The activity of T- and B-cell links of specific protection of chicken-broilers under the influence of synbiotic preparation “Biomagn” and “Diolide” disinfectant

Abstract

One of the most actual problems of modern poultry farming is to increase the preservation of chickens and ensure high intensity of their growth at all stages of rearing. The development of alternative methods of prevention and treatment of infectious diseases of poultry involves the use of preparations of natural or organic origin, which have antagonistic properties against infectious agents and are able to balance the immune response. A prominent place in the system of disease prevention and increasing the resistance of animals and poultry is the development of modern probiotic and bactericidal preparations. In view of this, we studied the influence of the synbiotic preparations “Biomagn” in combination with the disinfectant “Diolide” on the state of the T- and B-cell links of the specific immunity of broiler chickens during the period of their rearing. The preparation “Biomagn” is based on representatives of the normal commensal microflora - non-pathogenic lactic acid bacteria with antibacterial and immunomodulatory properties and additional components: magnesium chloride, chitosan, xylanase, protease, cellulase, milk thistle meal, acidity regulator, betaine and emulsifier. The specified remedy is used to ensure optimal level of metabolism, increase immune function, growth, safety of animals and poultry. The results of the research showed that the use of the synbiotic preparation “Biomagn” in combination with the disinfectant “Diolide” had a positive influence on the activity of T- and B-cell links of specific defense of broiler chickens organism during the period of their rearing. This is evidenced by an increase in the amount of T-lymphocytes (total, active and theophylline-resistant) and B-lymphocytes in the blood and an increase in their functional activity due to the redistribution of the receptor apparatus of immunocompetent cells. In particular, a decrease in the number of functionally inactive T- and B-lymphocytes in the blood and an increase in the number of cells with low and medium degree of avidity. Therefore, the use of a set of studied immunotropic preparations is a promising direction in increasing the resistance and productivity of poultry. This allows in industrial conditions of maintenance, without loss of productivity, to respond to technological factors that can lead to immunodeficiency, disease and death of poultry.

The effect of complex application of symbiotic and biocidal preparations on the metabolic status of broiler chickens' blood

“…The content of micro-and macronutrients was explored by inductively coupled plasma optical emission spectrometry using an optical emission spectrometer (ICP-OES) PlasmaQuant PQ 9000 (Germany, 2018). Ultrapure nitric acid (Merck, Germany), certified multi-element standard solutions for atomic emission spectrometry (Merck, Germany) with certified ion content were used to prepare samples and prepare background and calibration solutions (El Kady et al, 2012; Chechet et al, 2022). The solvent was ultra-pure deionised water according to ISO 11885:1996ISO 11885: (1996, prepared by the Atrium 631 UV purification system (Sartorius, Germany).…”

“…Modern disinfectants should ensure the proper state of veterinary and sanitary welfare and reliable protection against infections, thus, they are one of the elements in the prevention of infectious diseases of productive animals and poultry in the technological process of their cultivation. Thus, previous studies by the authors (Chechet et al, 2021;Chechet et al, 2022;Kovalenko et al, 2022) and several other scientists (Melnyk, 2017;Demchyshyn et al, 2018) in in vitro and in vivo experiments have established the effectiveness and safety of biocides based on organic acids, peroxide compounds, chlorine dioxide, etc. As described by J.…”

“…Bailly et al (2017), O.M. Chechet et al (2022) and V.L. Kovalenko et al (2022) have demonstrated that the disinfectants "Diolaid" and "Biolaid" have an active antimicrobial effect against gram-positive and gram-negative bacteria, fungicidal properties, virulent effect and prevent resistance of microorganisms to the drug.…”

Productivity of broiler chickens using the optimal dosage of zinc proteinate

“…На сьогоднішній час успішне ведення птахівництва неможливе без організації повноцінної збалансованої годівлі птиці та застосування різноманітних кормових добавок з широким спектром дії, які різняться між собою за походженням, набором біологічно активних компонентів та технологією виробництва. Додавання їх до раціонів птиці сприяє підвищенню рівня трансформації поживних речовин кормів у продукцію і створює сприятливі умови для максимального генетичного потенціалу їх продуктивності (Ibatullin, 2003;Makaryns'ka & Jegorov, 2010;Djachenko et al, 2015;Sobolev et al, 2021;Chechet et al, 2022).…”

The effect of butaselmevit and closaverm A on the immune status of cows with experimental fasciolosis sensitized by atypical mycobacteria

“…In the literature (Martyshuk & Hutyi, 2021;Zhelavskyi et al, 2022;Chechet et al, 2022) there are many reports about the important role of the immune system in protecting the body against the effects of foreign agents. However, the role of immune status in the association of mycobacteriosis and fasciolosis has not been studied.…”