers’ and technicians’ assessments of colony vigor can accurately estimate colony strength.

show abstract

Section: Discussionmentioning

confidence: 55%

Section: Introductionmentioning

confidence: 99%

High Load of Deformed Wing Virus and Varroa destructor Infestation Are Related to Weakness of Honey Bee Colonies in Southern Spain

et al. 2019

View full text Add to dashboard Cite

show abstract

“…IAPV and SBV were not detected in any colony during the entire study. Although one study has associated IAPV with colony collapse disorder [41], more recent work in the same and other geographic areas as the present study suggests that this virus by itself is not a determinant in colony collapse [42].…”

Section: Discussionmentioning

confidence: 76%

Immune related genes as markers for monitoring health status of honey bee colonies

Barroso-Arévalo

Vicente-Rubiano

Puerta³

et al. 2019

BMC Vet Res

View full text Add to dashboard Cite

Background Honey bee population decline threatens the beekeeping sector, agriculture and global biodiversity. Early detection of colony mortality may facilitate rapid interventions to contain and prevent mortality spread. Among others, deformed wing virus (DWV) is capable of inducing colony losses, especially when combined with Varroa destructor mite. Since the bee immune system plays a crucial role in ensuring that bees are able to face these pathogens, we explored whether expression of immune genes could serve as biomarkers of colony health. Results Herein, we describe a preliminary immunological marker composed of two immune genes ( relish and defensin ), which provide insight on honey bee antiviral defense mechanism. Of the tested genes, relish expression correlated with the presence of DWV-Varroa complex, while decreased defensin expression correlated with poor resistance to this complex. Conclusions The monitoring of these genes may help us to better understand the complex physiology of honey bees’s immune system and to develop new approaches for managing the health impacts of DWV infection and varroa infestation in the field.

show abstract

“…Still, since many viral surveys in colonies around the world detected a substantial prevalence of IAPV in covert infections [16,24,25,28,30,36,38] we decided to study the status of this virus in the CCD colonies that we recovered. …”

Section: Discussionmentioning

confidence: 99%

“…Early-on we detected the presence of significant loads of IAPV in worker bees of these colonies. Given the fact that many viral surveys in colonies around the world pointed to a substantial prevalence of IAPV in covert infections [ 16 , 24 , 25 , 28 , 29 , 36 , 38 ], we decided to study the status of this virus in these CCD colonies. We rescued several of these clinical CCD-colonies, avoiding their immediate collapse, to test the hypothesis if IAPV was actively replicating in them and if the virus was infectious to healthy bees.…”

Section: Introductionmentioning

confidence: 99%

Dynamics of the Presence of Israeli Acute Paralysis Virus in Honey Bee Colonies with Colony Collapse Disorder

Hou

Rivkin

Slabezki

et al. 2014

Viruses

View full text Add to dashboard Cite

The determinants of Colony Collapse Disorder (CCD), a particular case of collapse of honey bee colonies, are still unresolved. Viruses including the Israeli acute paralysis virus (IAPV) were associated with CCD. We found an apiary with colonies showing typical CCD characteristics that bore high loads of IAPV, recovered some colonies from collapse and tested the hypothesis if IAPV was actively replicating in them and infectious to healthy bees. We found that IAPV was the dominant pathogen and it replicated actively in the colonies: viral titers decreased from April to September and increased from September to December. IAPV extracted from infected bees was highly infectious to healthy pupae: they showed several-fold amplification of the viral genome and synthesis of the virion protein VP3. The health of recovered colonies was seriously compromised. Interestingly, a rise of IAPV genomic copies in two colonies coincided with their subsequent collapse. Our results do not imply IAPV as the cause of CCD but indicate that once acquired and induced to replication it acts as an infectious factor that affects the health of the colonies and may determine their survival. This is the first follow up outside the US of CCD-colonies bearing IAPV under natural conditions.

show abstract

Short communication. Presence, quantification and phylogeny of Israeli acute paralysis virus of honeybees in Andalusia (Spain)

Cited by 5 publications

References 17 publications

High Load of Deformed Wing Virus and Varroa destructor Infestation Are Related to Weakness of Honey Bee Colonies in Southern Spain

High Load of Deformed Wing Virus and Varroa destructor Infestation Are Related to Weakness of Honey Bee Colonies in Southern Spain

Immune related genes as markers for monitoring health status of honey bee colonies

Dynamics of the Presence of Israeli Acute Paralysis Virus in Honey Bee Colonies with Colony Collapse Disorder

Contact Info

Product

Resources

About