This study was conducted to investigate the antimicrobial effect of chlorine dioxide (ClO2) on the vase life of cut rose 'Beast' (Rosa hybrida L.). Postharvest treatments to extend the vase life of cut roses were divided into two: holding solution treatment and pulsing solution treatment. In holding solution treatment, the cut roses were treated with preservative solutions containing tap water (TW, control), distilled water (DW), ClO2 2, 4, 6, and 8 µL・L . In pulsing solution treatment, cut roses were dipped into the ClO2 solutions of 50, 100, 150, 200, and 250 µL・L -1 for 60 seconds and were placed in DW. The air temperature was 18.4°C, RH 51.5%, and light (photosynthetically active radiation, PAR) 3.6 µmol・m ). The ClO2 contents in holding solution of all treatments were scavenged in two-four days after treatment. This study indicated that ClO2 4 µL・L -1 holding solution treatment and 200-250 µL・L -1 pulsing solution treatment can be applied to extend the postharvest life of cut roses.